Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,918,383 T→C Y492C (TAC→TGC)  atpA ← ATP synthase F1 complex subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,918,3830TC100.0% 232.3 / NA 65Y492C (TAC→TGC) atpAATP synthase F1 complex subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/33);  total (32/33)

CTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAA  >  NC_000913/3918248‑3918518
                                                                                                                                       |                                                                                                                                       
ctctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGcc                                                                                                                                     >  2:582728/1‑140 (MQ=255)
  ctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGcccc                                                                                                                                   <  2:481770/140‑3 (MQ=255)
  ctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCAc                                                                                                                                   <  1:1113933/140‑1 (MQ=255)
  ctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGC‑TTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCAcc                                                                                                                                  >  2:903014/1‑140 (MQ=255)
     ttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCgg                                                                                                                                >  1:197332/1‑140 (MQ=255)
      tGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCa                                                                                                                                    >  2:1146011/1‑135 (MQ=255)
      tGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCa                                                                                                                                    <  1:1146011/135‑1 (MQ=255)
      tGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGt                                                                                                                               >  2:57838/1‑140 (MQ=255)
           cGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGC‑TTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGtt                                                                                                                         <  1:903014/140‑1 (MQ=255)
           cGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGCTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGt                                                                                                                          <  1:39773/140‑1 (MQ=255)
            ggCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGtt                                                                                                                         <  1:1138646/140‑1 (MQ=255)
             gCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTg                                                                                                                        >  1:1049840/1‑140 (MQ=255)
                   aGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCGCCGGTCTGGTTGATctct                                                                                                                  <  2:286560/140‑1 (MQ=255)
                        ctcctcAATGCCTTGCGGCCTGCCCTAAGGCAAGTCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCAt                                                                                                             >  2:1076569/1‑140 (MQ=255)
                        ctcctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCAt                                                                                                             >  2:517334/1‑140 (MQ=255)
                           ctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCaa                                                                                                          <  1:605535/140‑1 (MQ=255)
                           ctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCaa                                                                                                          <  1:582728/140‑1 (MQ=255)
                            tcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTGTCAGCTTGCCTTCGATTTCGTCGGGGCAGCCGCCGGGCTGGGTGAGCTCTTGCAGCAAc                                                                                                         <  2:1166271/140‑1 (MQ=255)
                              aaTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACgg                                                                                                       <  1:659491/140‑1 (MQ=255)
                                tGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAg                                                                                                     >  2:118608/1‑140 (MQ=255)
                                 gCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGc                                                                                                    <  1:247114/140‑1 (MQ=255)
                                 gCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGc                                                                                                    <  2:720902/140‑1 (MQ=255)
                                   cTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGt                                                                                                  >  1:1146114/1‑140 (MQ=255)
                                    ttGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTg                                                                                                 <  1:517334/140‑1 (MQ=255)
                                     tGCGGCCTGCCCTAAGGCAAGCTGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGa                                                                                                >  1:563512/1‑140 (MQ=255)
                                            tGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGt                                                                                         <  2:763155/140‑1 (MQ=255)
                                            tGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGt                                                                                         <  1:898850/140‑1 (MQ=255)
                                             gCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGTATCAACGGAGCGTGATCACGGTc                                                                                        <  1:57838/140‑1 (MQ=255)
                                             gCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTc                                                                                        >  1:690823/1‑140 (MQ=255)
                                                 tAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACg                                                                                    <  1:45057/140‑1 (MQ=255)
                                                 tAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACg                                                                                    <  2:1140807/140‑1 (MQ=255)
                                                      cAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGc                                                                               <  2:1340/140‑1 (MQ=255)
                                                        aGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCca                                                                             >  2:955141/1‑140 (MQ=255)
                                                        aGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCca                                                                             >  2:745920/1‑140 (MQ=255)
                                                        aGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCca                                                                             >  1:784289/1‑140 (MQ=255)
                                                        aGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCca                                                                             >  1:887109/1‑140 (MQ=255)
                                                        aGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCca                                                                             >  1:39592/1‑140 (MQ=255)
                                                        aGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCca                                                                             >  1:507201/1‑140 (MQ=255)
                                                        aGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCca                                                                             >  1:604187/1‑140 (MQ=255)
                                                         gccgccAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGc                                                                               <  1:163005/137‑1 (MQ=255)
                                                         gccgccAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGc                                                                               >  2:163005/1‑137 (MQ=255)
                                                                      aCCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCg                                                               >  2:490516/1‑140 (MQ=255)
                                                                      aCCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCg                                                               >  2:103926/1‑140 (MQ=255)
                                                                             ttGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGc                                                        <  1:514370/140‑1 (MQ=255)
                                                                             ttGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGc                                                        <  1:118608/140‑1 (MQ=255)
                                                                             ttGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGc                                                        <  2:800630/140‑1 (MQ=255)
                                                                               gggTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCa                                                      >  1:399850/1‑140 (MQ=255)
                                                                                ggTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGCCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCaa                                                     >  2:178445/1‑140 (MQ=255)
                                                                                 gTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAAt                                                    >  2:1036115/1‑140 (MQ=255)
                                                                                     cTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTg                                                         <  2:295514/131‑1 (MQ=255)
                                                                                     cTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTg                                                         >  1:295514/1‑131 (MQ=255)
                                                                                     cTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTc                                                >  1:1206341/1‑140 (MQ=255)
                                                                                       ttGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGa                                              <  1:490516/140‑1 (MQ=255)
                                                                                        tGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGAc                                             <  1:1036115/140‑1 (MQ=255)
                                                                                                   aGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATc                                  >  1:1056665/1‑140 (MQ=255)
                                                                                                      aTGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGc                               >  2:821439/1‑140 (MQ=255)
                                                                                                      aTGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGc                               <  1:1077904/140‑1 (MQ=255)
                                                                                                                   tGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGt                  >  1:380849/1‑140 (MQ=255)
                                                                                                                   tGCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGt                  >  2:986514/1‑140 (MQ=255)
                                                                                                                    gCCTTCGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGtt                 <  2:784289/140‑1 (MQ=255)
                                                                                                                         cGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTctgct            >  1:207911/1‑140 (MQ=255)
                                                                                                                         cGATTTCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTctgct            <  1:742894/140‑1 (MQ=255)
                                                                                                                            tttCGTCGTTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCg         <  1:745920/140‑1 (MQ=255)
                                                                                                                                 tcgtTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAg    <  2:207911/140‑1 (MQ=255)
                                                                                                                                   gtTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGaa  <  2:39592/140‑1 (MQ=255)
                                                                                                                                   gtTGCAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGaa  <  2:197332/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAA  >  NC_000913/3918248‑3918518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑