Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	804,652	A→G	T384A (ACC→GCC)	ybhJ →	putative hydratase YbhJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	804,652	0	A	G	100.0%	139.5 / NA	40	T384A (ACC→GCC)	ybhJ	putative hydratase YbhJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/24);  total (16/24)

AACTCTCGCTGCTGGATAAAGTGGAAAATGGTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACACCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTGATTGGCGCAGGCGCAATCATCAGAACCGCGTTCTGCGGCCCATGCTTTGGCGCGGGC  >  NC_000913/804515‑804786                                                                                                                                          |                                                                                                                                       aaCTCTCGCTGCTGGATAAAGTGGAAAATGGTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGcc                                                                                                                                      >  1:1051050/1‑140 (MQ=255)   ctctCGCTGCTGGATAAAGTGGAAAATGGTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCtt                                                                                                                                    >  1:309891/1‑140 (MQ=255)        gctgctGGATAAAGTGGAAAATGGTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGc                                                                                                                               >  2:992638/1‑140 (MQ=255)        gctgctGGATAAAGTGGAAAATGGTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGc                                                                                                                               <  1:876062/140‑1 (MQ=255)           gctgGATAAAGTGGAAAATGGTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTgg                                                                                                                            <  1:355580/140‑1 (MQ=255)                      tGGAAAATGGTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCg                                                                                                                 <  1:624019/140‑1 (MQ=255)                         aaaaTGGTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGtca                                                                                                              >  1:330311/1‑140 (MQ=255)                            aTGGTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGtcatca                                                                                                           <  1:744395/140‑1 (MQ=255)                               gTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTa                                                                                                                     >  2:625166/1‑127 (MQ=255)                               gTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTa                                                                                                                     <  1:625166/127‑1 (MQ=255)                               gTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAg                                                                                                        <  1:592015/140‑1 (MQ=255)                                tCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGc                                                                                                       >  1:412894/1‑140 (MQ=255)                                 cGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGcc                                                                                                      <  2:1051050/140‑1 (MQ=255)                                 cGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGcc                                                                                                      <  1:992638/140‑1 (MQ=255)                                  gCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCg                                                                                                     >  1:614471/1‑140 (MQ=255)                                  gCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCg                                                                                                     <  2:412894/140‑1 (MQ=255)                                  gCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCg                                                                                                     <  2:614471/140‑1 (MQ=255)                                  gCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCg                                                                                                     <  1:1162614/140‑1 (MQ=255)                                   ccTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCgg                                                                                                    <  1:590139/140‑1 (MQ=255)                                   ccTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCgg                                                                                                    <  2:309891/140‑1 (MQ=255)                                         aGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTa                                                                                              <  1:475970/140‑1 (MQ=255)                                                       ttATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCaaa                                                                                <  2:965284/140‑1 (MQ=255)                                                             cgGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAggg                                                                          >  1:977253/1‑139 (MQ=255)                                                               ggCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGgtgt                                                                        <  2:116470/140‑1 (MQ=255)                                                                 cTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTgg                                                                      <  1:1015257/140‑1 (MQ=255)                                                                 cTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTgg                                                                      <  1:1003507/140‑1 (MQ=255)                                                                  tGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGTCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGt                                                                     <  2:330311/140‑1 (MQ=255)                                                                    ttCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAg                                                                   >  2:1127269/1‑140 (MQ=255)                                                                    ttCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAg                                                                   <  2:463461/140‑1 (MQ=255)                                                                    ttCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAg                                                                   <  1:1127269/140‑1 (MQ=255)                                                                            gTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTg                                                           >  1:622230/1‑140 (MQ=255)                                                                                         cGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTGATTggcgcaggcg                                              <  2:279509/140‑1 (MQ=255)                                                                                           tCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTGATTggcgcaggcgca                                            <  1:693141/140‑1 (MQ=255)                                                                                           tCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTGATTggcgcaggcgca                                            <  2:534438/140‑1 (MQ=255)                                                                                                                   gcgGTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTGATTGGCGCAGGCGCAATCATCAGAACCGCGTTCTGCGGc                    <  1:874810/140‑1 (MQ=255)                                                                                                                      gTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTGATTGGCGCAGGCGCAATCATCAGAACCGCGTTCTGCGGCCCa                 >  2:392297/1‑140 (MQ=255)                                                                                                                      gTCAATCCTGTGGCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTGATTGGCGCAGGCGCAATCATCAGAACCGCGTTCTGCGGCCCa                 >  2:528697/1‑140 (MQ=255)                                                                                                                                 ggCAATGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTGATTGGCGCAGGCGCAATCATCAGAACCGCGTTCTGCGGCCCATGCTTTGgcgc      >  2:188438/1‑140 (MQ=255)                                                                                                                                     aTGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTGATTGGCGCAGGCGCAATCATCAGAACCGCGTTCTGCGGCCCATGCTTTGGCGCGGGc  >  2:347138/1‑140 (MQ=255)                                                                                                                                     aTGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTGATTGGCGCAGGCGCAATCATCAGAACCGCGTTCTGCGGCCCATGCTTTGGCGCGGGc  >  1:1187964/1‑140 (MQ=255)                                                                                                                                     aTGACGCCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTGATTGGCGCAGGCGCAATCATCAGAACCGCGTTCTGCGGCCCATGCTTTGGCGCGGGc  >  1:1059741/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AACTCTCGCTGCTGGATAAAGTGGAAAATGGTCGCCTGAAAGTGCAGCAGGGGATTATCGCGGGCTGTTCTGGCGGTAACTACGAAAACGTCATCGCGGCGGCGAATGCACTGCGCGGTCAATCCTGTGGCAATGACACCTTCTCGCTGGCAGTTTACCCGTCATCACAGCCGGTGTTTATGGATCTCGCCAAAAAAGGTGTGGTAGCAGATTTGATTGGCGCAGGCGCAATCATCAGAACCGCGTTCTGCGGCCCATGCTTTGGCGCGGGC  >  NC_000913/804515‑804786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑