Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	846,166	A→G	F100L (TTT→CTT)	glnQ ←	L‑glutamine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	846,166	0	A	G	100.0%	128.9 / NA	37	F100L (TTT→CTT)	glnQ	L‑glutamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/17);  total (20/17)

CGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCC  >  NC_000913/846033‑846298                                                                                                                                      |                                                                                                                                     cGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGAc                                                                                                                                <  2:347584/140‑1 (MQ=255)  gctgctGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACg                                                                                                                               <  2:631910/140‑1 (MQ=255)          tGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAg                                                                                                                       <  1:686427/140‑1 (MQ=255)            accaccAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAgcg                                                                                                                     >  2:681280/1‑140 (MQ=255)                ccaGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGt                                                                                                                 >  1:788889/1‑140 (MQ=255)                 caGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTc                                                                                                                <  2:346020/140‑1 (MQ=255)                  aGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCa                                                                                                               <  2:523790/140‑1 (MQ=255)                           ggAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGa                                                                                                      >  2:339752/1‑140 (MQ=255)                             aaGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAag                                                                                                    >  1:288725/1‑140 (MQ=255)                             aaGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAag                                                                                                    <  1:681280/140‑1 (MQ=255)                                    gTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTaaa                                                                                             >  1:707681/1‑140 (MQ=255)                                        tgtgCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAActg                                                                                         <  2:288725/140‑1 (MQ=255)                                          tgCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAActgct                                                                                       >  2:382887/1‑140 (MQ=255)                                          tgCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAActgct                                                                                       >  1:1213459/1‑140 (MQ=255)                                          tgCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGACGCGGGAAGAGGTAAAActgct                                                                                       >  2:824806/1‑140 (MQ=255)                                            cACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTgg                                                                                     >  2:353388/1‑140 (MQ=255)                                              cGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGaa                                                                                   <  2:492599/140‑1 (MQ=255)                                                 tCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAacac                                                                                >  2:601427/1‑140 (MQ=255)                                                        gACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCt                                                                         <  2:707681/140‑1 (MQ=255)                                                           cGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCt                                                                      >  2:950381/1‑140 (MQ=255)                                                             aCTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTc                                                                    >  1:954054/1‑140 (MQ=255)                                                             aCTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTc                                                                    >  2:794614/1‑140 (MQ=255)                                                                       gcagcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGACGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATc                                                          <  1:824806/140‑1 (MQ=255)                                                                          gcTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAgg                                                       <  1:382887/140‑1 (MQ=255)                                                                                  gCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTc                                               <  1:794614/140‑1 (MQ=255)                                                                                          ttCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCg                                       >  2:156297/1‑140 (MQ=255)                                                                                             cGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGAt                                    <  2:248591/140‑1 (MQ=255)                                                                                                 tcttctTTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGtt                                <  1:339752/140‑1 (MQ=255)                                                                                                    tcttTGTTCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAAc                             <  2:701874/140‑1 (MQ=255)                                                                                                          ttCGCGCCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAg                       >  2:662246/1‑140 (MQ=255)                                                                                                              cgcCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGG‑ACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCAt                  >  1:365782/1‑140 (MQ=255)                                                                                                               gcCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCAt                  >  1:1206764/1‑140 (MQ=255)                                                                                                               gcCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCAt                  >  2:580006/1‑140 (MQ=255)                                                                                                               gcCACGCACGCGTAGCGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCAt                  <  1:662246/140‑1 (MQ=255)                                                                                                                              cGGGCCAAGCATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGc   >  1:365082/1‑140 (MQ=255)                                                                                                                                  ccAAGCATCACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGcc  >  1:184267/1‑137 (MQ=255)                                                                                                                                  ccAAGCATCACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGcc  <  2:184267/137‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     CGCTGCTGTTGACCACCAGAAAGTTCGGAAGGGTAGTGATGTGCACGTTCTGCCAGACCGACTTTCGCCAGCAGCTCACGTGCCAGTTTTTCCGCCTCTTCTTTGTTCGCGCCACGCACGCGTAGCGGGCCAAACATGACGTTTTCCAGCGCTGTCAGATGCGGGAAGAGGTAAAACTGCTGGAACACCATACCTGCTTCCTGGCGAATCAGGCGCTCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCC  >  NC_000913/846033‑846298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑