Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,324,212	T→C	K47K (AAA→AAG)	phnD ←	phosphonate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,324,212	0	T	C	100.0%	247.9 / NA	69	K47K (AAA→AAG)	phnD	phosphonate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/24);  total (45/24)

GATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGTTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCGAGGCAATTATCTTAGCG  >  NC_000913/4324076‑4324347                                                                                                                                         |                                                                                                                                        gATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTc                                                                                                                                      <  1:797787/140‑1 (MQ=255)             aGGTGATATCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCtgt                                                                                                                          >  2:278281/1‑140 (MQ=255)              ggCGATATCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGctt                                                                                                                                       >  2:1193264/1‑126 (MQ=255)              ggCGATATCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCtgtg                                                                                                                         >  1:1112107/1‑140 (MQ=255)              ggCGATATCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCtgtg                                                                                                                         >  1:319361/1‑140 (MQ=255)                cGATATCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGAt                                                                                                                       >  2:1115983/1‑140 (MQ=255)                  atatCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTc                                                                                                                     <  2:1167009/140‑1 (MQ=255)                   tatCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTcc                                                                                                                    >  2:648990/1‑140 (MQ=255)                     tCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGt                                                                                                                                  <  2:214952/124‑1 (MQ=255)                     tCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGt                                                                                                                                  >  1:214952/1‑124 (MQ=255)                     tCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGAt                                                                                                                       <  2:156027/135‑1 (MQ=255)                     tCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGAt                                                                                                                       >  1:156027/1‑135 (MQ=255)                             aTTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAaataa                                                                                                          >  2:605792/1‑140 (MQ=255)                                   gcgcATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCg                                                                                                     <  1:415460/139‑1 (MQ=255)                                   gcgcATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCg                                                                                                     >  2:415460/1‑139 (MQ=255)                                   gcgcATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGa                                                                                                    >  2:529981/1‑140 (MQ=255)                                   gcgcATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGa                                                                                                    >  1:576334/1‑140 (MQ=255)                                   gcgcATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGa                                                                                                    <  1:605792/140‑1 (MQ=255)                                      cATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAGAt                                                                                                 >  1:606402/1‑140 (MQ=255)                                      cATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAAt                                                                                                 >  1:233141/1‑140 (MQ=255)                                        tCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCg                                                                                                     <  2:834638/134‑1 (MQ=255)                                        tCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCg                                                                                                     >  1:834638/1‑134 (MQ=255)                                            cTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACg                                                                                           <  1:880331/140‑1 (MQ=255)                                                   aTGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTc                                                                                    >  1:1112709/1‑140 (MQ=255)                                                   aTGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTc                                                                                    <  1:278281/140‑1 (MQ=255)                                                    tGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTcc                                                                                   >  2:1052814/1‑140 (MQ=255)                                                    tGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTcc                                                                                   >  2:228913/1‑140 (MQ=255)                                                    tGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTcc                                                                                   >  1:608312/1‑140 (MQ=255)                                                    tGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTcc                                                                                   >  1:238826/1‑140 (MQ=255)                                                    tGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTcc                                                                                   >  1:309141/1‑140 (MQ=255)                                                     gCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCt                                                                                  >  1:333406/1‑140 (MQ=255)                                                      cccGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTg                                                                                 >  1:963390/1‑140 (MQ=255)                                                                   gggCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTgc                                                                    >  2:440962/1‑140 (MQ=255)                                                                    ggCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTgcg                                                                   <  2:319361/140‑1 (MQ=255)                                                                     gCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTgcgc                                                                  >  2:912774/1‑140 (MQ=255)                                                                      cAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGcc                                                                 <  1:912774/140‑1 (MQ=255)                                                                         agaagGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCggg                                                              >  1:198465/1‑140 (MQ=255)                                                                         agaagGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCggg                                                              >  2:644193/1‑140 (MQ=255)                                                                             ggCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTa                                                          <  2:198465/140‑1 (MQ=255)                                                                                gTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACa                                                       <  2:478261/140‑1 (MQ=255)                                                                                gTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACa                                                       <  2:1112709/140‑1 (MQ=255)                                                                                 ttGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAg                                                      <  1:228913/140‑1 (MQ=255)                                                                                        tCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTg                                               >  2:1201948/1‑140 (MQ=255)                                                                                              ccAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTg                                         >  2:94584/1‑140 (MQ=255)                                                                                                     cttctCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAGATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGc                                  <  2:606402/140‑1 (MQ=255)                                                                                                      ttctCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCt                                 >  2:948711/1‑140 (MQ=255)                                                                                                      ttctCCATATCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCt                                 >  1:327786/1‑140 (MQ=255)                                                                                                             tatCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAg                          <  2:502283/140‑1 (MQ=255)                                                                                                             tatCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAg                          >  2:702533/1‑140 (MQ=255)                                                                                                             tatCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAg                          >  2:1098187/1‑140 (MQ=255)                                                                                                               tCCTGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGc                        >  2:829347/1‑140 (MQ=255)                                                                                                                  tGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAg                     >  1:863865/1‑140 (MQ=255)                                                                                                                  tGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAg                     >  2:1130707/1‑140 (MQ=255)                                                                                                                  tGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAg                     <  1:1098187/140‑1 (MQ=255)                                                                                                                  tGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAg                     >  2:856488/1‑140 (MQ=255)                                                                                                                  tGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAg                     >  1:670878/1‑140 (MQ=255)                                                                                                                  tGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAg                     >  2:46573/1‑140 (MQ=255)                                                                                                                  tGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAg                     >  2:380970/1‑140 (MQ=255)                                                                                                                  tGCAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGCCTAACCAGGGGCCTGAGCCTGACCATCCTGGGGAAGCCCac                     >  1:632280/1‑139 (MQ=255)                                                                                                                    cAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCg                   >  2:364642/1‑140 (MQ=255)                                                                                                                    cAAGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCg                   <  2:933566/140‑1 (MQ=255)                                                                                                                     aaGAACGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCGa                  >  2:528672/1‑140 (MQ=255)                                                                                                                        aaCGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCGAGGc               <  1:644193/140‑1 (MQ=255)                                                                                                                         aCGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCg                   >  2:312520/1‑135 (MQ=255)                                                                                                                         aCGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCg                   <  1:312520/135‑1 (MQ=255)                                                                                                                          cGGCGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCGAGGCaa             <  1:1130707/140‑1 (MQ=255)                                                                                                                             cGTCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCGAGGCAATTa          >  2:228996/1‑140 (MQ=255)                                                                                                                               tCCATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTT‑CTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCGAGGCAATTATCt       >  1:470760/1‑140 (MQ=255)                                                                                                                                ccATTGCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCGAGGCAATTATCt       <  1:440962/140‑1 (MQ=255)                                                                                                                                     gCGGCTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCGAGGCAATTATCTTAGCg  <  1:1201948/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GATTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATCCCCTGGATAATGCCCGCGTAGTCCGGGGCAAAGAAGGCGTTGACCTTCACGCCCAGCTTCTTCTCCATATCCTGCAAGAACGGCGTCCATTGCGGTTTCAGGTTTTGCTGTGATTCCGTTGAAATAATGCCGAAATTCAACGCCTTTTCCTGCTCTTCGGCGTGCGCCGGGCTTAACAGGGTGCTGAGGCTGAACATGCTGGTGAAGGCCAGCGAGGCAATTATCTTAGCG  >  NC_000913/4324076‑4324347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑