Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	661,741	A→G	S180P (TCA→CCA)	lipB ←	lipoyl(octanoyl) transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	661,741	0	A	G	100.0%	314.5 / NA	87	S180P (TCA→CCA)	lipB	lipoyl(octanoyl) transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (52/35);  total (52/35)

CAGTGTAAATTGGGCCATTGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGATATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTG  >  NC_000913/661607‑661866                                                                                                                                       |                                                                                                                              cAGTGTAAATTGGGCCATTGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTAttt                                                                                                                          <  2:754605/140‑1 (MQ=255)   gtgtAAATTGGGCCATTGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTa                                                                                                                        >  1:514663/1‑140 (MQ=255)   gtgtAAATTGGGCCATTGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTa                                                                                                                        >  2:1180904/1‑140 (MQ=255)    tgtAAATTGGGCCATTGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAg                                                                                                                       >  1:111344/1‑140 (MQ=255)     gtAAATTGGGCCATTGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGc                                                                                                                      <  1:86038/140‑1 (MQ=255)        aaTTGGGCCATTGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCAt                                                                                                                   >  2:698541/1‑140 (MQ=255)        aaTTGGGCCATTGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCAt                                                                                                                   <  2:616583/140‑1 (MQ=255)         aTTGGGCCATTGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCAtt                                                                                                                  >  1:805736/1‑140 (MQ=255)              gCCATTGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCAt                                                                                                                   <  1:731371/134‑1 (MQ=255)              gCCATTGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCAt                                                                                                                   >  2:731371/1‑134 (MQ=255)                  ttGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCg                                                                                                         >  1:1096528/1‑140 (MQ=255)                  ttGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCg                                                                                                         >  2:490465/1‑140 (MQ=255)                        tATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATAc                                                                                                   <  2:215249/140‑1 (MQ=255)                        tATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATAc                                                                                                   >  1:372294/1‑140 (MQ=255)                          tGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATAccc                                                                                                 >  2:900544/1‑140 (MQ=255)                            gAATTAAGTGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCcac                                                                                               <  2:337427/140‑1 (MQ=255)                            gAATTAAGCGGTAATATATTCGAAGTCCAGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATAc                                                                                                   >  2:61569/1‑136 (MQ=255)                            gAATTAAGCGGTAATATATTCGAAGTCCAGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATAc                                                                                                   <  1:61569/136‑1 (MQ=255)                             aaTTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCcaca                                                                                              <  2:1050144/140‑1 (MQ=255)                             aaTTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCcaca                                                                                              <  2:514663/140‑1 (MQ=255)                             aaTTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCcaca                                                                                              >  1:1050144/1‑140 (MQ=255)                               ttAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATa                                                                                                    <  2:17106/132‑1 (MQ=255)                               ttAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATa                                                                                                    >  1:17106/1‑132 (MQ=255)                               ttAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAg                                                                                            >  2:557364/1‑140 (MQ=255)                                 aaGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGa                                                                                          >  2:916719/1‑140 (MQ=255)                                   gCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGAtt                                                                                        >  2:924702/1‑140 (MQ=255)                                    cGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCca                                                                                                >  2:839940/1‑131 (MQ=255)                                    cGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCca                                                                                                <  1:839940/131‑1 (MQ=255)                                    cGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTa                                                                                       >  2:1016399/1‑140 (MQ=255)                                    cGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTa                                                                                       <  2:1096528/140‑1 (MQ=255)                                    cGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTa                                                                                       <  1:1097036/140‑1 (MQ=255)                                       tAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATa                                                                                    >  1:561229/1‑140 (MQ=255)                                         atatatTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACg                                                                                  >  2:1014481/1‑140 (MQ=255)                                                 gAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTaaaa                                                                             <  2:171969/137‑1 (MQ=255)                                                 gAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTaaaa                                                                             >  1:171969/1‑137 (MQ=255)                                                 gAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATg                                                                          >  1:790101/1‑140 (MQ=255)                                                   aGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGt                                                                        >  2:482932/1‑140 (MQ=255)                                                   aGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGt                                                                        <  2:1185860/140‑1 (MQ=255)                                                     tCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGa                                                                      >  2:466546/1‑140 (MQ=255)                                                     tCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGa                                                                      <  1:1014481/140‑1 (MQ=255)                                                      ccGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGaa                                                                     <  1:1180904/140‑1 (MQ=255)                                                          aTTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGAt                                                                 <  2:790101/140‑1 (MQ=255)                                                          aTTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGAt                                                                 >  1:595474/1‑140 (MQ=255)                                                           ttGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATc                                                                <  2:372294/140‑1 (MQ=255)                                                              tttAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCat                                                             >  1:305729/1‑140 (MQ=255)                                                                   tagCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTA‑TTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGAc                                                       >  2:542621/1‑140 (MQ=255)                                                                     gcgcTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACg                                                      >  2:321527/1‑140 (MQ=255)                                                                      cgcTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGt                                                     >  2:175409/1‑140 (MQ=255)                                                                       gcTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGtt                                                    <  1:924702/140‑1 (MQ=255)                                                                       gcTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGtt                                                    <  1:175409/140‑1 (MQ=255)                                                                       gcTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGtt                                                    <  2:766236/140‑1 (MQ=255)                                                                        cTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGttt                                                   >  2:340234/1‑140 (MQ=255)                                                                        cTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGttt                                                   >  2:931655/1‑140 (MQ=255)                                                                             atatTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTA‑TTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGcc                                             <  1:542621/140‑1 (MQ=255)                                                                               atTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCa                                            <  1:321527/140‑1 (MQ=255)                                                                                   tCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGAcc                                        >  2:671848/1‑140 (MQ=255)                                                                                   tCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGAcc                                        >  1:1075858/1‑140 (MQ=255)                                                                                   tCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTACATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGAcc                                        >  1:485580/1‑140 (MQ=255)                                                                                      aGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTg                                     <  2:1047327/140‑1 (MQ=255)                                                                                      aGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTg                                     <  2:908187/140‑1 (MQ=255)                                                                                        tAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGa                                          >  2:706309/1‑133 (MQ=255)                                                                                        tAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGa                                          <  1:706309/133‑1 (MQ=255)                                                                                        tAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGa                                   >  1:303634/1‑140 (MQ=255)                                                                                               ggAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGaaca                            >  1:1149036/1‑140 (MQ=255)                                                                                                  gCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAAcc                         >  1:879393/1‑140 (MQ=255)                                                                                                          attaGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGaa                 <  1:490465/140‑1 (MQ=255)                                                                                                          attaGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGaa                 <  2:561229/140‑1 (MQ=255)                                                                                                           ttaGTCGTCGCTTCGGGTTTCCATTGTGGAATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAAt                >  1:601675/1‑140 (MQ=255)                                                                                                             aGTCGTCGCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATAc              >  1:620503/1‑140 (MQ=255)                                                                                                                cgtcgCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGCATGAACAACCGCGTCGAATACGTa           >  1:1050870/1‑140 (MQ=255)                                                                                                                cgtcgCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTa           >  2:387198/1‑140 (MQ=255)                                                                                                                cgtcgCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTa           >  1:680771/1‑140 (MQ=255)                                                                                                                cgtcgCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTa           >  2:909385/1‑140 (MQ=255)                                                                                                                cgtcgCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTa           >  1:809824/1‑140 (MQ=255)                                                                                                                cgtcgCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTa           >  2:607033/1‑140 (MQ=255)                                                                                                                cgtcgCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTa           >  1:306416/1‑140 (MQ=255)                                                                                                                cgtcgCTTCCGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACg             >  1:915909/1‑138 (MQ=255)                                                                                                                cgtcgCTTCCGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACg             <  2:915909/138‑1 (MQ=255)                                                                                                                  tcgCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGaa                 >  1:895192/1‑132 (MQ=255)                                                                                                                  tcgCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGaa                 >  1:1099389/1‑132 (MQ=255)                                                                                                                  tcgCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGaa                 <  2:895192/132‑1 (MQ=255)                                                                                                                  tcgCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGaa                 <  2:1099389/132‑1 (MQ=255)                                                                                                                  tcgCTTCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTaaa         >  1:51893/1‑140 (MQ=255)                                                                                                                      ttCGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCa     <  1:607033/140‑1 (MQ=255)                                                                                                                        cGGGTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGt   >  2:732992/1‑140 (MQ=255)                                                                                                                         gggTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTg  <  1:671848/140‑1 (MQ=255)                                                                                                                         gggTTTCCATTGTGGTATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTg  <  2:595474/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                              CAGTGTAAATTGGGCCATTGATGTATGGAATTAAGCGGTAATATATTCGAAGTCCGGATTGTTTAGTAGCGCTAAAATATTTTCCAGTAAACGTGGAGCAATATTATTAGTCGTCGCTTCGGGTTTCCATTGTGATATTTTAGCCATTTCCATTCCGGCATACCCACAAGGATTAATACGTAAAAATGGTGAAAGATCCATATTGACGTTTAATGCCAGACCGTGGAATGAACAACCGCGTCGAATACGTAAACCCAGTG  >  NC_000913/661607‑661866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑