Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	424,314	T→C	A599A (GCT→GCC)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	424,314	0	T	C	98.4%	210.8 / ‑4.4	62	A599A (GCT→GCC)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (35/25);  total (36/26)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

TGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTCACGCAGGGCATC  >  NC_000913/424178‑424450                                                                                                                                         |                                                                                                                                         tggtggCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACg                                                                                                                                       >  2:631626/1‑140 (MQ=255)     ggCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTAt                                                                                                                                   >  1:779074/1‑140 (MQ=255)      gCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATg                                                                                                                                  >  1:37825/1‑140 (MQ=255)          tCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATg                                                                                                                              >  2:1212057/1‑140 (MQ=255)             aCCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAAc                                                                                                                           <  1:206921/140‑1 (MQ=255)             aCCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAAc                                                                                                                           <  1:1212057/140‑1 (MQ=255)                gTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTaa                                                                                                                        >  2:453988/1‑140 (MQ=255)                 tGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAAc                                                                                                                       <  2:779074/140‑1 (MQ=255)                      aGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGtttt                                                                                                                  >  1:1053467/1‑140 (MQ=255)                        gCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTtat                                                                                                                <  2:870226/140‑1 (MQ=255)                             tgAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATTGGCAACTGACTGACGGGATTCTGGCTTTGCCTACCATTTCGGCCACGGTATGGATGAACTAACGtttt                                                                                                                  <  1:97770/133‑1 (MQ=255)                             tgAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATTGGCAACTGACTGACGGGATTCTGGCTTTGCCTACCATTTCGGCCACGGTATGGATGAACTAACGtttt                                                                                                                  >  2:97770/1‑133 (MQ=255)                                    gtgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGt                                                                                                    >  1:858325/1‑140 (MQ=255)                                    gtgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATAGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATCGATGAACTAACGTTTTATAACGCCTTGCGt                                                                                                    >  2:743660/1‑140 (MQ=255)                                         aCCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAAc                                                                                                             >  1:362689/1‑126 (MQ=255)                                         aCCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAAc                                                                                                             <  2:362689/126‑1 (MQ=255)                                           ccGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCt                                                                                             <  1:354903/140‑1 (MQ=255)                                            cgcgTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTg                                                                                            >  1:1065868/1‑140 (MQ=255)                                              cgTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTgcg                                                                                          >  2:91034/1‑140 (MQ=255)                                              cgTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTgcg                                                                                          >  2:312823/1‑140 (MQ=255)                                                     gTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCcatcat                                                                                   <  2:1053467/140‑1 (MQ=255)                                                       ttCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACTGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATcc                                                                                 <  2:46174/140‑1 (MQ=255)                                                       ttCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATcc                                                                                 <  2:37825/140‑1 (MQ=255)                                                              tGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATa                                                                          >  1:1032221/1‑140 (MQ=255)                                                                 cGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATaaaa                                                                       <  2:659257/140‑1 (MQ=255)                                                                 cGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATaaaa                                                                       >  2:783228/1‑140 (MQ=255)                                                                   tGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCcatca                                                                                    <  1:834872/125‑1 (MQ=255)                                                                   tGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACt                                                                     >  1:847329/1‑140 (MQ=255)                                                                   tGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACt                                                                     >  2:628437/1‑140 (MQ=255)                                                                   tGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACt                                                                     >  1:1059838/1‑140 (MQ=255)                                                                   tGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGAGTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATGACGCCTTGCGTGACGCCTGCGCcatca                                                                                    >  2:834872/1‑125 (MQ=255)                                                                       atggcaatgCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCa                                                                 <  1:628437/140‑1 (MQ=255)                                                                                cAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGt                                                        <  1:959830/140‑1 (MQ=255)                                                                                   agaagGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTc                                                     >  1:4101/1‑140 (MQ=255)                                                                                       gggCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAgg                                                 <  1:219638/140‑1 (MQ=255)                                                                                       gggCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAgg                                                 <  1:91034/140‑1 (MQ=255)                                                                                       gggCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAgg                                                 <  2:1065868/140‑1 (MQ=255)                                                                                        ggCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAggg                                                >  1:948149/1‑140 (MQ=255)                                                                                        ggCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAggg                                                >  1:789284/1‑140 (MQ=255)                                                                                         gCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGc                                               >  1:751943/1‑140 (MQ=255)                                                                                          cATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCg                                              >  2:1011221/1‑140 (MQ=255)                                                                                          cATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCg                                              >  1:11350/1‑140 (MQ=255)                                                                                               gCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCggg                                                         >  2:1201899/1‑124 (MQ=255)                                                                                               gCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCggg                                                         <  1:1201899/124‑1 (MQ=255)                                                                                                  actgactgACGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATg                                      <  1:783228/140‑1 (MQ=255)                                                                                                   ctgactgaCGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGa                                     >  2:661733/1‑140 (MQ=255)                                                                                                    tgactgacGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAg                                    <  2:1032221/140‑1 (MQ=255)                                                                                                        tgacGGGATTCTGGCTTTGCCTGCCATTTCGGC‑‑CGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTa                              >  1:948407/1‑140 (MQ=255)                                                                                                           cGGGATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTaa                             >  1:1047163/1‑140 (MQ=255)                                                                                                             ggATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAAt                           <  2:299477/140‑1 (MQ=255)                                                                                                              gATTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATc                          >  1:281944/1‑140 (MQ=255)                                                                                                               aTTCTGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCg                         >  2:427362/1‑140 (MQ=255)                                                                                                                   tGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTAcc                     <  2:171002/140‑1 (MQ=255)                                                                                                                   tGGCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTAcc                     <  2:1047163/140‑1 (MQ=255)                                                                                                                     gCTTTGCCTGCCATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAg                   <  1:631626/140‑1 (MQ=255)                                                                                                                         tgcctgccATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGt               >  1:995789/1‑140 (MQ=255)                                                                                                                             tgccATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCAGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTCACg           <  1:743660/140‑1 (MQ=255)                                                                                                                               ccATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTCACGCa         >  1:727881/1‑140 (MQ=255)                                                                                                                                cATTTCGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTCACGCAg        >  1:160968/1‑140 (MQ=255)                                                                                                                                     cGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTCACGCAGGGCAt   >  1:584600/1‑140 (MQ=255)                                                                                                                                     cGGCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTCACGCAGGGCAt   >  1:527328/1‑140 (MQ=255)                                                                                                                                      ggCCACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTCACGCAGGGCATc  <  1:1209340/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTCACGCAGGGCATC  >  NC_000913/424178‑424450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑