Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	745,130	A→G	Q296Q (CAA→CAG)	pxpC →	5‑oxoprolinase component C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	745,130	0	A	G	100.0%	206.5 / NA	58	Q296Q (CAA→CAG)	pxpC	5‑oxoprolinase component C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/32);  total (26/32)

GTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTT  >  NC_000913/745003‑745260                                                                                                                                |                                                                                                                                   gtTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGaa                                                                                                                        >  2:925152/1‑140 (MQ=255)      ccGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAAtt                                                                                                                   >  2:792523/1‑140 (MQ=255)        gcgTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTg                                                                                                                          >  1:331567/1‑131 (MQ=255)        gcgTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTg                                                                                                                          <  2:331567/131‑1 (MQ=255)        gcgTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAg                                                                                                                 >  2:302051/1‑140 (MQ=255)           tATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGt                                                                                                              >  2:1149805/1‑140 (MQ=255)              tGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTggc                                                                                                           >  1:373312/1‑140 (MQ=255)                ccTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTggcgg                                                                                                         <  1:490923/140‑1 (MQ=255)                    tATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGc                                                                                                     <  1:537561/140‑1 (MQ=255)                    tATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGc                                                                                                     >  2:522528/1‑140 (MQ=255)                    tATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGc                                                                                                     <  2:642896/140‑1 (MQ=255)                     aTCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGca                                                                                                    <  1:353034/140‑1 (MQ=255)                     aTCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGca                                                                                                    <  2:989938/140‑1 (MQ=255)                          tGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATg                                                                                               <  2:888464/140‑1 (MQ=255)                          tGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATg                                                                                               >  2:684755/1‑140 (MQ=255)                              gCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTggcgg                                                                                                         >  2:432615/1‑126 (MQ=255)                              gCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTggcgg                                                                                                         <  1:432615/126‑1 (MQ=255)                                  atatGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGa                                                                                       >  1:764199/1‑140 (MQ=255)                                      gTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTg                                                                                   <  2:373312/140‑1 (MQ=255)                                        aCCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGaa                                                                                 >  1:1020021/1‑140 (MQ=255)                                        aCCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGaa                                                                                 >  1:352735/1‑140 (MQ=255)                                            tCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGTGGCTGCACAATGAAAATTGACCTGAACGcc                                                                             >  1:540628/1‑140 (MQ=255)                                            tCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGcc                                                                             >  1:581346/1‑140 (MQ=255)                                            tCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGcc                                                                             >  1:939646/1‑140 (MQ=255)                                            tCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGcc                                                                             >  2:1180073/1‑140 (MQ=255)                                             cTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCg                                                                            >  1:688608/1‑140 (MQ=255)                                               ggCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGAt                                                                          >  2:533091/1‑140 (MQ=255)                                                gcgcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGTGGCTGCACAATGAAAATTGACCTGAACGCCGATc                                                                         <  2:540628/140‑1 (MQ=255)                                                gcgcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATc                                                                         <  1:522528/140‑1 (MQ=255)                                                gcgcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATc                                                                         <  2:688608/140‑1 (MQ=255)                                                gcgcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATc                                                                         <  1:323590/140‑1 (MQ=255)                                                 cgcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGAc                                                                                      >  1:223995/1‑126 (MQ=255)                                                 cgcAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGAc                                                                                      <  2:223995/126‑1 (MQ=255)                                                                   aGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAg                                                      <  1:1087453/140‑1 (MQ=255)                                                                       gATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGAc                                                  <  2:1020021/140‑1 (MQ=255)                                                                        aTTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACg                                                 <  2:352735/140‑1 (MQ=255)                                                                         ttCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGc                                                <  2:89435/140‑1 (MQ=255)                                                                         ttCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGc                                                <  1:684755/140‑1 (MQ=255)                                                                           cATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCag                                              >  2:708358/1‑140 (MQ=255)                                                                             ttttGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCagag                                            <  1:762609/140‑1 (MQ=255)                                                                                 gTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTAt                                        <  1:302051/140‑1 (MQ=255)                                                                                   ccAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTa                                      >  1:953958/1‑140 (MQ=255)                                                                                      gtgtTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACg                                   <  2:764199/140‑1 (MQ=255)                                                                                              tGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTTAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTc                           <  1:42590/140‑1 (MQ=255)                                                                                               ggAAGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTcc                          <  2:739743/140‑1 (MQ=255)                                                                                                 aaGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCctc                        >  1:724662/1‑140 (MQ=255)                                                                                                 aaGAGGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCctc                        <  1:533091/140‑1 (MQ=255)                                                                                                   gagGCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTg                      >  2:391768/1‑140 (MQ=255)                                                                                                      gCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTaa                                     <  2:410057/122‑1 (MQ=255)                                                                                                      gCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTaa                                     >  1:410057/1‑122 (MQ=255)                                                                                                      gCACTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCa                   >  2:304289/1‑140 (MQ=255)                                                                                                        aCTAAAAGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAat                 >  1:945822/1‑140 (MQ=255)                                                                                                             aaGCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGc            <  2:957748/140‑1 (MQ=255)                                                                                                               gCACGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCt          <  2:939646/140‑1 (MQ=255)                                                                                                                 aCGGCAAGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCtgt        <  1:220702/140‑1 (MQ=255)                                                                                                                      aaGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGAtt   <  1:792523/140‑1 (MQ=255)                                                                                                                       aGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGAttt  <  1:619751/140‑1 (MQ=255)                                                                                                                       aGATCAGCAGCGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGAttt  <  1:1149805/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                   GTTACCCGCGTATTGCCTGTATCATTGAGGCTGATATGTACCATCTGGCGCAAATTCCGCTCGGTCAGCCGATTCATTTTGTCCAGTGTTCACTGGAAGAGGCACTAAAAGCACGGCAAGATCAGCAACGTTATTTTGAACAATTAGCGTGGCGGCTGCACAATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTT  >  NC_000913/745003‑745260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑