Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 744,943 T→C V234A (GTG→GCG)  pxpC → 5‑oxoprolinase component C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913744,9430TC100.0% 197.2 / NA 56V234A (GTG→GCG) pxpC5‑oxoprolinase component C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/30);  total (26/30)

CTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTC  >  NC_000913/744806‑745077
                                                                                                                                         |                                                                                                                                      
cTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGgcgc                                                                                                                                      <  1:520834/140‑1 (MQ=255)
    cAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGt                                                                                                                                  >  1:624088/1‑140 (MQ=255)
      ggATGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGc                                                                                                                                <  2:727472/140‑1 (MQ=255)
      ggATGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGc                                                                                                                                <  1:1083503/140‑1 (MQ=255)
       gATGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGcc                                                                                                                               >  1:1131267/1‑140 (MQ=255)
        aTGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCca                                                                                                                              >  2:1172373/1‑140 (MQ=255)
          gCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCcaca                                                                                                                            >  2:1208425/1‑140 (MQ=255)
                   cGTTCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCa                                                                                                                   >  1:312880/1‑140 (MQ=255)
                   cGTTCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCa                                                                                                                   <  2:1131267/140‑1 (MQ=255)
                     ttCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGc                                                                                                                 >  1:243051/1‑140 (MQ=255)
                     ttCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGc                                                                                                                 >  1:879412/1‑140 (MQ=255)
                     ttCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGGGCCACATAACGGGAAGc                                                                                                                 >  2:353034/1‑140 (MQ=255)
                      tCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGcc                                                                                                                <  2:1110499/140‑1 (MQ=255)
                        gCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGa                                                                                                              >  1:739743/1‑140 (MQ=255)
                          ccTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGAtt                                                                                                            <  1:917467/140‑1 (MQ=255)
                                  cTTGGCTCACAAAGAAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTtgat                                                                                                    >  2:51887/1‑140 (MQ=255)
                                     aGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGaa                                                                                                 <  2:783983/140‑1 (MQ=255)
                                            aaaGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAacg                                                                                               >  2:1197065/1‑135 (MQ=255)
                                            aaaGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAacg                                                                                               <  1:1197065/135‑1 (MQ=255)
                                            aaaGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGca                                                                                          <  2:1025642/140‑1 (MQ=255)
                                                    cGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGaccac                                                                                  <  1:1208425/140‑1 (MQ=255)
                                                    cGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGaccac                                                                                  <  2:1181206/140‑1 (MQ=255)
                                                      cATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCg                                                                                >  2:762609/1‑140 (MQ=255)
                                                              aTCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTAc                                                                        >  1:1186757/1‑140 (MQ=255)
                                                               tCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTAcc                                                                       >  1:760282/1‑140 (MQ=255)
                                                               tCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTAcc                                                                       <  1:1172373/140‑1 (MQ=255)
                                                               tCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTAcc                                                                       <  1:631499/140‑1 (MQ=255)
                                                                 gCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCcg                                                                     <  2:624088/140‑1 (MQ=255)
                                                                     aCAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTa                                                                 <  1:502110/140‑1 (MQ=255)
                                                                     aCAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTa                                                                 >  2:189427/1‑140 (MQ=255)
                                                                     aCAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTa                                                                 <  2:148875/140‑1 (MQ=255)
                                                                     aCAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTa                                                                 <  1:189427/140‑1 (MQ=255)
                                                                       aGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTAtt                                                               <  2:879412/140‑1 (MQ=255)
                                                                         gggCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGc                                                             <  2:243051/140‑1 (MQ=255)
                                                                         gggCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGc                                                             <  2:312880/140‑1 (MQ=255)
                                                                         gggCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGc                                                             <  2:760282/140‑1 (MQ=255)
                                                                         gggCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGAGGCGCAGGTGCCACATAGCGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGc                                                             >  2:1087453/1‑140 (MQ=255)
                                                                           gCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTAcc                                                                       <  2:785404/128‑1 (MQ=255)
                                                                           gCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTAcc                                                                       >  1:785404/1‑128 (MQ=255)
                                                                                       aCGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAgg                                               <  2:114310/140‑1 (MQ=255)
                                                                                       aCGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAgg                                               <  1:51887/140‑1 (MQ=255)
                                                                                          caccacCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTg                                            <  1:849491/140‑1 (MQ=255)
                                                                                            ccaccGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGat                                          <  2:492503/140‑1 (MQ=255)
                                                                                                             ttATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCa                         >  1:642896/1‑140 (MQ=255)
                                                                                                             ttATCTCACGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCa                         >  2:520893/1‑140 (MQ=255)
                                                                                                                    aCGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCg                  <  2:1186757/140‑1 (MQ=255)
                                                                                                                     cGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGc                 >  1:732289/1‑140 (MQ=255)
                                                                                                                     cGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGc                 >  1:446007/1‑140 (MQ=255)
                                                                                                                     cGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGc                 >  2:46959/1‑140 (MQ=255)
                                                                                                                     cGGTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGc                 >  2:220702/1‑140 (MQ=255)
                                                                                                                       gTTTGTTACCGGGCGTGGCGCAGGTGCCATATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTc               >  2:323590/1‑140 (MQ=255)
                                                                                                                       gTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCt                >  2:358725/1‑139 (MQ=255)
                                                                                                                       gTTTGTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCt                <  1:358725/139‑1 (MQ=255)
                                                                                                                           gTTACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTc           <  2:732289/140‑1 (MQ=255)
                                                                                                                             tACCGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAg         <  2:312226/140‑1 (MQ=255)
                                                                                                                                cGGGCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCg      <  2:446007/140‑1 (MQ=255)
                                                                                                                                  ggCGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGAt    >  2:619751/1‑140 (MQ=255)
                                                                                                                                    cGTGGCGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTc  >  2:321951/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
CTCGCAGGATGCATTCTGGCGTTCGCCCTGGCAGCTTAGCTCGCAAAGTAACCGCATGGGCTATCGCTTACAGGGGCAAATTTTAAAACGCACCACCGATCGCGAACTGTTATCTCACGGTTTGTTACCGGGCGTGGTGCAGGTGCCACATAACGGGCAGCCGATTGTGTTGATGAACGACGCACAGACCACCGGTGGTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTC  >  NC_000913/744806‑745077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑