Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	497,702	A→G	A176A (GCA→GCG)	adk →	adenylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	497,702	0	A	G	100.0%	172.7 / NA	49	A176A (GCA→GCG)	adk	adenylate kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/24);  total (25/24)

GTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACG  >  NC_000913/497566‑497835                                                                                                                                         |                                                                                                                                      gtgtTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCg                                                                                                                                    <  1:1082487/140‑1 (MQ=255)    tttATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCg                                                                                                                                    >  2:543595/1‑137 (MQ=255)    tttATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCg                                                                                                                                    <  1:543595/137‑1 (MQ=255)     ttATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGa                                                                                                                                <  1:61023/140‑1 (MQ=255)      tATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGAt                                                                                                                               <  1:1025417/140‑1 (MQ=255)         cACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCgg                                                                                                                            >  2:1145765/1‑140 (MQ=255)            gTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGcta                                                                                                                         <  1:1145765/140‑1 (MQ=255)                                aGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAagaagcagaag                                                                                                     <  1:1077744/140‑1 (MQ=255)                                    gAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCggg                                                                                                 <  1:123893/140‑1 (MQ=255)                                    gAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCggg                                                                                                 <  2:1187884/140‑1 (MQ=255)                                         cAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAagaagcagaag                                                                                                     <  2:772040/131‑1 (MQ=255)                                         cAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAagaagcagaag                                                                                                     >  1:772040/1‑131 (MQ=255)                                           aaGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATAcc                                                                                          <  2:426301/140‑1 (MQ=255)                                                             aagaaCTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAACAGAAGCGGGTAATACCAAATACGCGAAAGTTGAc                                                                        <  2:632293/140‑1 (MQ=255)                                                              agaaCTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACg                                                                       >  2:954859/1‑140 (MQ=255)                                                               gaaCTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACgg                                                                      <  1:1199129/140‑1 (MQ=255)                                                                               aaGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCgg                                                           <  2:569642/135‑1 (MQ=255)                                                                               aaGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCgg                                                           >  1:569642/1‑135 (MQ=255)                                                                                 gatgatCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGa                                                    >  2:500841/1‑140 (MQ=255)                                                                                    gatCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGt                                                 >  1:931400/1‑140 (MQ=255)                                                                                     atCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGtt                                                >  2:724037/1‑140 (MQ=255)                                                                                     atCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGtt                                                >  2:646009/1‑140 (MQ=255)                                                                                         ggAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTcgcg                                            <  1:646009/140‑1 (MQ=255)                                                                                             gagaCCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGa                                        >  1:267751/1‑140 (MQ=255)                                                                                               gaCCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATc                                      >  2:942737/1‑140 (MQ=255)                                                                                                aCCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTcgcg                                            <  1:846561/133‑1 (MQ=255)                                                                                                aCCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTcgcg                                            >  2:846561/1‑133 (MQ=255)                                                                                                aCCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCt                                     >  2:1199821/1‑140 (MQ=255)                                                                                                    tacgtaAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTcgcg                                            >  1:610538/1‑129 (MQ=255)                                                                                                    tacgtaAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTcgcg                                            <  2:610538/129‑1 (MQ=255)                                                                                                    tacgtaAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGaa                                 >  2:520311/1‑140 (MQ=255)                                                                                                    tacgtaAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGaa                                 >  2:792401/1‑140 (MQ=255)                                                                                                    tacgtaAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGaa                                 >  1:452664/1‑140 (MQ=255)                                                                                                      cgtaAACGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGaaaa                               <  2:45656/140‑1 (MQ=255)                                                                                                         aaaCGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAAt                            >  2:916542/1‑140 (MQ=255)                                                                                                           aCGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATcc                          <  2:452664/140‑1 (MQ=255)                                                                                                            cGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCt                         >  1:821219/1‑140 (MQ=255)                                                                                                            cGTCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCt                         <  1:724037/140‑1 (MQ=255)                                                                                                              tCTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCg                       >  1:586736/1‑140 (MQ=255)                                                                                                               cTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCgg                      >  2:275114/1‑140 (MQ=255)                                                                                                               cTGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCgg                      >  1:428984/1‑140 (MQ=255)                                                                                                                tGGTTGAATACCATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGAACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGc                     <  2:571982/140‑1 (MQ=255)                                                                                                                           cATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCCCCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAg          <  1:500841/140‑1 (MQ=255)                                                                                                                           cATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATc                           <  1:53008/123‑1 (MQ=255)                                                                                                                           cATCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATc                           >  2:53008/1‑123 (MQ=255)                                                                                                                             tCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAgcg        <  2:528526/140‑1 (MQ=255)                                                                                                                             tCAGATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAgcg        >  1:971432/1‑140 (MQ=255)                                                                                                                                gATGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAgcgcgc     <  2:586736/140‑1 (MQ=255)                                                                                                                                 aTGACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCa    >  2:470516/1‑140 (MQ=255)                                                                                                                                   gACAGCGCCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACg  <  2:428984/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCGCTGATCTGGAAAAAATCCTCGGCTAATTCGAAAGCGCGCACG  >  NC_000913/497566‑497835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑