Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	684,717	A→G	V180A (GTG→GCG)	gltL ←	glutamate/aspartate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	684,717	0	A	G	100.0%	206.4 / NA	58	V180A (GTG→GCG)	gltL	glutamate/aspartate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/34);  total (24/34)

CATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAG  >  NC_000913/684580‑684847                                                                                                                                           |                                                                                                                                   catcGAAGAAAGCGTCTTTCGGCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTccgcc                                                                                                                                  >  1:527613/1‑140 (MQ=255)    cGAAGAAAGCGTCTTTCGGCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATc                                                                                                                               <  1:1105959/140‑1 (MQ=255)       agaaAGCGTCTTTCGGCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCA‑CATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGt                                                                                                                           >  2:5985/1‑140 (MQ=255)         aaaGCGTCTTTCGGCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTc                                                                                                                          <  2:905360/140‑1 (MQ=255)         aaaGCGTCTTTCGGCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTc                                                                                                                          <  1:273891/140‑1 (MQ=255)          aaGCGTCTTTCGGCGAGTCTTCGACAAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTc                                                                                                                          <  2:701677/140‑1 (MQ=255)             cGTCTTTCGGCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGt                                                                                                                      >  1:836994/1‑140 (MQ=255)               tCTTTCGGCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTAc                                                                                                                    <  1:198894/140‑1 (MQ=255)                 tttCGGCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACtt                                                                                                                  <  1:744710/140‑1 (MQ=255)                   tCGGCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCTACTTTCCGGGCAAACCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCGCGTCCAGTACTTCg                                                                                                                <  2:568742/140‑1 (MQ=255)                    cGGCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCA‑CATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGtt                                                                                                              <  1:5985/140‑1 (MQ=255)                     ggCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCg                                                                                                                <  1:942309/138‑1 (MQ=255)                     ggCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCg                                                                                                                >  2:942309/1‑138 (MQ=255)                         aGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGatc                                                                                                          <  1:287418/140‑1 (MQ=255)                                        ttACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAg                                                                                            >  2:143705/1‑140 (MQ=255)                                         tACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAgc                                                                                           >  2:398444/1‑140 (MQ=255)                                           cccTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAgcgc                                                                                         <  1:143705/140‑1 (MQ=255)                                                gTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATg                                                                                    >  1:176079/1‑140 (MQ=255)                                                 tCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGt                                                                                   <  2:176079/140‑1 (MQ=255)                                                     tAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGt                                                                               <  2:395753/140‑1 (MQ=255)                                                        aGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCg                                                                            <  1:188129/140‑1 (MQ=255)                                                          aTCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTc                                                                          >  2:956682/1‑140 (MQ=255)                                                            cACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaa                                                                        >  2:107709/1‑140 (MQ=255)                                                            cACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaa                                                                        >  1:144761/1‑140 (MQ=255)                                                            cACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaa                                                                        >  2:956416/1‑140 (MQ=255)                                                             aCCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaaa                                                                       <  1:926834/140‑1 (MQ=255)                                                             aCCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaaa                                                                       >  2:926834/1‑140 (MQ=255)                                                                cGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAg                                                                    <  1:107709/140‑1 (MQ=255)                                                                  aTTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCa                                                                  <  2:527613/140‑1 (MQ=255)                                                                   ttCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCAt                                                                 <  1:428641/140‑1 (MQ=255)                                                                    tCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCAt                                                                 >  1:828850/1‑139 (MQ=255)                                                                    tCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCAt                                                                 <  2:828850/139‑1 (MQ=255)                                                                      gCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGc                                                              >  1:987441/1‑140 (MQ=255)                                                                         aCTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAAt                                                           <  2:987441/140‑1 (MQ=255)                                                                         aCTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAAt                                                           <  2:1109931/140‑1 (MQ=255)                                                                           tttACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                         <  1:263315/140‑1 (MQ=255)                                                                           tttACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                         <  1:346527/140‑1 (MQ=255)                                                                           tttACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                         <  2:563530/140‑1 (MQ=255)                                                                           tttACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                         <  2:433733/140‑1 (MQ=255)                                                                           tttACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                         <  1:956416/140‑1 (MQ=255)                                                                           tttACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                         <  2:144761/140‑1 (MQ=255)                                                                             tACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGa                                                       <  2:236013/140‑1 (MQ=255)                                                                             tACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGa                                                       >  1:1144621/1‑140 (MQ=255)                                                                                 ggCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCa                                                   >  1:573775/1‑140 (MQ=255)                                                                                  gCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCAt                                                  >  2:496292/1‑140 (MQ=255)                                                                                     aaGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATc                                                                >  2:954676/1‑123 (MQ=255)                                                                                     aaGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATc                                                                <  1:954676/123‑1 (MQ=255)                                                                                     aaGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATaca                                               >  2:764779/1‑140 (MQ=255)                                                                                                   ggTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCgcgagcga                                 <  1:414500/140‑1 (MQ=255)                                                                                                         caccaTCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCca                           <  1:764779/140‑1 (MQ=255)                                                                                                         caccaTCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCca                           <  1:496292/140‑1 (MQ=255)                                                                                                           ccaTCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCcaca                         <  1:251418/140‑1 (MQ=255)                                                                                                           ccaTCATGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCcaca                         <  1:605122/140‑1 (MQ=255)                                                                                                             atcatGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACg                       <  2:1144621/140‑1 (MQ=255)                                                                                                              tcatGGTCATTCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACgc                      >  2:1067021/1‑140 (MQ=255)                                                                                                                        tCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAc            >  2:361293/1‑140 (MQ=255)                                                                                                                        tCCTTCGTTCGCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAc            >  1:1123068/1‑140 (MQ=255)                                                                                                                            tcgttcgCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAccgcc        >  1:1005238/1‑140 (MQ=255)                                                                                                                                  gCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAg  >  1:855750/1‑140 (MQ=255)                                                                                                                                  gCCAGTTCCGCCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAg  >  2:945871/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                   CATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGAC‑AATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAG  >  NC_000913/684580‑684847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑