Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,533,126	A→G	F20L (TTC→CTC)	yjhP ←	KpLE2 phage‑like element; putative methyltransferase YjhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,533,126	0	A	G	100.0%	190.0 / NA	54	F20L (TTC→CTC)	yjhP	KpLE2 phage‑like element; putative methyltransferase YjhP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/29);  total (25/29)

ATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATC  >  NC_000913/4532990‑4533262                                                                                                                                         |                                                                                                                                         aTGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCgg                                                                                                                                       >  2:566296/1‑140 (MQ=255)   gCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGt                                                                                                                                     >  2:57365/1‑140 (MQ=255)        tCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATgg                                                                                                                                >  1:1115321/1‑140 (MQ=255)          cccGTAATGCCATGATCCCTGGTCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGAt                                                                                                                              <  1:30164/140‑1 (MQ=255)           ccGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATg                                                                                                                             <  1:178962/140‑1 (MQ=255)             gTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATg                                                                                                                             <  2:224896/138‑1 (MQ=255)             gTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATg                                                                                                                             >  1:224896/1‑138 (MQ=255)                   ccATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTc                                                                                                                     >  1:905069/1‑140 (MQ=255)                    cATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTTCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGtt                                                                                                                                    >  2:1084136/1‑124 (MQ=255)                    cATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGtt                                                                                                                                    <  1:1084136/124‑1 (MQ=255)                            cTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCt                                                                                                            <  1:335613/140‑1 (MQ=255)                              ggCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGa                                                                                                          <  1:566296/140‑1 (MQ=255)                               gCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGAt                                                                                                         <  2:381063/140‑1 (MQ=255)                                    ggTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTaa                                                                                                    >  1:1126108/1‑140 (MQ=255)                                    ggTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTaa                                                                                                    >  2:269987/1‑140 (MQ=255)                                    ggTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTaa                                                                                                    >  2:978680/1‑140 (MQ=255)                                    ggTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTaa                                                                                                    <  1:234334/140‑1 (MQ=255)                                    ggTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTaa                                                                                                    >  2:882059/1‑140 (MQ=255)                                    ggTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTaa                                                                                                    >  2:1114644/1‑140 (MQ=255)                                        cAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAAt                                                                                                >  2:633900/1‑140 (MQ=255)                                          gagCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATAc                                                                                              >  1:943725/1‑140 (MQ=255)                                           agCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACg                                                                                             <  1:1008033/140‑1 (MQ=255)                                             cATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTg                                                                                           >  2:1193648/1‑140 (MQ=255)                                             cATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTg                                                                                           <  1:269987/140‑1 (MQ=255)                                               tctcGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTggg                                                                                         <  2:349488/140‑1 (MQ=255)                                                ctcGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGTTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGa                                                                                        >  2:586363/1‑140 (MQ=255)                                                ctcGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGa                                                                                        >  2:103276/1‑140 (MQ=255)                                                 tcGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGTTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGat                                                                                       <  1:586363/140‑1 (MQ=255)                                                 tcGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGat                                                                                       <  1:1005829/140‑1 (MQ=255)                                                 tcGCCCGAGCAGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGat                                                                                       <  1:1133469/140‑1 (MQ=255)                                                      cGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCa                                                                                  <  1:633900/140‑1 (MQ=255)                                                               gCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAAt                                                                         <  2:1115321/140‑1 (MQ=255)                                                                 cGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTc                                                                       <  2:905069/140‑1 (MQ=255)                                                                  gAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGAGTTCACTTTCGCTGATGGTAAAAATACGTGGGATACCCATTATTTAATTcc                                                                      <  2:900711/140‑1 (MQ=255)                                                                   aGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCt                                                                     >  2:251231/1‑140 (MQ=255)                                                                       cAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTa                                                                 <  1:1114644/140‑1 (MQ=255)                                                                              gCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGtt                                                          <  1:781634/140‑1 (MQ=255)                                                                              gCGGGGGCTCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTGCTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGtt                                                          <  2:224784/140‑1 (MQ=255)                                                                               cGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTa                                                         >  2:1190484/1‑140 (MQ=255)                                                                               cGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTa                                                         >  1:191833/1‑140 (MQ=255)                                                                                   tGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGcc                                                     <  1:1191217/140‑1 (MQ=255)                                                                                     cccGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCtt                                                   >  2:681056/1‑140 (MQ=255)                                                                                             cATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGa                                           <  1:251231/140‑1 (MQ=255)                                                                                             cATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGa                                           <  1:1193648/140‑1 (MQ=255)                                                                                                      caCGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAg                                  >  1:310509/1‑140 (MQ=255)                                                                                                         gcgACCCAGCGTGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGAtt                               <  1:681056/140‑1 (MQ=255)                                                                                                                    tGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTATTCACGt                    <  1:882059/140‑1 (MQ=255)                                                                                                                    tGGCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTATTCACGt                    <  1:978680/140‑1 (MQ=255)                                                                                                                     ggCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTAtt                         >  1:1007560/1‑134 (MQ=255)                                                                                                                     ggCGTACTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTAtt                         <  2:1007560/134‑1 (MQ=255)                                                                                                                           cTTCTCTTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTATTCACGTCAGTGCt             >  1:398490/1‑140 (MQ=255)                                                                                                                              ctctTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTATTCACGTCAGTGCTCCg          >  1:1160931/1‑140 (MQ=255)                                                                                                                                ctTCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTATTCACGTCAGTGCTCCGGt        >  2:828728/1‑140 (MQ=255)                                                                                                                                 ttCGGTGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTATTCACGTCAGTGCTCCGGtt       <  1:828728/140‑1 (MQ=255)                                                                                                                                      tGAGCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTATTCACGTCAGTGCTCCGGTTtcatc  <  1:103276/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         ATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCGGCTTCATGCGCAGCACGCGACCCAGCGTGGCGTACTTCTCTTCGGTGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATC  >  NC_000913/4532990‑4533262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑