Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,155,920	A→G	D307G (GAC→GGC)	argC →	N‑acetylglutamylphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,155,920	0	A	G	100.0%	285.9 / NA	80	D307G (GAC→GGC)	argC	N‑acetylglutamylphosphate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/47);  total (33/47)

AGGCGTATGCCCATAAACCGCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGA  >  NC_000913/4155785‑4156056                                                                                                                                        |                                                                                                                                         agGCGTATGCCCATAAACCGCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAAc                                                                                                                                      <  2:967052/140‑1 (MQ=255)  ggCGTATGCCCATAAACCGCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACt                                                                                                                                     >  1:524432/1‑140 (MQ=255)    cGTATGCCCATAAACCGCTGGTGCGGCTGTATGACAAAGGCGTTCCGACGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTa                                                                                                                                   <  2:985410/140‑1 (MQ=255)          cccATAAACCGCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGaaa                                                                                                                             >  1:648866/1‑140 (MQ=255)            cATAAACCGCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAgg                                                                                                                           <  1:790507/140‑1 (MQ=255)              tAAACCGCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGgcg                                                                                                                         >  2:867785/1‑140 (MQ=255)                     cTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCggcggc                                                                                                                  <  1:1085036/140‑1 (MQ=255)                     cTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCggcggc                                                                                                                  >  2:582660/1‑140 (MQ=255)                     cTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCggcggc                                                                                                                  >  2:540510/1‑140 (MQ=255)                     cTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCggcggc                                                                                                                  <  2:524432/140‑1 (MQ=255)                     cTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCggcggc                                                                                                                  <  2:1098846/140‑1 (MQ=255)                     cTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCggcggc                                                                                                                  >  1:86724/1‑140 (MQ=255)                     cTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCggcggc                                                                                                                  <  1:468864/140‑1 (MQ=255)                       ggTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGcac                                                                                                                <  1:867785/140‑1 (MQ=255)                       ggTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGcac                                                                                                                <  1:743599/140‑1 (MQ=255)                         tGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACaa                                                                                                              <  2:285139/140‑1 (MQ=255)                          gCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAg                                                                                                             <  1:861898/140‑1 (MQ=255)                               tGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTa                                                                                                        <  2:1024851/140‑1 (MQ=255)                                gTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTAc                                                                                                       >  1:838119/1‑140 (MQ=255)                                 tATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACa                                                                                                      <  1:737296/140‑1 (MQ=255)                                  aTGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAg                                                                                                     >  1:149874/1‑140 (MQ=255)                                   tGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGcac                                                                                                                >  2:779702/1‑128 (MQ=255)                                   tGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGcac                                                                                                                <  1:779702/128‑1 (MQ=255)                                   tGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGcac                                                                                                                >  2:301056/1‑128 (MQ=255)                                   tGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGcac                                                                                                                <  1:301056/128‑1 (MQ=255)                                   tGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGt                                                                                                    <  1:1145891/140‑1 (MQ=255)                                        aaGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGTCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGcc                                                                                               >  2:1058303/1‑140 (MQ=255)                                        aaGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGcc                                                                                               >  1:622644/1‑140 (MQ=255)                                         aGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCa                                                                                              >  1:638860/1‑140 (MQ=255)                                           gCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAat                                                                                            >  2:447996/1‑140 (MQ=255)                                           gCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAat                                                                                            >  1:303399/1‑140 (MQ=255)                                           gCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAat                                                                                            >  1:160257/1‑140 (MQ=255)                                           gCGTTCCGGCGCTGAAAAATGTAGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCa                                                                                              <  2:534837/138‑1 (MQ=255)                                           gCGTTCCGGCGCTGAAAAATGTAGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCa                                                                                              >  1:534837/1‑138 (MQ=255)                                            cGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAata                                                                                           <  1:19600/140‑1 (MQ=255)                                                   gcgcTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGttt                                                                                    <  2:303399/140‑1 (MQ=255)                                                        gAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCt                                                                               <  2:160257/140‑1 (MQ=255)                                                        gAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCt                                                                               <  2:622644/140‑1 (MQ=255)                                                                tCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGaa                                                                       <  2:149874/140‑1 (MQ=255)                                                                   ttGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAAcc                                                                    >  2:350736/1‑139 (MQ=255)                                                                   ttGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACg                                                                    >  1:445122/1‑140 (MQ=255)                                                                     gggCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCa                                                                  <  1:615630/140‑1 (MQ=255)                                                                     gggCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCa                                                                  <  2:638860/140‑1 (MQ=255)                                                                     gggCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCa                                                                  <  1:447996/140‑1 (MQ=255)                                                                     gggCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCa                                                                  <  1:785966/140‑1 (MQ=255)                                                                          gCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGtctc                                                             <  2:187350/140‑1 (MQ=255)                                                                          gCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGtctc                                                             <  2:648866/140‑1 (MQ=255)                                                                          gCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGtctc                                                             <  2:769476/140‑1 (MQ=255)                                                                          gCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGtctc                                                             <  1:1201284/140‑1 (MQ=255)                                                                                  gCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTaa                                                     <  2:86724/140‑1 (MQ=255)                                                                                  gCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTaa                                                     <  1:540510/140‑1 (MQ=255)                                                                                  gCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTaa                                                     <  2:677848/140‑1 (MQ=255)                                                                                     atatCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGTGGAAACGCAGTCTCTTATTTAAggg                                                  >  2:156369/1‑140 (MQ=255)                                                                                       atCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTg                                                <  2:1132734/140‑1 (MQ=255)                                                                                        tCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGc                                               >  1:329563/1‑140 (MQ=255)                                                                                         cGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCa                                              >  2:781457/1‑140 (MQ=255)                                                                                           ggTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAat                                            >  1:181369/1‑140 (MQ=255)                                                                                              ttGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAatgat                                         <  1:88231/140‑1 (MQ=255)                                                                                               tGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAatgatg                                        <  2:971159/140‑1 (MQ=255)                                                                                                  cGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAAt                                     >  2:255719/1‑140 (MQ=255)                                                                                                           cGAGCATCTGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCCATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCattaatt                            >  1:440942/1‑140 (MQ=255)                                                                                                                   tGATTATTGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACt                    >  1:1044998/1‑140 (MQ=255)                                                                                                                      ttattGTGGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTggg                 <  2:445122/140‑1 (MQ=255)                                                                                                                           gtgGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGgcggcg            <  2:1103584/140‑1 (MQ=255)                                                                                                                           gtgGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGgcggcg            <  2:181369/140‑1 (MQ=255)                                                                                                                           gtgGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGgcggcg            <  1:964034/140‑1 (MQ=255)                                                                                                                            tgGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGgcggcg            <  2:624341/139‑1 (MQ=255)                                                                                                                            tgGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGgcggcg            >  1:624341/1‑139 (MQ=255)                                                                                                                            tgGCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGt           >  2:253637/1‑140 (MQ=255)                                                                                                                             ggCGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTa          <  2:931729/140‑1 (MQ=255)                                                                                                                               cGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGTGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTAct        <  1:156369/140‑1 (MQ=255)                                                                                                                               cGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGgcggcg            <  1:528218/136‑1 (MQ=255)                                                                                                                               cGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGgcggcg            >  2:528218/1‑136 (MQ=255)                                                                                                                               cGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGgcggcg            <  1:112432/136‑1 (MQ=255)                                                                                                                               cGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGgcggcg            >  2:112432/1‑136 (MQ=255)                                                                                                                               cGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTAct        >  1:6464/1‑140 (MQ=255)                                                                                                                               cGACCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTAct        >  1:683204/1‑140 (MQ=255)                                                                                                                                 aCCGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTActgc      <  2:838119/140‑1 (MQ=255)                                                                                                                                   cGAAGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTActgctg    >  2:472260/1‑140 (MQ=255)                                                                                                                                     aaGGCAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGa  <  2:1205624/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         AGGCGTATGCCCATAAACCGCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGA  >  NC_000913/4155785‑4156056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑