Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	800,192	T→C	E128E (GAA→GAG)	ybhD ←	putative DNA‑binding transcriptional regulator YbhD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	800,192	0	T	C	100.0%	217.6 / NA	61	E128E (GAA→GAG)	ybhD	putative DNA‑binding transcriptional regulator YbhD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/35);  total (26/35)

CGCCGACAGGCCAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACGATGATAAGCATTCATAT  >  NC_000913/800058‑800319                                                                                                                                       |                                                                                                                                cGCCGACAGGCCAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAg                                                                                                                            >  1:168950/1‑140 (MQ=255) cGCCGACAGGCCAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAg                                                                                                                            >  2:742718/1‑140 (MQ=255)     gACAGGCCAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATc                                                                                                                        <  2:486747/140‑1 (MQ=255)      aCAGGCCAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATcc                                                                                                                       >  2:773372/1‑140 (MQ=255)       cAGGCCAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCg                                                                                                                      <  2:813617/140‑1 (MQ=255)       cAGGCCAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCg                                                                                                                      <  2:274867/140‑1 (MQ=255)       cAGGCCAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCg                                                                                                                      <  1:132370/140‑1 (MQ=255)       cAGGCCAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCg                                                                                                                      <  1:269127/140‑1 (MQ=255)        aGGCCAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGc                                                                                                                     <  1:1137994/140‑1 (MQ=255)            cAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATc                                                                                                                        >  2:1160436/1‑133 (MQ=255)            cAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATc                                                                                                                        <  1:1160436/133‑1 (MQ=255)            cAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACtt                                                                                                                 <  1:742718/140‑1 (MQ=255)                  gAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAatat                                                                                                           <  1:1089278/140‑1 (MQ=255)                  gAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAatat                                                                                                           <  1:803713/140‑1 (MQ=255)                    aCGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCg                                                                                                                      <  1:939794/127‑1 (MQ=255)                    aCGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCg                                                                                                                      >  2:939794/1‑127 (MQ=255)                    aCGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAata                                                                                                            >  1:776805/1‑137 (MQ=255)                    aCGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAata                                                                                                            <  2:776805/137‑1 (MQ=255)                    aCGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTg                                                                                                         >  2:219170/1‑140 (MQ=255)                            ttGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAg                                                                                                 <  1:403125/140‑1 (MQ=255)                             tGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGc                                                                                                >  1:1046960/1‑140 (MQ=255)                                    tGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTaa                                                                                         >  1:96769/1‑140 (MQ=255)                                       ggTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAAtt                                                                                      >  2:452589/1‑140 (MQ=255)                                                       gAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCgg                                                                      >  1:246265/1‑140 (MQ=255)                                                           ttGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTaaa                                                                  <  1:773372/140‑1 (MQ=255)                                                              tgACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTaaaaaa                                                               >  1:894953/1‑140 (MQ=255)                                                              tgACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTaaaaaa                                                               >  1:522027/1‑140 (MQ=255)                                                               gACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAaaaat                                                              <  2:264887/140‑1 (MQ=255)                                                                          tGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCg                                                   >  2:238965/1‑140 (MQ=255)                                                                                tGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGa                                             >  1:228167/1‑140 (MQ=255)                                                                                tGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGa                                             >  1:784148/1‑140 (MQ=255)                                                                                tGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGa                                             >  1:366504/1‑140 (MQ=255)                                                                                tGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGa                                             >  1:149513/1‑140 (MQ=255)                                                                                    aaaGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATac                                         <  2:1152648/140‑1 (MQ=255)                                                                                       gTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACg                                      <  1:452589/140‑1 (MQ=255)                                                                                         cTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCt                                    <  2:149513/140‑1 (MQ=255)                                                                                           gATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTaa                                  <  1:766627/140‑1 (MQ=255)                                                                                              tCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGt                               <  2:522027/140‑1 (MQ=255)                                                                                                gTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCa                             <  2:1046960/140‑1 (MQ=255)                                                                                                        gCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCa                     <  1:219170/140‑1 (MQ=255)                                                                                                        gCACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCa                     <  1:1206841/140‑1 (MQ=255)                                                                                                         cACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATAcc                      >  1:1039397/1‑138 (MQ=255)                                                                                                         cACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATAcc                      <  2:1039397/138‑1 (MQ=255)                                                                                                         cACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCAc                    >  2:1050597/1‑140 (MQ=255)                                                                                                         cACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCAc                    >  1:209150/1‑140 (MQ=255)                                                                                                         cACCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCAc                    >  1:175855/1‑140 (MQ=255)                                                                                                         cACCGATTCCATGCAAGTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCAc                    <  1:1032626/140‑1 (MQ=255)                                                                                                          aCCGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACg                   <  1:238965/140‑1 (MQ=255)                                                                                                            cGATTCCATGCAATTATTCGTGCCTTGTTCCTGTTTCCGTTCTTTAATCTTGGGATACAGCTCATTAAATTTATCGATTTCCTGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTCACGGCACCATACCACgat                 >  2:264356/1‑140 (MQ=255)                                                                                                            cGATTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACgat                 >  2:688940/1‑140 (MQ=255)                                                                                                              aTTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACgatga               >  2:633549/1‑140 (MQ=255)                                                                                                              aTTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACgat                 >  2:1041212/1‑138 (MQ=255)                                                                                                              aTTCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACgat                 <  1:1041212/138‑1 (MQ=255)                                                                                                               ttCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACgatgat              <  2:246265/140‑1 (MQ=255)                                                                                                               ttCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACgatgat              <  2:366504/140‑1 (MQ=255)                                                                                                               ttCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACgatgat              <  2:497832/140‑1 (MQ=255)                                                                                                               ttCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACgatgat              <  1:323967/140‑1 (MQ=255)                                                                                                                tCCATGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACGATGATa             <  2:96769/140‑1 (MQ=255)                                                                                                                    tGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACGATGATAAGCa         <  2:584758/140‑1 (MQ=255)                                                                                                                    tGCAATTATTCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACGATGATAAGCa         <  2:817044/140‑1 (MQ=255)                                                                                                                           attCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACGATGATAAGCATTCatat  <  1:688940/140‑1 (MQ=255)                                                                                                                           attCGTGCCTTGCTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACGATGATAAGCATTCatat  >  2:1119864/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                CGCCGACAGGCCAGCACGAACGGCTCGTTGACCAGTGGGGTAAAATCAATGGATGAATTTGTGACGTTGTTCATGTTTATGCCAAAGTCTGATTCGTTACACAGCACCGATTCCATGCAATTATTCGTGCCTTGTTCCAGAATCCGCACTTTAATATTGGGATACAGCTCATTAAATTTACCGATTGCCAGCGGTAAAAAATAAAATACTGCGGTCGGAATACACGCTAACGTCACCATACCACGATGATAAGCATTCATAT  >  NC_000913/800058‑800319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑