Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,244,166 A→G C304R (TGT→CGT)  malF ← maltose ABC transporter membrane subunit MalF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,244,1660AG98.4% 210.6 / ‑6.1 61C304R (TGT→CGT) malFmaltose ABC transporter membrane subunit MalF
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (31/29);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGG  >  NC_000913/4244030‑4244300
                                                                                                                                        |                                                                                                                                      
atGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACgcgc                                                                                                                                     >  1:513175/1‑140 (MQ=255)
  gTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACgcgc                                                                                                                                     <  2:45080/138‑1 (MQ=255)
  gTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACgcgc                                                                                                                                     >  1:45080/1‑138 (MQ=255)
      aTTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAAc                                                                                                                               >  1:639965/1‑140 (MQ=255)
        ttCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCa                                                                                                                             >  1:1169291/1‑140 (MQ=255)
         tCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCAt                                                                                                                            >  2:714903/1‑140 (MQ=255)
          cACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATg                                                                                                                           >  1:478186/1‑140 (MQ=255)
           aCCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCa                                                                                                                             >  2:148960/1‑137 (MQ=255)
           aCCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCa                                                                                                                             <  1:148960/137‑1 (MQ=255)
           aCCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGc                                                                                                                          >  1:291657/1‑140 (MQ=255)
            ccGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAAc                                                                                                                               <  2:109303/134‑1 (MQ=255)
            ccGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAAc                                                                                                                               >  1:109303/1‑134 (MQ=255)
                aGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGAcc                                                                                                                     >  2:216694/1‑140 (MQ=255)
                  ctctGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGc                                                                                                                   >  1:435166/1‑140 (MQ=255)
                     tGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCAc                                                                                                                >  1:1113621/1‑140 (MQ=255)
                        ttAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGt                                                                                                             <  2:235753/140‑1 (MQ=255)
                            acaacCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGc                                                                                                                   >  1:540706/1‑130 (MQ=255)
                            acaacCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGc                                                                                                                   <  2:540706/130‑1 (MQ=255)
                            acaacCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTaaa                                                                                                         >  2:357860/1‑140 (MQ=255)
                             caacCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTaaaa                                                                                                        <  2:1121621/140‑1 (MQ=255)
                               acCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAg                                                                                                      <  2:676926/140‑1 (MQ=255)
                               acCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAg                                                                                                      >  2:355024/1‑140 (MQ=255)
                                 ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGAc                                                                                                    >  2:112166/1‑140 (MQ=255)
                                 ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGAc                                                                                                    <  2:590690/140‑1 (MQ=255)
                                 ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGAc                                                                                                    >  1:1010803/1‑140 (MQ=255)
                                 ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGAc                                                                                                    >  2:201631/1‑140 (MQ=255)
                                 ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGAc                                                                                                    >  1:941519/1‑140 (MQ=255)
                                  cTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACa                                                                                                   >  2:73283/1‑140 (MQ=255)
                                     tGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTg                                                                                                >  1:723360/1‑140 (MQ=255)
                                        aaaTCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATc                                                                                             <  2:984188/140‑1 (MQ=255)
                                               gATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCgaga                                                                                      <  1:134314/140‑1 (MQ=255)
                                                   gAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAacac                                                                                  <  2:639965/140‑1 (MQ=255)
                                                    aaaTGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAAcacc                                                                                 <  2:983679/140‑1 (MQ=255)
                                                     aaTGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAg                                                                                                  <  1:642299/122‑1 (MQ=255)
                                                     aaTGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAg                                                                                                  >  2:642299/1‑122 (MQ=255)
                                                     aaTGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAAcacca                                                                                <  2:1020585/140‑1 (MQ=255)
                                                          aCGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTc                                                                           <  2:993294/140‑1 (MQ=255)
                                                          aCGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTc                                                                           <  2:1010803/140‑1 (MQ=255)
                                                            gATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCa                                                                         <  2:291657/140‑1 (MQ=255)
                                                                    cgcgTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGaaaa                                                                 >  1:237981/1‑140 (MQ=255)
                                                                     gcgTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAAt                                                                >  1:249402/1‑140 (MQ=255)
                                                                          gggCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGa                                                           <  1:283363/140‑1 (MQ=255)
                                                                           ggCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAg                                                          >  1:680834/1‑140 (MQ=255)
                                                                             cAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCg                                                            <  2:444396/136‑1 (MQ=255)
                                                                             cAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCg                                                            >  1:444396/1‑136 (MQ=255)
                                                                              aGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGaa                                                       <  1:73283/140‑1 (MQ=255)
                                                                               gAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAAc                                                      <  2:633831/140‑1 (MQ=255)
                                                                                     gcagGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTc                                                >  2:567346/1‑140 (MQ=255)
                                                                                      cagGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCt                                               <  1:714903/140‑1 (MQ=255)
                                                                                       agGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTg                                              <  1:1176262/140‑1 (MQ=255)
                                                                                            gcgATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGc                                         >  2:906315/1‑140 (MQ=255)
                                                                                              gATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCt                                       <  2:1113621/140‑1 (MQ=255)
                                                                                              gATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCt                                       >  1:1134321/1‑140 (MQ=255)
                                                                                                 aGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTtcg                                    <  2:205972/140‑1 (MQ=255)
                                                                                                  gACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTtcgt                                   <  2:941519/140‑1 (MQ=255)
                                                                                                         ttGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTaaa                            <  1:112166/140‑1 (MQ=255)
                                                                                                          tGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAg                           >  2:930461/1‑140 (MQ=255)
                                                                                                          tGCCGCGCAACGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAg                           >  2:1172352/1‑140 (MQ=255)
                                                                                                                 cAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGATTCTGAATGCCTTCGTCGGTAAAGAcgctgt                    >  2:830025/1‑137 (MQ=255)
                                                                                                                  aaCGCTTCCCACTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGt                   >  1:297643/1‑140 (MQ=255)
                                                                                                                            aCTGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTc         <  2:237981/140‑1 (MQ=255)
                                                                                                                              tGCACCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCa       <  1:201631/140‑1 (MQ=255)
                                                                                                                                 aCCAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGcc    >  1:5772/1‑140 (MQ=255)
                                                                                                                                   cAGACGCGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCgg  <  2:1169291/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAGACGCGGGTAAAGTTTTTCCAGCCGG  >  NC_000913/4244030‑4244300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑