Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,285,800	A→G	G524G (GGT→GGC)	acs ←	acetyl‑CoA synthetase (AMP‑forming)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,800	0	A	G	100.0%	190.8 / NA	55	G524G (GGT→GGC)	acs	acetyl‑CoA synthetase (AMP‑forming)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

CTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGAT  >  NC_000913/4285665‑4285933                                                                                                                                        |                                                                                                                                      ctccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCgg                                                                                                                                   <  1:802272/140‑1 (MQ=255)   ccccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGgag                                                                                                                                 <  2:948073/140‑1 (MQ=255)       gTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGt                                                                                                                             >  1:500713/1‑140 (MQ=255)        tGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGtt                                                                                                                            >  2:889483/1‑140 (MQ=255)          aTTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGgag                                                                                                                                 <  2:371635/133‑1 (MQ=255)          aTTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGgag                                                                                                                                 >  1:371635/1‑133 (MQ=255)          aTTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCa                                                                                                                          >  2:637931/1‑140 (MQ=255)            tAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGc                                                                                                                        <  1:889483/140‑1 (MQ=255)                        aGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCcaca                                                                                                            <  2:1178811/140‑1 (MQ=255)                            gTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGcc                                                                                                        <  2:644652/140‑1 (MQ=255)                             tAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGccc                                                                                                       >  1:630281/1‑140 (MQ=255)                             tAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGccc                                                                                                       <  2:678010/140‑1 (MQ=255)                             tAGATCGCCTGACCTTCAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGccc                                                                                                       <  2:1089425/140‑1 (MQ=255)                              aGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCg                                                                                                      <  2:1191023/140‑1 (MQ=255)                               gATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCacac                                                                                                           >  2:1034452/1‑134 (MQ=255)                               gATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCacac                                                                                                           <  1:1034452/134‑1 (MQ=255)                               gATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCACCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCgg                                                                                                     <  1:568231/140‑1 (MQ=255)                                  cGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTa                                                                                                  <  2:669405/140‑1 (MQ=255)                                    ccTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATc                                                                                                <  2:630281/140‑1 (MQ=255)                                    ccTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATc                                                                                                >  1:348704/1‑140 (MQ=255)                                       gACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAg                                                                                             <  1:637931/140‑1 (MQ=255)                                                   tgtgCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAg                                                                                             <  1:11274/128‑1 (MQ=255)                                                   tgtgCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAg                                                                                             >  2:11274/1‑128 (MQ=255)                                                    gtgCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATc                                                                                                >  2:402174/1‑124 (MQ=255)                                                    gtgCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATc                                                                                                <  1:402174/124‑1 (MQ=255)                                                     tgCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTc                                                                               >  2:536201/1‑140 (MQ=255)                                                     tgCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTc                                                                               >  1:1140825/1‑140 (MQ=255)                                                       cGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCAt                                                                             >  1:471050/1‑140 (MQ=255)                                                          aaTACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATcgc                                                                          <  1:87923/140‑1 (MQ=255)                                                               ctactaCGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCCGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGAagc                                                                     >  2:1036481/1‑140 (MQ=255)                                                                    acGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGcc                                                                >  1:204954/1‑140 (MQ=255)                                                                        cggcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTcg                                                            >  2:954006/1‑140 (MQ=255)                                                                        cggcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTcg                                                            >  2:1191044/1‑140 (MQ=255)                                                                        cggcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTcg                                                            >  1:1104555/1‑140 (MQ=255)                                                                        cggcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTAAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTcg                                                            >  2:805879/1‑140 (MQ=255)                                                                          gcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTcgcc                                                          <  1:1191044/140‑1 (MQ=255)                                                                               ggCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGa                                                     >  1:1208302/1‑140 (MQ=255)                                                                                gCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCt                                                       >  2:691791/1‑137 (MQ=255)                                                                                gCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCt                                                       <  1:691791/137‑1 (MQ=255)                                                                                   aTCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATa                                                 <  2:1140825/140‑1 (MQ=255)                                                                                        cGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACatat                                            >  1:235101/1‑140 (MQ=255)                                                                                         ggATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATAtt                                           >  1:827743/1‑140 (MQ=255)                                                                                              cgcCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGa                                      >  1:1014664/1‑140 (MQ=255)                                                                                              cgcCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGa                                      <  1:954006/140‑1 (MQ=255)                                                                                               gcCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGaa                                     <  2:235101/140‑1 (MQ=255)                                                                                                    caGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTgg                                <  1:677222/140‑1 (MQ=255)                                                                                                    caGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTgg                                <  2:874295/140‑1 (MQ=255)                                                                                                       cgcCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGgaga                             >  2:563069/1‑140 (MQ=255)                                                                                                        gcCGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGaa                            <  2:1208302/140‑1 (MQ=255)                                                                                                         ccGACTCAATCTCTGCCGTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAg                           <  2:204954/140‑1 (MQ=255)                                                                                                                          gTCCCCAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACg          <  2:827743/140‑1 (MQ=255)                                                                                                                             cccAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTc       >  2:706018/1‑140 (MQ=255)                                                                                                                               cAGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGt     <  1:563069/140‑1 (MQ=255)                                                                                                                                aGACGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTg    >  2:515471/1‑140 (MQ=255)                                                                                                                                  aCGGTGGCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGAt  >  1:827237/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGAT  >  NC_000913/4285665‑4285933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑