Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	548,466	A→G	N37S (AAC→AGC)	ylbE →	DUF1116 domain‑containing protein YlbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	548,466	0	A	G	100.0%	195.9 / NA	55	N37S (AAC→AGC)	ylbE	DUF1116 domain‑containing protein YlbE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/25);  total (30/25)

TTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTG  >  NC_000913/548330‑548602                                                                                                                                         |                                                                                                                                         ttaGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGa                                                                                                                                       <  2:832448/140‑1 (MQ=255)       cGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCaa                                                                                                                                 >  2:933544/1‑140 (MQ=255)           tGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGACAAAaca                                                                                                                             >  1:718247/1‑140 (MQ=255)                tAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCt                                                                                                                        <  1:495274/140‑1 (MQ=255)                    gggTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGTGAACTAAGCGAGGGCAAAACACTGCTTCAc                                                                                                                    >  2:848587/1‑140 (MQ=255)                    gggTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCAc                                                                                                                    >  2:388428/1‑140 (MQ=255)                        tCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCg                                                                                                                >  1:143491/1‑140 (MQ=255)                                 tACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa                                                                                                       >  2:481703/1‑140 (MQ=255)                                    aTCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgc                                                                                                    >  2:513250/1‑140 (MQ=255)                                     tCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcg                                                                                                   <  2:143491/140‑1 (MQ=255)                                     tCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcg                                                                                                   <  2:44764/140‑1 (MQ=255)                                         tGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg                                                                                               >  2:587796/1‑140 (MQ=255)                                         tGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg                                                                                               >  1:491176/1‑140 (MQ=255)                                         tGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCCTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg                                                                                               >  1:54613/1‑140 (MQ=255)                                          ggCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGc                                                                                              <  1:587796/140‑1 (MQ=255)                                               aaGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgag                                                                                         <  1:63709/140‑1 (MQ=255)                                                      tgctgcGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg                                                                                  <  1:848587/140‑1 (MQ=255)                                                        ctgcGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGa                                                                                <  1:388428/140‑1 (MQ=255)                                                            ggTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCa                                                                            <  1:481703/140‑1 (MQ=255)                                                             gTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCAt                                                                           >  1:664232/1‑140 (MQ=255)                                                                     aCAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgggg                                                                   >  2:80910/1‑140 (MQ=255)                                                                        aaTTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgt                                                                >  1:604415/1‑140 (MQ=255)                                                                           tcgtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgtgcg                                                             >  2:488538/1‑140 (MQ=255)                                                                               cgcgCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTggg                                                         >  2:420022/1‑140 (MQ=255)                                                                                   cTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCa                                                     >  1:1000245/1‑140 (MQ=255)                                                                                    tCGTGCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCAt                                                    >  2:117025/1‑140 (MQ=255)                                                                                      gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAGCTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGt                                                  <  2:150165/140‑1 (MQ=255)                                                                                      gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGt                                                  <  2:1000245/140‑1 (MQ=255)                                                                                            acTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTc                                            >  1:272777/1‑140 (MQ=255)                                                                                             ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCg                                           <  1:190124/140‑1 (MQ=255)                                                                                              tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGa                                          >  1:771890/1‑140 (MQ=255)                                                                                                gctggATGTGCAACCGGCTTCTTCACTTATCAGCTAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTc                                                 <  2:1116323/131‑1 (MQ=255)                                                                                                gctggATGTGCAACCGGCTTCTTCACTTATCAGCTAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTc                                                 >  1:1116323/1‑131 (MQ=255)                                                                                                gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAg                                        >  2:58093/1‑140 (MQ=255)                                                                                                gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAg                                        >  2:63183/1‑140 (MQ=255)                                                                                                  tggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGt                                      <  1:117025/140‑1 (MQ=255)                                                                                                       gtgCAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGc                                 <  2:304893/140‑1 (MQ=255)                                                                                                          cAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGaa                              >  1:243446/1‑140 (MQ=255)                                                                                                          cAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGaa                              >  1:335360/1‑140 (MQ=255)                                                                                                          cAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGaa                              >  2:120933/1‑140 (MQ=255)                                                                                                          cAACCGGCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGaa                              >  1:1109212/1‑140 (MQ=255)                                                                                                          cAACCGGCTTCTTCACTCATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGaa                              >  1:737750/1‑140 (MQ=255)                                                                                                               ggCTTCTTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATg                         <  2:491176/140‑1 (MQ=255)                                                                                                               ggCTTCCTCACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATg                         <  2:54613/140‑1 (MQ=255)                                                                                                                    cttcACTTATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAgcg                    <  2:771890/140‑1 (MQ=255)                                                                                                                           tATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGgcgc             <  1:80910/140‑1 (MQ=255)                                                                                                                           tATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGgc               <  2:466296/138‑1 (MQ=255)                                                                                                                           tATCAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGgc               >  1:466296/1‑138 (MQ=255)                                                                                                                              cAGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTgg          <  1:488538/140‑1 (MQ=255)                                                                                                                               aGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGgcg              <  2:755517/135‑1 (MQ=255)                                                                                                                               aGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGgcg              >  1:755517/1‑135 (MQ=255)                                                                                                                               aGCGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGc         <  2:654241/140‑1 (MQ=255)                                                                                                                                 cGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGgcg              >  1:261278/1‑133 (MQ=255)                                                                                                                                 cGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGgcg              <  2:261278/133‑1 (MQ=255)                                                                                                                                 cGAACTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCaa       >  1:124672/1‑140 (MQ=255)                                                                                                                                   aaCTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATa     >  1:907546/1‑140 (MQ=255)                                                                                                                                    aCTAAGCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATAc    <  2:272777/140‑1 (MQ=255)                                                                                                                                      tAAGCGAGGACAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCGTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTg  <  2:718247/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTG  >  NC_000913/548330‑548602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑