Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	817,768	A→G	Y242C (TAC→TGC)	moaA →	GTP 3',8'‑cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	817,768	0	A	G	100.0%	227.2 / NA	68	Y242C (TAC→TGC)	moaA	GTP 3',8'‑cyclase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/33);  total (35/33)

GCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGC  >  NC_000913/817633‑817902                                                                                                                                        |                                                                                                                                       gCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGcc                                                                                                                                    >  2:1065419/1‑140 (MQ=255) gCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGcc                                                                                                                                    >  2:67756/1‑140 (MQ=255) gCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGcc                                                                                                                                    >  1:686717/1‑140 (MQ=255) gCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGcc                                                                                                                                    >  2:775019/1‑140 (MQ=255)  cGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCg                                                                                                                                   <  2:591717/140‑1 (MQ=255)   gAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCgg                                                                                                                                  <  1:405976/140‑1 (MQ=255)        cAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGgagaga                                                                                                                             <  1:1129241/140‑1 (MQ=255)            gAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTgg                                                                                                                         <  1:108233/140‑1 (MQ=255)            gAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTgg                                                                                                                         <  1:775019/140‑1 (MQ=255)                tctTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCtt                                                                                                                     >  2:1165342/1‑140 (MQ=255)                  ttCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCgg                                                                                                                                  <  2:1144699/125‑1 (MQ=255)                  ttCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCgg                                                                                                                                  >  1:1144699/1‑125 (MQ=255)                   tCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTgg                                                                                                                         >  2:764655/1‑133 (MQ=255)                   tCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTgg                                                                                                                         <  1:764655/133‑1 (MQ=255)                   tCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATc                                                                                                                  <  1:330898/140‑1 (MQ=255)                      gTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATg                                                                                                               <  2:636440/140‑1 (MQ=255)                      gTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATg                                                                                                               <  1:1165342/140‑1 (MQ=255)                            aTCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTAt                                                                                                         >  2:841811/1‑140 (MQ=255)                              cacaTCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCtt                                                                                                                     >  2:926152/1‑126 (MQ=255)                              cacaTCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCtt                                                                                                                     <  1:926152/126‑1 (MQ=255)                              cacaTCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGa                                                                                                       >  1:37308/1‑140 (MQ=255)                                           aGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTgc                                                                                          >  2:156839/1‑140 (MQ=255)                                           aGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTgc                                                                                          >  1:1112072/1‑140 (MQ=255)                                            ggTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTgcg                                                                                         <  1:681994/140‑1 (MQ=255)                                             gTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGaaa                                                                                                     >  2:58642/1‑127 (MQ=255)                                             gTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGaaa                                                                                                     <  1:58642/127‑1 (MQ=255)                                             gTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTgcgc                                                                                        >  1:663505/1‑140 (MQ=255)                                             gTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTgcgc                                                                                        >  1:517559/1‑140 (MQ=255)                                             gTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTgcgc                                                                                        >  2:819414/1‑140 (MQ=255)                                              ttCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGcc                                                                                       <  2:97519/140‑1 (MQ=255)                                                  gCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACtt                                                                                   >  2:302175/1‑140 (MQ=255)                                                       aCGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCAc                                                                                     <  1:908145/133‑1 (MQ=255)                                                       aCGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCAc                                                                                     >  2:908145/1‑133 (MQ=255)                                                        cGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAAcc                                                                             >  1:904134/1‑140 (MQ=255)                                                           gCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGcc                                                                          <  1:709195/140‑1 (MQ=255)                                                             tACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCCCTTGCAACCGCCTg                                                                        <  1:819414/140‑1 (MQ=255)                                                             tACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTg                                                                        <  2:663505/140‑1 (MQ=255)                                                             tACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCACCACTTGCAACCGCCTg                                                                        >  1:703313/1‑140 (MQ=255)                                                               cTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTgcg                                                                      <  1:156839/140‑1 (MQ=255)                                                                tGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTgcgc                                                                     <  1:302175/140‑1 (MQ=255)                                                                   gTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGtt                                                                  <  2:993877/140‑1 (MQ=255)                                                                    tCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTgcgc                                                                     <  1:370355/136‑1 (MQ=255)                                                                    tCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTgcgc                                                                     >  2:370355/1‑136 (MQ=255)                                                                     cggggttGGATCCACAAATCACTTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTCCCATCCAGATTGCGCCGGAGAGATTGGCTTTATCATGCCGTATGAAAAAGCCTTCTGCGCCACTTGCAACCGCCTGCGCGTTtc                                                                <  2:543064/134‑1 (MQ=255)                                                                     cgcgGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTgc                                                                       <  1:1057011/133‑1 (MQ=255)                                                                     cgcgGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTgc                                                                       >  2:1057011/1‑133 (MQ=255)                                                                      gcgGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTtcc                                                               >  1:543064/1‑140 (MQ=255)                                                                         gCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTtcctcc                                                            >  1:417875/1‑140 (MQ=255)                                                                              aTCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTgg                                                       >  2:615269/1‑140 (MQ=255)                                                                               tCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCACCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGt                                                      <  2:703313/140‑1 (MQ=255)                                                                                  accaATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTtcctc                                                             <  1:553189/130‑1 (MQ=255)                                                                                  accaATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTtcctc                                                             >  2:553189/1‑130 (MQ=255)                                                                                    caATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACt                                                 <  1:944491/140‑1 (MQ=255)                                                                                    caATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACt                                                 <  2:179032/140‑1 (MQ=255)                                                                                       ttACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCa                                              >  1:277131/1‑140 (MQ=255)                                                                                        tACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAAc                                                  >  1:837642/1‑135 (MQ=255)                                                                                        tACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAAc                                                  <  2:837642/135‑1 (MQ=255)                                                                                          cGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCAtct                                           >  2:1045670/1‑140 (MQ=255)                                                                                            tCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCGGGAGAGATTGGCCTTATCATGCCGTATG‑AAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTg                                        <  2:1167193/140‑1 (MQ=255)                                                                                             cAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTg                                        >  2:731547/1‑140 (MQ=255)                                                                                                     cGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTg                                >  1:607848/1‑140 (MQ=255)                                                                                                            cccGCGCAAGTCTTTTGCCATCCAGATTGCGCGGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAgg                         <  2:1169509/140‑1 (MQ=255)                                                                                                                     gTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAgg                         >  1:956512/1‑131 (MQ=255)                                                                                                                     gTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAgg                         <  2:956512/131‑1 (MQ=255)                                                                                                                     gTCTTTTGCCATCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTaa                <  2:277131/140‑1 (MQ=255)                                                                                                                                tCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATc     >  2:1139547/1‑140 (MQ=255)                                                                                                                                tCCAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATc     >  2:935452/1‑140 (MQ=255)                                                                                                                                 ccAGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATct    >  2:332659/1‑140 (MQ=255)                                                                                                                                   aGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATctgc  <  2:1112072/140‑1 (MQ=255)                                                                                                                                   aGATTGCGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATctgc  <  1:615269/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GCGAGGGCAGCGAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGCGGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCAGATTACGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGCAACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGCGTTAACCTGCGCGATCTGC  >  NC_000913/817633‑817902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑