Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 424,176 A→G V553V (GTA→GTG)  malZ → maltodextrin glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913424,1760AG98.1% 177.0 / ‑4.3 52V553V (GTA→GTG) malZmaltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (20/31);  total (21/31)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

GCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCG  >  NC_000913/424042‑424311
                                                                                                                                      |                                                                                                                                       
gCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCtggt                                                                                                                                    <  2:597705/140‑1 (MQ=255)
gCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCtggt                                                                                                                                    <  1:895824/140‑1 (MQ=255)
      ttCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAAt                                                                                                                              <  2:953879/140‑1 (MQ=255)
      ttCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAAt                                                                                                                              <  1:314741/140‑1 (MQ=255)
                     cGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGc                                                                                                               <  2:240841/140‑1 (MQ=255)
                         tGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCtgt                                                                                                           >  2:354903/1‑140 (MQ=255)
                                gcTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgtgg                                                                                                    <  2:1108879/140‑1 (MQ=255)
                                      tAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTAc                                                                                              <  2:1009015/140‑1 (MQ=255)
                                            aaGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGt                                                                                        <  2:1075185/140‑1 (MQ=255)
                                              gTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCa                                                                                      >  1:1143226/1‑140 (MQ=255)
                                              gTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCa                                                                                      >  2:923081/1‑140 (MQ=255)
                                              gTCAGGCGCTACGTCATGGCGGCTGTCAGGAGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCa                                                                                      <  2:744138/140‑1 (MQ=255)
                                                cAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCAcc                                                                                    <  1:631754/140‑1 (MQ=255)
                                                 aGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCg                                                                                   <  1:198944/140‑1 (MQ=255)
                                                  ggCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGt                                                                                  <  1:875467/140‑1 (MQ=255)
                                                   gcgcTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGt                                                                                        <  2:751722/133‑1 (MQ=255)
                                                   gcgcTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGt                                                                                        >  1:751722/1‑133 (MQ=255)
                                                   gcgcTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGtt                                                                                 >  1:544482/1‑140 (MQ=255)
                                                   gcgcTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGtt                                                                                 >  2:334362/1‑140 (MQ=255)
                                                   gcgcTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAACAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGt                                                                                        >  1:478849/1‑133 (MQ=255)
                                                   gcgcTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAACAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGt                                                                                        <  2:478849/133‑1 (MQ=255)
                                                        aCGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCa                                                                            <  1:698764/140‑1 (MQ=255)
                                                           tCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATg                                                                         <  1:714392/140‑1 (MQ=255)
                                                            cATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGc                                                                        <  1:234078/140‑1 (MQ=255)
                                                            cATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGc                                                                        <  2:431083/140‑1 (MQ=255)
                                                             aTGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGcc                                                                       >  1:46174/1‑140 (MQ=255)
                                                                      gTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatgg                                                              <  2:544482/140‑1 (MQ=255)
                                                                      gTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatgg                                                              <  2:1143226/140‑1 (MQ=255)
                                                                        cAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggca                                                            <  1:84170/140‑1 (MQ=255)
                                                                        cAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggca                                                            <  2:230912/140‑1 (MQ=255)
                                                                              cTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCaa                                                      >  1:37063/1‑140 (MQ=255)
                                                                                 tATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaaga                                                   <  1:923081/140‑1 (MQ=255)
                                                                                 tATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaag                                                    >  2:453540/1‑139 (MQ=255)
                                                                                 tATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaag                                                    <  1:453540/139‑1 (MQ=255)
                                                                                     cGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAggg                                               >  1:171002/1‑140 (MQ=255)
                                                                                      ggAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGc                                              <  2:117403/140‑1 (MQ=255)
                                                                                         aGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATg                                           >  2:219638/1‑140 (MQ=255)
                                                                                           aTAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATggg                                         >  1:387423/1‑140 (MQ=255)
                                                                                           aTAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATggg                                         >  2:1209340/1‑140 (MQ=255)
                                                                                              aCGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCAt                                            >  2:712751/1‑134 (MQ=255)
                                                                                              aCGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCAt                                            <  1:712751/134‑1 (MQ=255)
                                                                                              aCGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCaa                                      <  1:627412/140‑1 (MQ=255)
                                                                                                   gtggtATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCGAAGAAGGGCATGGGCAactgac                                 <  1:686938/140‑1 (MQ=255)
                                                                                                   gtggtATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactgac                                 <  1:1056546/140‑1 (MQ=255)
                                                                                                    tggtATTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactgact                                >  1:299477/1‑140 (MQ=255)
                                                                                                        aTTTGTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACg                            <  1:334362/140‑1 (MQ=255)
                                                                                                            gTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGAt                        >  1:659257/1‑140 (MQ=255)
                                                                                                            gTCCGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGAt                        >  2:1101570/1‑140 (MQ=255)
                                                                                                              ccGCGTGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTc                      >  2:1177550/1‑140 (MQ=255)
                                                                                                                   tGCTGAATCAGCAACGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCt                 >  2:206921/1‑140 (MQ=255)
                                                                                                                               aaCGTGTGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCAtt     <  2:387423/140‑1 (MQ=255)
                                                                                                                                  gtgtGCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCg  >  2:125784/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GCGTTATTCGCGCTGTACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCG  >  NC_000913/424042‑424311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑