Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	514,464	A→G	G132G (GGT→GGC)	ybbJ ←	NfeD‑like family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	514,464	0	A	G	100.0%	136.7 / NA	39	G132G (GGT→GGC)	ybbJ	NfeD‑like family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/21);  total (18/21)

CGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAA  >  NC_000913/514334‑514595                                                                                                                                   |                                                                                                                                    cGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCg                                                                                                                            <  2:382978/140‑1 (MQ=255)          cgcCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCg                                                                                                                   <  1:659650/140‑1 (MQ=255)                cTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGgcg                                                                                                             <  2:883528/140‑1 (MQ=255)                    ccGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGa                                                                                                         <  1:1070150/140‑1 (MQ=255)                     cGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGAc                                                                                                        <  1:828946/140‑1 (MQ=255)                     cGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGAc                                                                                                        <  2:880903/140‑1 (MQ=255)                          aTCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGcc                                                                                                   <  2:1147380/140‑1 (MQ=255)                             gAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATg                                                                                                <  2:872814/140‑1 (MQ=255)                              aaaaTCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGa                                                                                               <  2:1035096/140‑1 (MQ=255)                                  tctctcCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTg                                                                                           >  2:616830/1‑140 (MQ=255)                                   ctctcCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGt                                                                                          <  2:880113/140‑1 (MQ=255)                                   ctctcCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGt                                                                                          <  1:306370/140‑1 (MQ=255)                                             gctgTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGAcgcg                                                                                >  1:580593/1‑140 (MQ=255)                                                           gCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGcc                                                                  >  1:130564/1‑140 (MQ=255)                                                           gCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGcc                                                                  >  1:521554/1‑140 (MQ=255)                                                               ggTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGtt                                                              >  2:555332/1‑140 (MQ=255)                                                                     tAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGa                                                            >  1:75308/1‑136 (MQ=255)                                                                     tAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGa                                                            <  2:75308/136‑1 (MQ=255)                                                                             gacgGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGAtt                                                >  2:50819/1‑140 (MQ=255)                                                                                  cTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGc                                           >  1:62534/1‑140 (MQ=255)                                                                                  cTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGc                                           >  2:134630/1‑140 (MQ=255)                                                                                         atGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAAc                                    <  2:1147008/140‑1 (MQ=255)                                                                                            tAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTc                                 >  2:1119448/1‑140 (MQ=255)                                                                                                 ttATCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCa                            >  2:948878/1‑140 (MQ=255)                                                                                                   aTCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGc                              <  2:1146302/136‑1 (MQ=255)                                                                                                   aTCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGc                              >  1:1146302/1‑136 (MQ=255)                                                                                                   aTCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAAt                          <  1:616830/140‑1 (MQ=255)                                                                                                   aTCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAAt                          >  2:812998/1‑140 (MQ=255)                                                                                                   aTCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAACACAAAACGTCGGCCAAt                          >  1:1174701/1‑140 (MQ=255)                                                                                                    tCCCTTCTATCGCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATc                         >  2:729733/1‑140 (MQ=255)                                                                                                               gCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGc                              >  2:60327/1‑124 (MQ=255)                                                                                                               gCAATGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGc                              <  1:60327/124‑1 (MQ=255)                                                                                                                 aaTGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCcgc            <  1:948878/140‑1 (MQ=255)                                                                                                                  aTGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGcc               >  1:678330/1‑136 (MQ=255)                                                                                                                  aTGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGcc               <  2:678330/136‑1 (MQ=255)                                                                                                                  aTGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCcgcg           <  1:1103726/140‑1 (MQ=255)                                                                                                                   tGACTTCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCgcgc          <  1:1119448/140‑1 (MQ=255)                                                                                                                       ttCAACATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGt      >  2:1124694/1‑140 (MQ=255)                                                                                                                           aCATGCGTGCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTaa  <  1:812998/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    CGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAA  >  NC_000913/514334‑514595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑