Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,364,243	A→G	L267P (CTC→CCC)	dsbD ←	protein disulfide oxidoreductase ‑ DsbDreduced

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,364,243	0	A	G	100.0%	186.0 / NA	53	L267P (CTC→CCC)	dsbD	protein disulfide oxidoreductase ‑ DsbDreduced
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/28);  total (25/28)

ATCGCGCTAAGCGGTGCGGTGGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGAGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTGGAACTGTAACCCTGCGGCGGCAACCACCAGACCCAGC  >  NC_000913/4364108‑4364374                                                                                                                                        |                                                                                                                                    aTCGCGCTAAGCGGTGCGGTGGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTg                                                                                                                                 <  2:539662/140‑1 (MQ=255)  tCGCGCTAAGCGGTGCGGTGGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGc                                                                                                                                >  1:615739/1‑140 (MQ=255)  tCGCGCTAAGCGGTGCGGTGGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGc                                                                                                                                >  1:1211367/1‑140 (MQ=255)  tCGCGCTAAGCGGTGCGGTGGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGc                                                                                                                                >  1:527924/1‑140 (MQ=255)     cgcTAAGCGGTGCGGTGGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAgg                                                                                                                             >  1:388989/1‑140 (MQ=255)          aGCGGTGCGGTGGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTaaa                                                                                                                        >  1:579624/1‑140 (MQ=255)           gcggtgcggtGGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAAc                                                                                                                       <  1:1009108/140‑1 (MQ=255)           gcggtgcggtGGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAAc                                                                                                                       <  1:1107886/140‑1 (MQ=255)           gcggtgcggtGGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAAc                                                                                                                       <  1:807363/140‑1 (MQ=255)               tgcggtgGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGc                                                                                                                   <  1:623203/140‑1 (MQ=255)               tgcggtgGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGc                                                                                                                   <  2:615739/140‑1 (MQ=255)               tgcggtgGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGc                                                                                                                   <  2:579624/140‑1 (MQ=255)               tgcggtgGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGc                                                                                                                   <  2:539693/140‑1 (MQ=255)               tgcggtgGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGc                                                                                                                   <  2:527924/140‑1 (MQ=255)               ggcggtgGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGc                                                                                                                   <  2:388989/139‑1 (MQ=255)                 cggtgGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCa                                                                                                                 <  1:722604/140‑1 (MQ=255)                      gtgCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGCTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACAt                                                                                                            >  1:1096476/1‑140 (MQ=255)                              tGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCg                                                                                                    <  2:45907/140‑1 (MQ=255)                              tGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCg                                                                                                    >  1:45907/1‑140 (MQ=255)                                aaCAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGcc                                                                                                  >  2:1123336/1‑140 (MQ=255)                                   aGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGc                                                                                               <  2:47796/140‑1 (MQ=255)                                     aTCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCaa                                                                                             >  1:942524/1‑140 (MQ=255)                                        aGTCCGGCAATCGCCCCCATAACAAACGCGCCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGt                                                                                          <  2:695509/140‑1 (MQ=255)                                        aGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGt                                                                                          >  2:1116588/1‑140 (MQ=255)                                          tCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTaa                                                                                        >  1:349785/1‑140 (MQ=255)                                               cAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACg                                                                                   <  1:1088990/140‑1 (MQ=255)                                               cAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACg                                                                                   >  1:161184/1‑140 (MQ=255)                                               cAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACg                                                                                   <  2:563316/140‑1 (MQ=255)                                               cAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACg                                                                                   >  1:563316/1‑140 (MQ=255)                                                 aTCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGAt                                                                                 >  2:692565/1‑140 (MQ=255)                                                           tAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCa                                                                       <  2:598860/140‑1 (MQ=255)                                                               aaacaCACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGa                                                                   >  2:1093411/1‑140 (MQ=255)                                                               aaacaCACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGa                                                                   <  2:1211367/140‑1 (MQ=255)                                                                aacaCACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAg                                                                  <  2:187596/140‑1 (MQ=255)                                                                   acacCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCAc                                                               >  2:497249/1‑140 (MQ=255)                                                                    cacCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGa                                                                   >  2:739588/1‑135 (MQ=255)                                                                    cacCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGa                                                                   <  1:739588/135‑1 (MQ=255)                                                                     acCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGt                                                             <  1:1123336/140‑1 (MQ=255)                                                                      ccGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTa                                                            >  2:732577/1‑140 (MQ=255)                                                                       cGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTAt                                                           <  1:601252/140‑1 (MQ=255)                                                                             gTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGGTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGc                                                     >  1:897998/1‑140 (MQ=255)                                                                                       cTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGcgccg                                           >  1:58143/1‑140 (MQ=255)                                                                                            ggCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTgg                                      <  2:1096476/140‑1 (MQ=255)                                                                                            ggCGATTGCTCATCAACGTGAGACGTGGTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTgg                                      <  2:897998/140‑1 (MQ=255)                                                                                                    cTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTgg                                      >  2:109026/1‑132 (MQ=255)                                                                                                    cTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTgg                                      <  1:109026/132‑1 (MQ=255)                                                                                                    cTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTGGAACTGTaa                              >  2:452802/1‑140 (MQ=255)                                                                                                         caACGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTGGAACTGTAACCCTg                         <  2:1082945/140‑1 (MQ=255)                                                                                                            cGTGAGACGTGTTTGCAGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGGCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTGGAACTGTAACCCTgcgg                      >  2:1084775/1‑140 (MQ=255)                                                                                                                            aGCGAAGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTGGAACTGTAACCCTGCGGCGGCAACCACCAGAcc      <  2:942524/140‑1 (MQ=255)                                                                                                                                aaGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTGGAACTGTAACCCTGCGGCGGCAACCACCAGAcc      >  2:780507/1‑136 (MQ=255)                                                                                                                                aaGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTGGAACTGTAACCCTGCGGCGGCAACCACCAGAcc      <  1:780507/136‑1 (MQ=255)                                                                                                                                aaGAGGGGGGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTGGAACTGTAACCCTGCGGCGGCAACCACCAGACCCAgc  >  2:1029143/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    ATCGCGCTAAGCGGTGCGGTGGTGCATGGTGAACAGATCAGTCCGGCAATCGCCCCCATAACAAACACACCGCCAGGTGAGCCGCCCTGTTGGCGATTGCTCATCAACGTGAGACGTGTTTGCAGCGAAGAGGGGAGTTGCAGGGTAAACAAGCCAAACATTGACATCGCCAGCAAGGTAAAGACGATGGCGAGGCCAATGAGCACGTATGGGTGCTGTAGCGCCGCCTGGAACTGTAACCCTGCGGCGGCAACCACCAGACCCAGC  >  NC_000913/4364108‑4364374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑