Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,852,713	T→C	E24E (GAA→GAG)	ilvB ←	acetohydroxy acid synthase I subunit IlvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,852,713	0	T	C	100.0%	182.1 / NA	52	E24E (GAA→GAG)	ilvB	acetohydroxy acid synthase I subunit IlvB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/23);  total (29/23)

CCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGG  >  NC_000913/3852580‑3852847                                                                                                                                      |                                                                                                                                       ccTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAgg                                                                                                                                  <  1:873692/140‑1 (MQ=255)     aGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAAt                                                                                                                              <  2:35921/140‑1 (MQ=255)       cGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGa                                                                                                                            <  1:869211/140‑1 (MQ=255)       cGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGa                                                                                                                            <  2:480506/140‑1 (MQ=255)         aTAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAAc                                                                                                                          <  2:1048602/140‑1 (MQ=255)            aaGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGAt                                                                                                                       >  1:65310/1‑140 (MQ=255)                    gcCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTg                                                                                                               <  1:868780/140‑1 (MQ=255)                    gcCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTg                                                                                                               >  1:714718/1‑140 (MQ=255)                                    gCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCtt                                                                                               >  1:155494/1‑140 (MQ=255)                                    gCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCtt                                                                                               >  1:357498/1‑140 (MQ=255)                                    gCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCCGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCtt                                                                                               >  2:248216/1‑140 (MQ=255)                                           tatGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTc                                                                                        <  2:143102/140‑1 (MQ=255)                                                cGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGAtgt                                                                                   <  2:122137/140‑1 (MQ=255)                                                     ttGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGc                                                                              <  2:646616/140‑1 (MQ=255)                                                      tGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGcc                                                                             <  1:58749/140‑1 (MQ=255)                                                             tttGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACtt                                                                      >  2:935160/1‑140 (MQ=255)                                                              ttGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTg                                                                     >  1:1065955/1‑140 (MQ=255)                                                                      aGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTc                                                             >  2:795040/1‑140 (MQ=255)                                                                         cATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAg                                                          >  1:735397/1‑140 (MQ=255)                                                                           tCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCa                                                           >  2:841641/1‑137 (MQ=255)                                                                           tCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCa                                                           <  1:841641/137‑1 (MQ=255)                                                                           tCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTc                                                        >  2:340237/1‑140 (MQ=255)                                                                           tCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTc                                                        >  1:965928/1‑140 (MQ=255)                                                                           tCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTc                                                        <  1:340237/140‑1 (MQ=255)                                                                                     gcaggATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTc                                                             >  1:1136149/1‑125 (MQ=255)                                                                                     gcaggATAGAACCGCCCGGAATGCCTGCCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTc                                                             <  2:1136149/125‑1 (MQ=255)                                                                                         gATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGc                                          >  2:712291/1‑140 (MQ=255)                                                                                         gATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGc                                          >  2:675240/1‑140 (MQ=255)                                                                                          aTAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGcc                                         <  2:65310/140‑1 (MQ=255)                                                                                            aGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGa                                       <  1:935160/140‑1 (MQ=255)                                                                                             gAACAGCCCGGAATGCCTGTCACAATCTTAATGCCTTGCTGTTCCAGGAAATGAACGATAAAATCTGCGTCGGTAAAGCGCTGTCGCGTCGATGTTGTGCCCGAACTGGCCATGCTCCATTCCTTTTTTGCTGGGCCTAc                                      <  2:490788/140‑1 (MQ=255)                                                                                              aaCCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACt                                     >  1:375441/1‑140 (MQ=255)                                                                                                 cGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTc                                  <  1:712291/140‑1 (MQ=255)                                                                                                   cccGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCg                                >  2:979042/1‑140 (MQ=255)                                                                                                   cccGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCg                                >  1:423650/1‑140 (MQ=255)                                                                                                   cccGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCg                                >  2:31970/1‑140 (MQ=255)                                                                                                   cccGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCg                                >  1:466864/1‑140 (MQ=255)                                                                                                   cccGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCg                                >  1:652770/1‑140 (MQ=255)                                                                                                   cccGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCg                                >  1:742649/1‑140 (MQ=255)                                                                                                   cccGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCg                                >  1:747532/1‑140 (MQ=255)                                                                                                   cccGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCAAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCg                                >  1:369413/1‑140 (MQ=255)                                                                                                     cGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCggg                              <  1:1181670/140‑1 (MQ=255)                                                                                                     cGGAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCggg                              <  2:965928/140‑1 (MQ=255)                                                                                                      ggAATGCCTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGa                             <  2:851293/140‑1 (MQ=255)                                                                                                            ccTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCt                       >  1:341674/1‑140 (MQ=255)                                                                                                             cTGTCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCtt                      <  2:466864/140‑1 (MQ=255)                                                                                                                tCACAATCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTaaa                   >  1:466041/1‑140 (MQ=255)                                                                                                                    aaTCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGaa               <  2:652770/140‑1 (MQ=255)                                                                                                                     aTCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGaaa              <  1:80579/140‑1 (MQ=255)                                                                                                                     aTCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGaaa              >  1:69967/1‑140 (MQ=255)                                                                                                                      tCTTAATGCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGaaaa             >  1:844547/1‑140 (MQ=255)                                                                                                                             gCCCTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACcccgc      <  2:375441/140‑1 (MQ=255)                                                                                                                              cccTGCTGCTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACcccgcc     >  1:418944/1‑140 (MQ=255)                                                                                                                                 tgctgcTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCgg  >  2:39217/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       CCTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAGGAAATGAACGATAAATTCTGCGCCGGTAAAGCGCTTACGCGTCGATGTTGTGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGG  >  NC_000913/3852580‑3852847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑