Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,482,624	A→G	V405A (GTA→GCA)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,482,624	0	A	G	100.0%	129.4 / NA	40	V405A (GTA→GCA)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/14);  total (26/14)

TTGCGGCCAACATAAACGTTACCCGCTTCGTCATACCATGCCGGGATACGGTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAG  >  NC_000913/4482489‑4482754                                                                                                                                        |                                                                                                                                   ttGCGGCCAACATAAACGTTACCCGCTTCGTCATACCATGCCGGGATACGGTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGaa                                                                                                                                <  2:504530/140‑1 (MQ=255)                      cccGCTTCGTCATACCATGCCGGGATACGGTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGaa                                                                                                                           <  2:149004/124‑1 (MQ=255)                      cccGCTTCGTCATACCATGCCGGGATACGGTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGaa                                                                                                                           >  1:149004/1‑124 (MQ=255)                          cTTCGTCATACCATGCCGGGATACGGTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCt                                                                                                       >  2:868195/1‑140 (MQ=255)                           ttCGTCATACCATGCCGGGATACGGTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCtt                                                                                                      >  2:28238/1‑140 (MQ=255)                                       tGCCGGGATACGGTGCCCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAAccg                                                                                                <  2:138146/134‑1 (MQ=255)                                       tGCCGGGATACGGTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAAccg                                                                                                >  1:138146/1‑134 (MQ=255)                                       tGCCGGGATACGGTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGtt                                                                                          >  2:775811/1‑140 (MQ=255)                                       tGCCGGGATACGGTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGtt                                                                                          <  1:610298/140‑1 (MQ=255)                                        gCCGGGATACGGTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGttt                                                                                         >  2:251902/1‑140 (MQ=255)                                        gCCGGGATACGGTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGttt                                                                                         >  2:606305/1‑140 (MQ=255)                                                  ggTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACa                                                                               >  2:90706/1‑140 (MQ=255)                                                  ggTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACa                                                                               >  2:203332/1‑140 (MQ=255)                                                      aCCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCgg                                                                           <  1:36843/140‑1 (MQ=255)                                                      aCCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCgg                                                                           >  1:726347/1‑140 (MQ=255)                                                      aCCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCgg                                                                           >  1:131496/1‑140 (MQ=255)                                                        cccACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGgca                                                                         <  1:203332/140‑1 (MQ=255)                                                           accacAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGgcacgc                                                                      >  2:41757/1‑140 (MQ=255)                                                            ccacAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGgcacgca                                                                     <  1:775811/140‑1 (MQ=255)                                                            ccacAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGgcacgca                                                                     <  2:111001/140‑1 (MQ=255)                                                              acaACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGcacgcacg                                                                   <  2:456053/140‑1 (MQ=255)                                                                  cTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTAcc                                                               <  2:131496/140‑1 (MQ=255)                                                                        agagaTACACCAGTCCTAAATATCGCGCACCCAGGAGAAGTACATTTTTTCGTACTGTTTCGGTGCAAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTggt                                                         <  2:425791/140‑1 (MQ=255)                                                                            aTACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTggtcggt                                                     >  1:430409/1‑140 (MQ=255)                                                                            aTACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTggtcggt                                                     >  1:908561/1‑140 (MQ=255)                                                                                   gttcttGAATATCGCGCACCGAGGAAAGGTACTTTTTTTCGTACTGTTTGGGGGCGAGGTGATTGTCGCCGTTCTCACCCGCTCCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCAtt                                              <  2:597387/135‑1 (MQ=255)                                                                                   attCCTAAATATCGCCCATCCAGGAGAAGTACATTTTTTCTTACTGCTTGGGTGCAAACTGAATGTCCCCGTTCCCACCCCCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCAtt                                              <  2:908561/138‑1 (MQ=255)                                                                                   aGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCAtt                                              <  2:969822/140‑1 (MQ=255)                                                                                   aGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCAtt                                              <  1:90706/140‑1 (MQ=255)                                                                                   aGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCAtt                                              >  1:516165/1‑140 (MQ=255)                                                                                    gTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTg                                             >  1:272691/1‑140 (MQ=255)                                                                                     tCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTgg                                            >  2:833908/1‑140 (MQ=255)                                                                                     tCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTgg                                            >  1:621316/1‑140 (MQ=255)                                                                                      ccTGAATATCGCGCATCTAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGt                                           >  1:1202164/1‑140 (MQ=255)                                                                                         gAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCg                                        <  2:272691/140‑1 (MQ=255)                                                                                          aaTATCGCGCATCTAGGGAAAGTACTTGTTTTCGTCCGGTTTGGGTGCGAGCTAATTTTCGCGGTTCAAACCCGCTCAACCCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGTATTGGTTCGa                                       <  2:1202164/140‑1 (MQ=255)                                                                                          aaTATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGa                                       >  2:647531/1‑140 (MQ=255)                                                                                               cgcgCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACt                                  >  2:264865/1‑140 (MQ=255)                                                                                                 cgcATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTAc                                >  1:1019960/1‑140 (MQ=255)                                                                                                       cAGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACgccgcc                          <  2:516165/140‑1 (MQ=255)                                                                                                        aGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTAcg                               >  1:501426/1‑134 (MQ=255)                                                                                                        aGGAGAAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTAcg                               <  2:501426/134‑1 (MQ=255)                                                                                                            gaAGTACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGt                     <  1:251902/140‑1 (MQ=255)                                                                                                              aGTACATTTTTTCGTACTGCTTCGGTGCGAACTAAATGCCGCCGTTCTCACCCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCg                   <  2:1143638/140‑1 (MQ=255)                                                                                                                tACATGTTTTCGTACTGCTTCGGTGCGAATTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGcc                 >  2:789426/1‑140 (MQ=255)                                                                                                                tACATGTTTTCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGcc                 >  2:598368/1‑140 (MQ=255)                                                                                                                      ttttCGTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAgg           <  1:647531/140‑1 (MQ=255)                                                                                                                           gTACTGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACgg      <  1:264865/140‑1 (MQ=255)                                                                                                                               tGCTTCGGTGCGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAg  >  2:655697/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   TTGCGGCCAACATAAACGTTACCCGCTTCGTCATACCATGCCGGGATACGGTGACCCCACCACAACTGACGAGAGATACACCAGTCCTGAATATCGCGCATCCAGGAGAAGTACATGTTTTCGTACTGCTTCGGTACGAACTGAATGTCGCCGTTCTCAACCGCTTCAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAG  >  NC_000913/4482489‑4482754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑