Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,437,277	A→G	T175A (ACC→GCC)	cysQ →	3'(2'),5'‑bisphosphate nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,437,277	0	A	G	100.0%	221.2 / NA	64	T175A (ACC→GCC)	cysQ	3'(2'),5'‑bisphosphate nucleotidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/30);  total (34/30)

CAAAGCGTGGAAAGAAGAGTGCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGT  >  NC_000913/4437141‑4437413                                                                                                                                         |                                                                                                                                         cAAAGCGTGGAAAGAAGAGTGCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCa                                                                                                                                       <  2:682526/140‑1 (MQ=255) aaaggCGTGGAAAGAAGGGTGCGGTGTGCCCAAGCAGATTCAGGTCCGCGAGGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCa                                                                                                                                       <  2:138749/136‑1 (MQ=255)    aGCGTGGAAAGAAGAGTGCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGt                                                                                                                                    >  2:107857/1‑140 (MQ=255)         ggAAAGAAGAGTGCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATc                                                                                                                               >  2:1054144/1‑140 (MQ=255)          gAAAGAAGAGTGCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCg                                                                                                                              >  1:138749/1‑140 (MQ=255)          gAAAGAAGAGTGCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCg                                                                                                                              >  1:673810/1‑140 (MQ=255)             agaagaGTGCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGct                                                                                                                           <  1:549167/140‑1 (MQ=255)              gaagaGTGCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGctc                                                                                                                          <  2:897081/140‑1 (MQ=255)                       ggTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGaa                                                                                                                 >  1:1085565/1‑140 (MQ=255)                                   cAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCtggt                                                                                                     >  2:790700/1‑140 (MQ=255)                                     gATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTg                                                                                                                   <  1:1139469/124‑1 (MQ=255)                                     gATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTg                                                                                                                   >  2:1139469/1‑124 (MQ=255)                                      aTTCAGGTCCGGGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGc                                                                                                  <  1:847357/140‑1 (MQ=255)                                      aTTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGc                                                                                                  <  2:673810/140‑1 (MQ=255)                                         cAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGa                                                                                               <  1:107857/140‑1 (MQ=255)                                              ccGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGAc                                                                                          <  1:3271/140‑1 (MQ=255)                                                gcgATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAg                                                                                        >  2:958821/1‑140 (MQ=255)                                                gcgATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAg                                                                                        >  1:567987/1‑140 (MQ=255)                                                gcgATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAg                                                                                        >  1:167886/1‑140 (MQ=255)                                                gcgATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAg                                                                                        >  1:991455/1‑140 (MQ=255)                                                  gATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGgc                                                                                      <  2:330646/140‑1 (MQ=255)                                                         gcccgccGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGNTGGCGGAAGGACAGGCGCAGCTg                                                                               <  2:1085565/140‑1 (MQ=255)                                                              ccgcTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTAccc                                                                          >  1:752020/1‑140 (MQ=255)                                                              ccgcTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTAccc                                                                          >  1:1088107/1‑140 (MQ=255)                                                              ccgcTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTAccc                                                                          <  1:1054144/140‑1 (MQ=255)                                                                        tgATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGa                                                                <  1:763833/140‑1 (MQ=255)                                                                          aTCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGAcc                                                              >  1:83723/1‑140 (MQ=255)                                                                          aTCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGAcc                                                              >  2:6223/1‑140 (MQ=255)                                                                              gCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACg                                                          >  2:44195/1‑140 (MQ=255)                                                                              gCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACg                                                          >  2:258316/1‑140 (MQ=255)                                                                                    cccATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATAtt                                                    >  2:1071764/1‑140 (MQ=255)                                                                                         gcggatgcggaGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGa                                               <  1:790700/140‑1 (MQ=255)                                                                                            gatgcggaGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGacac                                            <  1:457391/140‑1 (MQ=255)                                                                                            gatgcggaGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGacac                                            >  1:546609/1‑140 (MQ=255)                                                                                             atgcggaGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACAcc                                           >  1:718505/1‑140 (MQ=255)                                                                                              tgcggaGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACAccg                                          <  2:991455/140‑1 (MQ=255)                                                                                                cggaGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACAccgcc                                        >  2:576222/1‑140 (MQ=255)                                                                                                  gaGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACcgccgc                                      >  2:313457/1‑140 (MQ=255)                                                                                                   aGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCt                                     >  1:472743/1‑140 (MQ=255)                                                                                                   aGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCt                                     <  1:6223/140‑1 (MQ=255)                                                                                                   aGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCt                                     <  2:546609/140‑1 (MQ=255)                                                                                                   aGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCt                                     <  1:465304/140‑1 (MQ=255)                                                                                                       gAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGAc                                 >  2:76420/1‑140 (MQ=255)                                                                                                       gAAAGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGAc                                 <  2:304292/140‑1 (MQ=255)                                                                                                        aaaGAGTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACa                                <  2:752020/140‑1 (MQ=255)                                                                                                          agagTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATg                              >  2:147052/1‑140 (MQ=255)                                                                                                             gTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACAccgcc                                        <  2:221126/127‑1 (MQ=255)                                                                                                             gTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACAccgcc                                        >  1:221126/1‑127 (MQ=255)                                                                                                             gTATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTg                           >  1:595843/1‑140 (MQ=255)                                                                                                                tCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGa                                  >  2:535181/1‑130 (MQ=255)                                                                                                                tCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGa                                  <  1:535181/130‑1 (MQ=255)                                                                                                                    cAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTagctgc                    <  1:313457/140‑1 (MQ=255)                                                                                                                      aCAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTagctgcag                  >  1:627046/1‑140 (MQ=255)                                                                                                                          cTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTagctgcagctgc              >  1:1191610/1‑140 (MQ=255)                                                                                                                          cTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTagctgcagctgc              >  2:23138/1‑140 (MQ=255)                                                                                                                            tGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCg            >  2:596052/1‑140 (MQ=255)                                                                                                                             ggCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCgg           >  1:746822/1‑140 (MQ=255)                                                                                                                               cGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAg         <  1:983043/140‑1 (MQ=255)                                                                                                                                 aaCATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAgcg       <  2:201001/140‑1 (MQ=255)                                                                                                                                  aCATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAgcgc      <  2:627046/140‑1 (MQ=255)                                                                                                                                  aCATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAgcgc      <  1:147052/140‑1 (MQ=255)                                                                                                                                  aCATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAgcgc      <  2:1191610/140‑1 (MQ=255)                                                                                                                                    aTCAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCAc    >  2:181047/1‑140 (MQ=255)                                                                                                                                      cAGGCCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGt  <  2:279141/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CAAAGCGTGGAAAGAAGAGTGCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGT  >  NC_000913/4437141‑4437413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑