Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,799,764	T→A	I145F (ATC→TTC)	waaZ ←	lipopolysaccharide core biosynthesis protein WaaZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,799,764	0	T	A	100.0%	313.7 / NA	88	I145F (ATC→TTC)	waaZ	lipopolysaccharide core biosynthesis protein WaaZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (53/35);  total (53/35)

CAAATAATCCGCGCATACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGATCAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTATCTTCTTTGGAAGCGT  >  NC_000913/3799628‑3799898                                                                                                                                         |                                                                                                                                       cAAATAATCCGCGCATACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAAcag                                                                                                                                     <  1:602271/140‑1 (MQ=255)  aaaTAATCCGCGCATACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATAAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAAcagc                                                                                                                                    <  1:1209740/140‑1 (MQ=255)      aatCCGCGCATACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGtt                                                                                                                                <  2:584433/140‑1 (MQ=255)        tCCGCGCATACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCt                                                                                                                              >  2:483748/1‑140 (MQ=255)        tCCGCGCATACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCt                                                                                                                              >  1:817347/1‑140 (MQ=255)         ccGCGCATACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCtt                                                                                                                             >  1:175859/1‑140 (MQ=255)         ccGCGCATACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCtt                                                                                                                             >  1:529000/1‑140 (MQ=255)              cATACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGa                                                                                                                        >  1:550488/1‑140 (MQ=255)              cATACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGa                                                                                                                        >  1:1129102/1‑140 (MQ=255)                tACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAAt                                                                                                                      <  2:683117/140‑1 (MQ=255)                tACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAAt                                                                                                                      <  1:423913/140‑1 (MQ=255)                 aCTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACGACGGTACAGAGAACAGCAGTTCTTTGTGAATc                                                                                                                     >  1:1085525/1‑140 (MQ=255)                 aCTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATc                                                                                                                     <  1:707263/140‑1 (MQ=255)                       gTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGtctc                                                                                                               >  1:610026/1‑140 (MQ=255)                        tGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCa                                                                                                              <  2:610026/140‑1 (MQ=255)                           atatGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCaaaa                                                                                                           >  2:436820/1‑140 (MQ=255)                           atatGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCaaaa                                                                                                           >  2:1202033/1‑140 (MQ=255)                           atatGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCaaaa                                                                                                           >  1:1037383/1‑140 (MQ=255)                            tatGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAAt                                                                                                                      <  1:765269/128‑1 (MQ=255)                            tatGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAAt                                                                                                                      >  2:765269/1‑128 (MQ=255)                            tatGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCa                                                                                                              <  2:884682/136‑1 (MQ=255)                            tatGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCa                                                                                                              >  1:884682/1‑136 (MQ=255)                            tatGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAat                                                                                                          >  1:564118/1‑140 (MQ=255)                            tatGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAat                                                                                                          >  1:812091/1‑140 (MQ=255)                             atGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAata                                                                                                         >  2:869641/1‑140 (MQ=255)                             atGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAata                                                                                                         >  2:401702/1‑140 (MQ=255)                              tGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAatat                                                                                                        <  1:401702/140‑1 (MQ=255)                                cGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTa                                                                                                      >  2:699234/1‑140 (MQ=255)                                    ttGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAAtt                                                                                                  >  1:1085273/1‑140 (MQ=255)                                        aTTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAAt                                                                                              >  2:925692/1‑140 (MQ=255)                                        aTTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAAt                                                                                              <  1:699234/140‑1 (MQ=255)                                           gcagcaAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAAtctt                                                                                           <  2:1129102/140‑1 (MQ=255)                                           gcagcaAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAAtctt                                                                                           <  1:789471/140‑1 (MQ=255)                                                           tATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCt                                                                           >  2:981298/1‑140 (MQ=255)                                                             tGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCttt                                                                         >  2:431032/1‑140 (MQ=255)                                                              ggCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCtttt                                                                        >  2:981888/1‑140 (MQ=255)                                                                  tGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTccc                                                                    >  2:715450/1‑140 (MQ=255)                                                                  tGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTccc                                                                    >  1:1180275/1‑140 (MQ=255)                                                                   gAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCg                                                                   >  2:627293/1‑140 (MQ=255)                                                                   gAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCg                                                                   <  1:798613/140‑1 (MQ=255)                                                                   gAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCg                                                                   <  1:720130/140‑1 (MQ=255)                                                                   gAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCg                                                                   <  1:981298/140‑1 (MQ=255)                                                                   gAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCg                                                                   >  1:607241/1‑140 (MQ=255)                                                                   gAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCg                                                                   <  2:1180275/140‑1 (MQ=255)                                                                   gAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGAACTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCg                                                                   >  2:759617/1‑140 (MQ=255)                                                                    aGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGa                                                                  >  2:802181/1‑140 (MQ=255)                                                                    aGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGa                                                                  >  2:229672/1‑140 (MQ=255)                                                                    aGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGa                                                                  >  1:307784/1‑140 (MQ=255)                                                                    aGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGa                                                                  >  1:826808/1‑140 (MQ=255)                                                                    aGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGa                                                                  >  1:671526/1‑140 (MQ=255)                                                                    aGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGa                                                                  >  1:997735/1‑140 (MQ=255)                                                                    aGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGa                                                                  >  2:1007507/1‑140 (MQ=255)                                                                     gCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGAc                                                                 >  2:474257/1‑140 (MQ=255)                                                                     gCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGAc                                                                 <  1:229672/140‑1 (MQ=255)                                                                         tAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCg                                                                   <  2:423798/134‑1 (MQ=255)                                                                         tAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCg                                                                   >  1:1119184/1‑134 (MQ=255)                                                                         tAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCg                                                                   >  1:423798/1‑134 (MQ=255)                                                                         tAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCg                                                                   <  2:1119184/134‑1 (MQ=255)                                                                              aaGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAgg                                                        >  1:211323/1‑140 (MQ=255)                                                                               aGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTCCCCGACGGTAAAAGGa                                                       <  2:288572/140‑1 (MQ=255)                                                                                gACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGAc                                                      >  2:697046/1‑140 (MQ=255)                                                                                gACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGAc                                                      >  1:419221/1‑140 (MQ=255)                                                                                  cTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCg                                                    <  2:585279/140‑1 (MQ=255)                                                                                  cTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCg                                                    <  2:595492/140‑1 (MQ=255)                                                                                  cTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCg                                                    <  2:817347/140‑1 (MQ=255)                                                                                     aTGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTaa                                                 >  2:245195/1‑140 (MQ=255)                                                                                       gTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCACAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATa                                               <  2:1085273/140‑1 (MQ=255)                                                                                          cTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGa                                            >  1:1065418/1‑140 (MQ=255)                                                                                          cTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGa                                            >  1:418354/1‑140 (MQ=255)                                                                                          cTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGa                                            >  2:545477/1‑140 (MQ=255)                                                                                            tGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAgg                                          <  2:120829/140‑1 (MQ=255)                                                                                             gCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGc                                         >  2:707587/1‑140 (MQ=255)                                                                                                 aaTCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGtt                                     <  1:375035/140‑1 (MQ=255)                                                                                                   tCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGGCGGTAAAAGGACCGTAATACGAGGCAGTTCt                                   >  2:645630/1‑140 (MQ=255)                                                                                                   tCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCt                                   >  2:406009/1‑140 (MQ=255)                                                                                                   tCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCt                                   >  2:166891/1‑140 (MQ=255)                                                                                                                 cTTTTTTAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGt                     <  1:697046/140‑1 (MQ=255)                                                                                                                    ttttAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTa                  <  2:997735/140‑1 (MQ=255)                                                                                                                    ttttAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTa                  <  1:436820/140‑1 (MQ=255)                                                                                                                      ttAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTAtc                <  2:1037383/140‑1 (MQ=255)                                                                                                                      ttAGACAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTAtc                <  2:92778/140‑1 (MQ=255)                                                                                                                          acacCGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTAtcttct            >  2:884681/1‑140 (MQ=255)                                                                                                                          aCAACGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTAtcttct            >  1:59728/1‑140 (MQ=255)                                                                                                                              cGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTATCTTCTTTgg        >  2:1078686/1‑140 (MQ=255)                                                                                                                              cGGTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTATCTTCTTTgg        >  1:195479/1‑140 (MQ=255)                                                                                                                                gTACAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTATCTTCTTTGGaa      <  1:869641/140‑1 (MQ=255)                                                                                                                                  aCAGAGAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTATCTTCTTTGGAAGc    <  2:1085525/140‑1 (MQ=255)                                                                                                                                    agagAACAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTATCTTCTTTGGAAGCgt  <  2:564118/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CAAATAATCCGCGCATACTTAAGTGAATATGCGATTTGAATTGCAGCAAAGGCAATAGTATGGCATGAGCAATAACCAAGACTAATGTCCTTGCAAAATCCAACCAGACGACCTTTTTTAGACAACGGTACAGAGATCAGCAGTTCTTTGTGAATCTGTCTCAAAATATTAAATTTAATCTTCTTTATAAAACCACCTTTTTCCCGACGGTAAAAGGACCGTAATACGAGGCAGTTCTGGAGGATATAAAGTTTATCTTCTTTGGAAGCGT  >  NC_000913/3799628‑3799898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑