Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,924,789	T→C	D282G (GAC→GGC)	mnmG ←	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,924,789	0	T	C	100.0%	274.2 / NA	77	D282G (GAC→GGC)	mnmG	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/42);  total (35/42)

AGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGG  >  NC_000913/3924655‑3924923                                                                                                                                       |                                                                                                                                       agCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTtcg                                                                                                                                   >  1:827363/1‑140 (MQ=255) agCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTtcg                                                                                                                                   >  2:744693/1‑140 (MQ=255) agCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTtcg                                                                                                                                   >  1:343892/1‑140 (MQ=255) agCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTtcg                                                                                                                                   >  2:165122/1‑140 (MQ=255) agCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTtcg                                                                                                                                   >  2:171684/1‑140 (MQ=255) agCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTtcg                                                                                                                                   >  2:219097/1‑140 (MQ=255)      aCGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTtcgatcga                                                                                                                              >  1:1082666/1‑140 (MQ=255)      aCGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTtcgatcga                                                                                                                              >  1:853950/1‑140 (MQ=255)       cGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGAc                                                                                                                             <  1:804116/140‑1 (MQ=255)       cGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGAc                                                                                                                             <  1:744693/140‑1 (MQ=255)          tttGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGAc                                                                                                                             >  2:457194/1‑137 (MQ=255)          tttGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGAc                                                                                                                             <  1:457194/137‑1 (MQ=255)          tttGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACggg                                                                                                                          >  1:1108593/1‑140 (MQ=255)           ttGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGc                                                                                                                         >  2:216222/1‑140 (MQ=255)            tGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCa                                                                                                                        <  2:343892/140‑1 (MQ=255)              cATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGt                                                                                                                      <  1:714570/140‑1 (MQ=255)                    cacaTCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTg                                                                                                                >  1:628059/1‑140 (MQ=255)                          gAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGa                                                                                                          <  2:3455/140‑1 (MQ=255)                          gAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGa                                                                                                          <  2:1058961/140‑1 (MQ=255)                          gAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGa                                                                                                          >  1:583132/1‑140 (MQ=255)                           aaCGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGac                                                                                                         >  2:989667/1‑140 (MQ=255)                             cGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGacac                                                                                                       <  2:1082666/140‑1 (MQ=255)                                 aGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTc                                                                                                   <  1:538487/140‑1 (MQ=255)                                   gCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGa                                                                                                 >  1:421682/1‑140 (MQ=255)                                    cTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGAt                                                                                                <  1:374046/140‑1 (MQ=255)                                      ggtggAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCa                                                                                              <  1:216222/140‑1 (MQ=255)                                      ggtggAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCa                                                                                              <  2:459227/140‑1 (MQ=255)                                      ggtggAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCa                                                                                              <  1:36845/140‑1 (MQ=255)                                      ggtggAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCa                                                                                              <  1:108650/140‑1 (MQ=255)                                        tggAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCAcc                                                                                            <  2:583132/140‑1 (MQ=255)                                         ggAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCAccc                                                                                           <  2:478924/140‑1 (MQ=255)                                           agaTACCGTTCGGCTGAGTTGCATTAGAGGTGAGTCCTTCCGGTTCAAGGGAGATCTGATGCTGATTACTGTCGGCGGCTCGTATGTCTTTGCCTTGGATCGACGGGCAGTGGCGTGGGCCAACACCTTCGATCACCCCt                                                                                         <  2:901708/140‑1 (MQ=255)                                            gaTACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTg                                                                                        <  2:770506/140‑1 (MQ=255)                                             aTACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGAC‑GGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCg                                                                                      >  1:1176315/1‑140 (MQ=255)                                                  gTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGGCTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACa                                                                                  <  1:167182/140‑1 (MQ=255)                                                  gTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACa                                                                                  >  2:847694/1‑140 (MQ=255)                                                  gTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACa                                                                                  >  2:895311/1‑140 (MQ=255)                                                   ttCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACAt                                                                                 <  1:596036/140‑1 (MQ=255)                                                     cGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTg                                                                               >  2:1103451/1‑140 (MQ=255)                                                        aTAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGc                                                                            <  2:628059/140‑1 (MQ=255)                                                              ttCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGAt                                                                      <  1:512490/140‑1 (MQ=255)                                                                 aTTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGa                                                                   <  1:778311/140‑1 (MQ=255)                                                                 aTTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGa                                                                   <  1:16483/140‑1 (MQ=255)                                                                      agGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAgg                                                                 >  1:480087/1‑137 (MQ=255)                                                                      agGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAgg                                                                 <  2:480087/137‑1 (MQ=255)                                                                      agGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTa                                                              <  1:219097/140‑1 (MQ=255)                                                                      agGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTa                                                              <  1:847694/140‑1 (MQ=255)                                                                      agGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTa                                                              <  1:304250/140‑1 (MQ=255)                                                                        gTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACt                                                            >  1:896484/1‑140 (MQ=255)                                                                         tCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGAC‑GGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGc                                                          <  2:1176315/140‑1 (MQ=255)                                                                          cAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGc                                                          <  2:896484/140‑1 (MQ=255)                                                                           aGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCg                                                         >  2:161792/1‑140 (MQ=255)                                                                             tCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTg                                                           >  2:577861/1‑136 (MQ=255)                                                                             tCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTg                                                           <  1:577861/136‑1 (MQ=255)                                                                                  ccGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATcaca                                                  >  1:86170/1‑140 (MQ=255)                                                                                       tCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATg                                             >  2:693770/1‑140 (MQ=255)                                                                                       tCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATg                                             >  2:636175/1‑140 (MQ=255)                                                                                       tCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATg                                             >  1:384030/1‑140 (MQ=255)                                                                                             aaGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGtttt                                       <  1:738805/140‑1 (MQ=255)                                                                                                aTCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGAt                                                      >  1:467205/1‑122 (MQ=255)                                                                                                aTCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGAt                                                      <  2:467205/122‑1 (MQ=255)                                                                                                aTCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATc                                                     >  1:272907/1‑123 (MQ=255)                                                                                                aTCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATc                                                     <  2:272907/123‑1 (MQ=255)                                                                                                  cTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGt                                  <  2:853950/140‑1 (MQ=255)                                                                                                   tGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGtt                                 >  2:152190/1‑140 (MQ=255)                                                                                                     aTGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTgg                               >  2:257639/1‑140 (MQ=255)                                                                                                      tGCTGATTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGt                              <  1:257639/140‑1 (MQ=255)                                                                                                           aTTTCTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAg                         <  2:421682/140‑1 (MQ=255)                                                                                                               cTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGa                          >  2:723445/1‑135 (MQ=255)                                                                                                               cTGTCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGa                          <  1:723445/135‑1 (MQ=255)                                                                                                                  tCGGCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGAtata                  >  1:522709/1‑140 (MQ=255)                                                                                                                     gCGAAGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAaca               <  2:86170/140‑1 (MQ=255)                                                                                                                         aGCGCATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGc           <  1:152190/140‑1 (MQ=255)                                                                                                                            gcATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCAcc        >  1:406205/1‑140 (MQ=255)                                                                                                                            gcATGACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCAcc        >  1:10529/1‑140 (MQ=255)                                                                                                                                gACTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACctgct    <  1:762733/140‑1 (MQ=255)                                                                                                                                  cTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTgg  <  2:849103/140‑1 (MQ=255)                                                                                                                                  cTTTGCCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTgg  <  1:895311/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AGCGGACGATTTGCATCTGCACATCGAACGGCAGGCTGGTGGAGATACCGTTCGGATAAATTTCATTAGAGGTCAGTCCTTCCGGTTCAAGGAAGATCTGATGCTGATTTCTGTCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGG  >  NC_000913/3924655‑3924923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑