Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	724,939	A→G	L14P (CTG→CCG)	kdpC ←	K(+) transporting P‑type ATPase subunit KdpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	724,939	0	A	G	100.0%	170.3 / NA	48	L14P (CTG→CCG)	kdpC	K(+) transporting P‑type ATPase subunit KdpC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/26);  total (22/26)

TAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACCAGCAGCCCACCCAGACCGTAAATCC  >  NC_000913/724802‑725073                                                                                                                                          |                                                                                                                                       tAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGa                                                                                                                                      <  1:706331/140‑1 (MQ=255)      ttCTGCCCGATTAATGCCGAACCACGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGAt                                                                                                                                 >  1:210486/1‑140 (MQ=255)              gATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTg                                                                                                                         >  2:1156348/1‑140 (MQ=255)                ttAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGAt                                                                                                                       <  1:18479/140‑1 (MQ=255)                     gCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGc                                                                                                                  <  2:663286/140‑1 (MQ=255)                          aCCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGAc                                                                                                             <  2:1089354/140‑1 (MQ=255)                          aCCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGAc                                                                                                             <  1:447720/140‑1 (MQ=255)                               gcACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAAt                                                                                                        <  2:459217/140‑1 (MQ=255)                               gcACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAAt                                                                                                        <  2:168566/140‑1 (MQ=255)                                   cGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCAc                                                                                                    <  2:210486/140‑1 (MQ=255)                                     tATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACg                                                                                                            >  1:177441/1‑130 (MQ=255)                                     tATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACg                                                                                                            <  2:177441/130‑1 (MQ=255)                                          ccTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGt                                                                                             <  1:912069/140‑1 (MQ=255)                                          ccTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGt                                                                                             <  1:651670/140‑1 (MQ=255)                                           cTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTa                                                                                            <  2:90857/140‑1 (MQ=255)                                           cTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTa                                                                                            <  2:911792/140‑1 (MQ=255)                                                                tGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCaaa                                                                       <  1:469443/140‑1 (MQ=255)                                                                 ggCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAAc                                                                      >  2:31588/1‑140 (MQ=255)                                                                      gCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGt                                                                   >  2:727852/1‑138 (MQ=255)                                                                      gCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGt                                                                   <  1:727852/138‑1 (MQ=255)                                                                       ccAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATAGTAAACCTCACACCAGACCGCAAACGGTcag                                                                >  1:332014/1‑140 (MQ=255)                                                                            gAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTa                                                           >  2:192893/1‑140 (MQ=255)                                                                            gAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTa                                                           >  2:1027205/1‑140 (MQ=255)                                                                                   ccaTTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAAt                                                    <  1:710265/140‑1 (MQ=255)                                                                                   ccaTTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGACAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAAt                                                    <  1:142302/140‑1 (MQ=255)                                                                                     aTTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTc                                                                  >  1:987314/1‑124 (MQ=255)                                                                                     aTTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTc                                                                  <  2:987314/124‑1 (MQ=255)                                                                                         ccccAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACttt                                              >  1:567086/1‑140 (MQ=255)                                                                                           ccAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGa                                            <  1:766714/140‑1 (MQ=255)                                                                                              gTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCa                                                      >  1:340178/1‑127 (MQ=255)                                                                                              gTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCa                                                      <  2:340178/127‑1 (MQ=255)                                                                                                   gtggtCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATa                                    >  2:331285/1‑140 (MQ=255)                                                                                                       tCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACg                                >  2:1935/1‑140 (MQ=255)                                                                                                         agcagcGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGc                              >  2:797590/1‑140 (MQ=255)                                                                                                         agcagcGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGc                              >  2:736474/1‑140 (MQ=255)                                                                                                         agcagcGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGc                              >  1:768661/1‑140 (MQ=255)                                                                                                         agcagcGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACAACAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGc                              >  2:887639/1‑140 (MQ=255)                                                                                                             gcGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACca                          <  1:31588/140‑1 (MQ=255)                                                                                                              cGGGTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACcag                         >  1:615544/1‑140 (MQ=255)                                                                                                                ggTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACcagca                       >  1:882119/1‑140 (MQ=255)                                                                                                                ggTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACcagca                       >  1:910943/1‑140 (MQ=255)                                                                                                                ggTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACcagca                       >  1:1187073/1‑140 (MQ=255)                                                                                                                 gTAAACGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACcagcag                      <  1:736474/140‑1 (MQ=255)                                                                                                                     aCGCCGCCAGTAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACCAGCAGccca                  >  1:244914/1‑140 (MQ=255)                                                                                                                               tAATCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACCAGCAGCCCACCCAGACCGt        <  1:1027205/140‑1 (MQ=255)                                                                                                                                aaTCAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACCAGCAGCCCACCCAGACCGTa       <  2:615544/140‑1 (MQ=255)                                                                                                                                   caacaaCAGAAATATAAATCCACATAACTCCCCACTTAATCCACTCACCCTAAACCTCACACCAGACCGCAAACACTCACCAACAAACCAATTACCTTCACACCGATAAACCTCCCCACCACCCCACCCAGCCCCTaaac    <  2:1048620/140‑2 (MQ=255)                                                                                                                                   cAATAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACCAGCAGCCCACCCAGACCGTAAAt    <  2:720327/140‑1 (MQ=255)                                                                                                                                     aTAACGGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACCAGCAGCCCACCCAGACCGTAAATcc  <  2:768661/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TAAAATTCTGCCCGATTAATGCCGAACCGCGCACCGTATCACCTTCACGAATCAACGAACCATTGGCCTGCCAGGGAAACCACCATTGCCCCAGTACGGTGGTCAGCAGCGGGTAAACGCCGCCAGTAATCAATAACAGAAAGATAAATGTTGATAATGCCGGACGTAATCCACTCATGGTAAACCTCACACCAGACCGCAAACGGTCAGCAGTAAATCAATGACTTTGATACCGATAAACGGCACCAGCAGCCCACCCAGACCGTAAATCC  >  NC_000913/724802‑725073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑