Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,384	A→G	D230G (GAT→GGT)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,384	0	A	G	100.0%	235.0 / NA	66	D230G (GAT→GGT)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/38);  total (28/38)

TTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATT  >  NC_000913/4637248‑4637517                                                                                                                                         |                                                                                                                                      ttATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGtct                                                                                                                                    <  2:770931/140‑1 (MQ=255)    tGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCgg                                                                                                                                 <  1:1027334/140‑1 (MQ=255)    tGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCgg                                                                                                                                 <  2:808339/140‑1 (MQ=255)         cAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGgtagta                                                                                                                            <  2:611586/140‑1 (MQ=255)                aTTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGATATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGtt                                                                                                                     <  2:263304/140‑1 (MQ=255)                         gggATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACt                                                                                                            >  1:310326/1‑140 (MQ=255)                             ttGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTcgcg                                                                                                        >  1:193506/1‑140 (MQ=255)                              tGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTGCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCgcgc                                                                                                       >  2:1135891/1‑140 (MQ=255)                              tGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCgcgc                                                                                                       <  1:494099/140‑1 (MQ=255)                                cACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAg                                                                                                     >  2:185542/1‑140 (MQ=255)                                cACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAg                                                                                                     <  2:1063119/140‑1 (MQ=255)                                 aCTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAgg                                                                                                    <  1:1089286/140‑1 (MQ=255)                                  cTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGgc                                                                                                   <  1:4803/140‑1 (MQ=255)                                    ggTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGgcgc                                                                                                 <  2:310326/140‑1 (MQ=255)                                     gTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCt                                                                                                >  1:721183/1‑140 (MQ=255)                                     gTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCt                                                                                                >  1:1093668/1‑140 (MQ=255)                                      tGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTg                                                                                               >  2:311680/1‑140 (MQ=255)                                      tGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTg                                                                                               >  1:320050/1‑140 (MQ=255)                                        aTTGGCGCAAGCATGGTTTGGGGGATCAACCGCTCTATTGCCCGGCTCAGTCGCTATGCTGATTCCGTCACTCACAATAAGCCCGTTCCTCTCCCCGGTCTCGGGAGAAGCGAGATGCGTAAACTCGCGCAGGCGCTGGa                                                                                             >  2:552822/1‑140 (MQ=255)                                          tGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCTGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGaaa                                                                                           >  1:928399/1‑140 (MQ=255)                                           ggCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAg                                                                                          <  2:193506/140‑1 (MQ=255)                                                  gCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATgcgc                                                                                   <  2:320050/140‑1 (MQ=255)                                                   cATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGcgcg                                                                                  <  2:598758/140‑1 (MQ=255)                                                      ggTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGa                                                                               <  1:311680/140‑1 (MQ=255)                                                      ggTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGa                                                                               <  2:319894/140‑1 (MQ=255)                                                            gtggATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAg                                                                             <  2:899206/136‑1 (MQ=255)                                                            gtggATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAg                                                                             >  1:899206/1‑136 (MQ=255)                                                            gtggATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTgg                                                                         >  1:100182/1‑140 (MQ=255)                                                            gtggATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTgg                                                                         <  1:331272/140‑1 (MQ=255)                                                            gtggATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCATGAAGCTgg                                                                         >  1:1161905/1‑140 (MQ=255)                                                             tggATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGa                                                                        >  1:835773/1‑140 (MQ=255)                                                                 tCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAggg                                                                    >  1:684387/1‑140 (MQ=255)                                                                 tCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAggg                                                                    <  2:666557/140‑1 (MQ=255)                                                                 tCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAggg                                                                    >  2:53884/1‑140 (MQ=255)                                                                 tCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAggg                                                                    >  2:826828/1‑140 (MQ=255)                                                                   aaCCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGaa                                                                  <  2:721183/140‑1 (MQ=255)                                                                      cGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGaaaaa                                                               <  1:1135891/140‑1 (MQ=255)                                                                      cGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGaaaaa                                                               <  2:276516/140‑1 (MQ=255)                                                                        ctctATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACt                                                             >  2:877611/1‑140 (MQ=255)                                                                          ctATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACtat                                                           >  1:422038/1‑140 (MQ=255)                                                                           tATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACtata                                                          >  2:1088800/1‑140 (MQ=255)                                                                             ttGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATAtt                                                        <  2:835773/140‑1 (MQ=255)                                                                              tGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTg                                                       <  1:185542/140‑1 (MQ=255)                                                                                cccGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCTGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAg                                                     <  2:928399/140‑1 (MQ=255)                                                                                cccGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAg                                                     <  2:1093668/140‑1 (MQ=255)                                                                                cccGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCCCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAg                                                     <  1:552822/140‑1 (MQ=255)                                                                                  cGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCa                                                   >  2:200882/1‑140 (MQ=255)                                                                                   ggCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAg                                                  <  1:902251/140‑1 (MQ=255)                                                                                          tCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCATGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGttt                                           <  2:1161905/140‑1 (MQ=255)                                                                                           cGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTa                                          <  2:609518/140‑1 (MQ=255)                                                                                              tATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGc                                       <  1:440053/140‑1 (MQ=255)                                                                                                  cTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTa                                   >  1:519490/1‑140 (MQ=255)                                                                                                    gATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAAc                                 >  1:100622/1‑140 (MQ=255)                                                                                                    gATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAAc                                 >  1:436792/1‑140 (MQ=255)                                                                                                      ttCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTc                               <  1:22042/140‑1 (MQ=255)                                                                                                                      aaGCCCGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGccc               <  2:422038/140‑1 (MQ=255)                                                                                                                          ccGTTCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTg           <  1:877611/140‑1 (MQ=255)                                                                                                                             ttCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTggcg        <  2:163979/140‑1 (MQ=255)                                                                                                                             ttCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTggcg        >  2:853606/1‑140 (MQ=255)                                                                                                                             ttCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTggcg        >  1:163979/1‑140 (MQ=255)                                                                                                                             ttCCTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTggcg        >  2:883877/1‑140 (MQ=255)                                                                                                                               ccTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTggcggc      <  2:100182/140‑1 (MQ=255)                                                                                                                               ccTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTggcggc      <  1:200882/140‑1 (MQ=255)                                                                                                                               ccTCTCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTggcggc      <  1:1088800/140‑1 (MQ=255)                                                                                                                                ctctCCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGgcggcg     <  2:684387/140‑1 (MQ=255)                                                                                                                                  ctcCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGAt   >  2:219572/1‑140 (MQ=255)                                                                                                                                   tcCCCGGTCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGAtt  >  2:11148/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATT  >  NC_000913/4637248‑4637517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑