Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,461,803	A→G	G286G (GGT→GGC)	nrdD ←	anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,461,803	0	A	G	100.0%	228.5 / NA	65	G286G (GGT→GGC)	nrdD	anaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/38);  total (27/38)

CACCAGTTTCGGGAACACCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGATGTTTG  >  NC_000913/4461668‑4461938                                                                                                                                         |                                                                                                                                        cacCAGTTTCGGGAACACCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGt                                                                                                                                     <  1:125069/140‑1 (MQ=255)     aGTTTCGGGAACACCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGc                                                                                                                                 <  2:180723/140‑1 (MQ=255)     aGTTTCGGGAACACCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGc                                                                                                                                 <  2:322945/140‑1 (MQ=255)       tttCGGTAACACCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCgg                                                                                                                               <  2:1216414/140‑1 (MQ=255)       tttCGGGAACACCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCgg                                                                                                                               <  2:1158016/140‑1 (MQ=255)           gggAACACCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGtgtg                                                                                                                           >  2:632104/1‑140 (MQ=255)           gggAACACCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGtgtg                                                                                                                           >  2:1037697/1‑140 (MQ=255)            ggAACACCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGc                                                                                                                          <  1:605677/140‑1 (MQ=255)                cacCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAggg                                                                                                                      >  1:579927/1‑140 (MQ=255)                cacCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAg                                                                                                                        <  1:162878/138‑1 (MQ=255)                cacCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAg                                                                                                                        >  2:162878/1‑138 (MQ=255)                 acCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAATTCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCg                                                                                                                                >  2:951599/1‑130 (MQ=255)                 acCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAATTCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCg                                                                                                                                <  1:951599/130‑1 (MQ=255)                   cGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCgg                                                                                                                               >  2:313801/1‑128 (MQ=255)                   cGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCgg                                                                                                                               <  1:313801/128‑1 (MQ=255)                   cGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGgtgt                                                                                                                   >  1:971500/1‑140 (MQ=255)                   cGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGgtgt                                                                                                                   >  1:601435/1‑140 (MQ=255)                   cGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGgtgt                                                                                                                   >  1:153730/1‑140 (MQ=255)                     cAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGttt                                                                                                                 <  2:971500/140‑1 (MQ=255)                        ttttACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACt                                                                                                              <  1:37742/140‑1 (MQ=255)                        ttttACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACt                                                                                                              <  2:1059771/140‑1 (MQ=255)                           tACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCg                                                                                                           >  1:880231/1‑140 (MQ=255)                           tACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCg                                                                                                           <  1:1198399/140‑1 (MQ=255)                           tACGGCTTTTACCGAGGCCTGCGATACGGTTACGCAGGATAGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCg                                                                                                           <  2:1075705/140‑1 (MQ=255)                             cGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTa                                                                                                         >  1:481877/1‑140 (MQ=255)                                    tACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGt                                                                                                  <  1:1155472/140‑1 (MQ=255)                                       cGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGAc                                                                                               <  1:729478/140‑1 (MQ=255)                                               gCGATACGGTTACGAAGGATCGACTCCTGAA‑TCAGGCGGGATTCCCAGCTGGTGCCC‑GGCCAAACCCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCa                                                                                      <  2:469926/140‑1 (MQ=255)                                                cGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCa                                                                                      >  1:490442/1‑140 (MQ=255)                                                      ggTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAg                                                                                <  2:222387/140‑1 (MQ=255)                                                      ggTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAg                                                                                <  2:153730/140‑1 (MQ=255)                                                      ggTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAg                                                                                <  1:560054/140‑1 (MQ=255)                                                      ggTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAg                                                                                <  2:880231/140‑1 (MQ=255)                                                      ggTTACGCAGGAACGATTTCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAg                                                                                <  1:291629/140‑1 (MQ=255)                                                           cGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGTGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAgg                                                                                        >  2:176931/1‑127 (MQ=255)                                                           cGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGTGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAgg                                                                                        <  1:176931/127‑1 (MQ=255)                                                            gCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGCGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCActct                                                                          >  2:144098/1‑140 (MQ=255)                                                              aGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCttt                                                                        <  1:493690/140‑1 (MQ=255)                                                                       tCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCg                                                               <  1:1096329/140‑1 (MQ=255)                                                                          tGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGa                                                                      >  1:443227/1‑130 (MQ=255)                                                                          tGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGa                                                                      <  2:443227/130‑1 (MQ=255)                                                                          tGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCgaga                                                            >  2:135920/1‑140 (MQ=255)                                                                           gAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGagag                                                           <  1:1037697/140‑1 (MQ=255)                                                                           gAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGagag                                                           <  1:632104/140‑1 (MQ=255)                                                                                  ggCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCg                                                     <  1:144098/140‑1 (MQ=255)                                                                                  ggCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCg                                                     <  1:869644/140‑1 (MQ=255)                                                                                   gcgcGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGa                                                                      <  2:51135/122‑1 (MQ=255)                                                                                   gcgcGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGa                                                                      >  1:51135/1‑122 (MQ=255)                                                                                         ttCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCtt                                              >  1:366425/1‑140 (MQ=255)                                                                                             cAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGc                                          >  1:1024896/1‑140 (MQ=255)                                                                                               gCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGt                                        >  2:192304/1‑140 (MQ=255)                                                                                                   gTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg                                    >  1:744523/1‑140 (MQ=255)                                                                                                     gCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGa                                  >  2:215346/1‑140 (MQ=255)                                                                                                     gCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGa                                  <  1:588879/140‑1 (MQ=255)                                                                                                     gCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGa                                  >  1:369461/1‑140 (MQ=255)                                                                                                     gCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGa                                  >  2:1149292/1‑140 (MQ=255)                                                                                                          ggCCAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTc                             >  1:670865/1‑140 (MQ=255)                                                                                                            ccAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCa                           <  2:670865/140‑1 (MQ=255)                                                                                                            ccAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCa                           <  1:200845/140‑1 (MQ=255)                                                                                                            ccAAAACCAAAGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCa                           <  2:1024896/140‑1 (MQ=255)                                                                                                                      aGGTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCg                 <  2:81393/140‑1 (MQ=255)                                                                                                                        gTTACAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGt               <  2:415923/140‑1 (MQ=255)                                                                                                                           aCAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCtt            >  1:886586/1‑140 (MQ=255)                                                                                                                            cAAACGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTg           >  2:236955/1‑140 (MQ=255)                                                                                                                             aaaCGGCGTCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGc          <  2:956374/140‑1 (MQ=255)                                                                                                                                     tCTGGCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGAtgtttg  <  1:215346/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CACCAGTTTCGGGAACACCGCAGTTTTACGGTTTTTACCGAGGCCTGCGATACGGTTACGCAGGATCGATTCCTGAA‑TCAGGCGCGATTCCCAGCTGGTGCCCAGGCCAAAACCAAAGGTTACAAACGGCGTCTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGATGTTTG  >  NC_000913/4461668‑4461938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑