| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,327,860 | A→G | E242E (GAA→GAG) | crfC → | clamp‑binding sister replication fork colocalization protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,327,860 | 0 | A | G | 97.8% | 148.8 / ‑4.5 | 45 | E242E (GAA→GAG) | crfC | clamp‑binding sister replication fork colocalization protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (21/23); total (22/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
TGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTT > NC_000913/4327724‑4327984 | tGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGcc > 2:226945/1‑140 (MQ=255) tCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATCCCCCCGGGCCAAATGAGGc < 2:625471/133‑1 (MQ=255) tCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGc > 1:625471/1‑133 (MQ=255) tCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCaa > 2:219660/1‑140 (MQ=255) tCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCaa > 1:98388/1‑140 (MQ=255) tCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAAc < 1:1016740/134‑1 (MQ=255) tCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAAc > 2:1016740/1‑134 (MQ=255) cTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTg < 2:98388/140‑1 (MQ=255) tCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCaaaa < 1:1031426/140‑1 (MQ=255) tCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCaaaa > 1:342805/1‑140 (MQ=255) cgccATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAAcc < 1:219660/140‑1 (MQ=255) cATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACcagc > 1:386980/1‑140 (MQ=255) aTTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACcagca > 2:317387/1‑140 (MQ=255) aGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTg > 2:283969/1‑140 (MQ=255) aGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTg > 1:1112187/1‑140 (MQ=255) atatTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCa < 2:912968/140‑1 (MQ=255) atatTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATACCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCa < 2:1058320/140‑1 (MQ=255) cccGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACgcgc < 2:1182709/140‑1 (MQ=255) tGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcg > 1:698173/1‑140 (MQ=255) tGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcg > 1:117889/1‑140 (MQ=255) tGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcg > 1:656469/1‑140 (MQ=255) gATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcgg < 1:226945/140‑1 (MQ=255) gATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcgg < 2:689820/140‑1 (MQ=255) aTTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCagcagc < 2:375073/124‑1 (MQ=255) aTTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCagcagc > 1:375073/1‑124 (MQ=255) tGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTcggcgg > 2:882547/1‑140 (MQ=255) gTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACt < 2:117889/140‑1 (MQ=255) ggAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTgg < 1:317387/140‑1 (MQ=255) gAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGc > 1:354932/1‑140 (MQ=255) tttGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGt > 1:1177903/1‑140 (MQ=255) ttGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTg < 2:698173/140‑1 (MQ=255) gTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCt > 1:27898/1‑140 (MQ=255) gTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCt < 2:656469/140‑1 (MQ=255) tCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGAGGTGGAtt > 2:321621/1‑140 (MQ=255) aGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATtata < 2:1177903/140‑1 (MQ=255) ggCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCaa < 2:354932/140‑1 (MQ=255) cGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCg < 1:126365/140‑1 (MQ=255) gTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGGTTATAAGCAAATGAAAAAGAACTcccct > 1:862930/1‑137 (MQ=255) gTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGGGCTGGGGTTTAAGCCACTGGAAAAGATCTCCgct > 2:341508/1‑138 (MQ=255) gTCAGTCGACGTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGAt < 1:829524/140‑1 (MQ=255) aGTTGACGTTACTGGATCCCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTAGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGaa < 2:108797/140‑1 (MQ=255) gTTACTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTcc < 1:38311/140‑1 (MQ=255) cTGGATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGa < 2:27898/140‑1 (MQ=255) ggATACCCCCGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAgg < 1:42051/140‑1 (MQ=255) ccccGGGCCAAATGAGGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGGGCTGGGTTATAAGCAACTGAAAAAGATCTCCCATGAAAAGGGCCGGGAggggggtt > 1:949714/1‑134 (MQ=255) | TGGACGTCGATTTTCCTTTTTCTGCTTACGCCGCCATTGAGCATATTCCCGTGATTGAAGTGGAGTTTGTCCATCTAGCGGGGCTGGAGAGTTATCCCGGTCAGTTGACGTTACTGGATACCCCCGGGCCAAATGAAGCCGGGCAACCGCATCTGCAAAAAATGCTTAACCAGCAGCTGGCACGCGCCTCGGCGGTACTGGCGGTGCTGGATTATACGCAACTGAAATCGATCTCCGATGAAGAGGTCCGTGAGGCGATTT > NC_000913/4327724‑4327984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |