Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	531,377	A→G	V282A (GTA→GCA)	allS ←	DNA‑binding transcriptional activator AllS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	531,377	0	A	G	100.0%	99.4 / NA	29	V282A (GTA→GCA)	allS	DNA‑binding transcriptional activator AllS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/16);  total (13/16)

CAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTACCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGACCAGTTGTTGATTATCGATCATTGACTGGCAAAGCGATTTTGGCAA  >  NC_000913/531243‑531501                                                                                                                                       |                                                                                                                             cAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCt                                                                                                                         <  2:538457/140‑1 (MQ=255)       aGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCg                                                                                                                   <  1:738037/140‑1 (MQ=255)        gCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGc                                                                                                                  <  2:681823/140‑1 (MQ=255)                 cTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAAtt                                                                                                         <  1:544249/140‑1 (MQ=255)                     tttACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTgcg                                                                                                     <  1:622410/140‑1 (MQ=255)                            gcCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGcc                                                                                              >  2:1104177/1‑140 (MQ=255)                              cAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAg                                                                                            >  2:337747/1‑140 (MQ=255)                               aaCGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGa                                                                                           >  2:567813/1‑140 (MQ=255)                                    gcAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCaa                                                                                      <  1:223407/140‑1 (MQ=255)                                                gATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAaggagg                                                                          >  1:176587/1‑140 (MQ=255)                                                   ttttAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGgcg                                                                       <  2:950346/140‑1 (MQ=255)                                                   ttttAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGgcg                                                                       >  1:1106994/1‑140 (MQ=255)                                                   ttttAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGgcg                                                                       <  1:337747/140‑1 (MQ=255)                                                   ttttAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGgcg                                                                       <  1:567813/140‑1 (MQ=255)                                                                     ccGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCg                                                     >  2:761847/1‑140 (MQ=255)                                                                      cGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCgg                                                    >  2:1037662/1‑140 (MQ=255)                                                                         aaaTTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTg                                                 >  2:28399/1‑140 (MQ=255)                                                                           aTTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGAc                                               <  2:882744/140‑1 (MQ=255)                                                                               cTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGACCAgt                                           >  1:1025376/1‑140 (MQ=255)                                                                                 aaaaaTCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGACCAgttg                                         <  1:499502/140‑1 (MQ=255)                                                                                      tCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGAc                                               <  1:695407/129‑1 (MQ=255)                                                                                      tCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGAc                                               >  2:695407/1‑129 (MQ=255)                                                                                         gCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGACCAGTTGTTGATTAt                                 <  2:595057/140‑1 (MQ=255)                                                                                         gCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGACCAGTTGTTGATTAt                                 <  2:81498/140‑1 (MQ=255)                                                                                          cTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGACCAGTTGTTGATTATc                                <  1:348794/140‑1 (MQ=255)                                                                                               ttCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGACCAGTTGTTGATTATCGATCa                           <  1:1037662/140‑1 (MQ=255)                                                                                                                   aCAAGGTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGACCAGTTGTTGATTATCGATCATTGACTGGCAAAGCGAtttt       >  2:590343/1‑140 (MQ=255)                                                                                                                        gTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGACCAGTTGTTGATTATCGATCATTGACTGGCAAAGCGATTTTGGCaa  >  2:43299/1‑140 (MQ=255)                                                                                                                        gTCACAATATCTTCTGCCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGACCAGTTGTTGATTATCGATCATTGACTGGCAAAGCGATTTTGGCaa  >  1:19422/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                             CAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCCGCTGATTTCCGGCCTGCGCTGGGTAAACAAGGTCACAATATCTTCTACCGCTTTGCCGCTGCCAAATTTGCGCCATGCCAGACTCAATGGCGAAGGAGGGCGCATCGTTGGGATTACCCGGCTGACCAGTTGTTGATTATCGATCATTGACTGGCAAAGCGATTTTGGCAA  >  NC_000913/531243‑531501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑