Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 880,928 A→G T68A (ACT→GCT)  dacC → D‑alanyl‑D‑alanine carboxypeptidase DacC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913880,9280AG98.2% 188.1 / NA 54T68A (ACT→GCT) dacCD‑alanyl‑D‑alanine carboxypeptidase DacC
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (18/35);  total (18/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAT  >  NC_000913/880791‑881058
                                                                                                                                         |                                                                                                                                  
ccccAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCt                                                                                                                                  >  2:998092/1‑140 (MQ=255)
 cccAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTa                                                                                                                                 <  1:862146/140‑1 (MQ=255)
 cccAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTa                                                                                                                                 <  1:1043883/140‑1 (MQ=255)
      cGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATc                                                                                                                            <  1:793403/140‑1 (MQ=255)
        gCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCAt                                                                                                                          <  1:700528/140‑1 (MQ=255)
        gCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCAt                                                                                                                          <  2:856670/140‑1 (MQ=255)
           ttCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGAc                                                                                                                       <  1:1149685/140‑1 (MQ=255)
           ttCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGAc                                                                                                                       >  1:1189435/1‑140 (MQ=255)
            tCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGAcc                                                                                                                      <  1:824146/140‑1 (MQ=255)
            tCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGAcc                                                                                                                      <  2:485837/140‑1 (MQ=255)
              gcggcgGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAg                                                                                                                    <  2:944696/140‑1 (MQ=255)
                    gAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTAtgt                                                                                                              >  2:1147143/1‑140 (MQ=255)
                      aCAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTgg                                                                                                            <  2:719575/140‑1 (MQ=255)
                       cAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGt                                                                                                           <  2:1189435/140‑1 (MQ=255)
                               tGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAg                                                                                                    >  2:1206915/1‑139 (MQ=255)
                               tGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAg                                                                                                    <  1:1206915/139‑1 (MQ=255)
                                   gcgcCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCt                                                                                               <  1:108398/140‑1 (MQ=255)
                                      ccGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTaa                                                                                            >  2:181723/1‑140 (MQ=255)
                                      ccGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTaa                                                                                            <  1:1147143/140‑1 (MQ=255)
                                      ccGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTaa                                                                                            <  2:975551/140‑1 (MQ=255)
                                      ccGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTaa                                                                                            >  1:121056/1‑140 (MQ=255)
                                      ccGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTaa                                                                                            >  1:471068/1‑140 (MQ=255)
                                               gATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATaa                                                                                   >  1:1153835/1‑140 (MQ=255)
                                                 tGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGa                                                                                 <  2:955732/140‑1 (MQ=255)
                                                  gcgcGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGAt                                                                                <  2:538143/140‑1 (MQ=255)
                                                   cgcgTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGATACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGAtt                                                                               <  2:1056029/140‑1 (MQ=255)
                                                   cgcgTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGAtt                                                                               <  2:308203/140‑1 (MQ=255)
                                                    gcgTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTa                                                                              <  2:121056/140‑1 (MQ=255)
                                                      gTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTaaa                                                                            <  1:801716/140‑1 (MQ=255)
                                                         cATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTc                                                                         >  2:947391/1‑140 (MQ=255)
                                                           tGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCAc                                                                       <  1:59652/140‑1 (MQ=255)
                                                           tGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCAc                                                                       <  1:181723/140‑1 (MQ=255)
                                                                  tAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATg                                                                >  1:229275/1‑140 (MQ=255)
                                                                        aTTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGGGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGAcg                                                          <  1:17137/140‑1 (MQ=255)
                                                                        aTTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGGcg                                                          >  2:610337/1‑140 (MQ=255)
                                                                        aTTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGAcg                                                          <  1:628148/140‑1 (MQ=255)
                                                                        aTTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGAcg                                                          <  1:153894/140‑1 (MQ=255)
                                                                        aTTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGAcg                                                          >  2:1000663/1‑140 (MQ=255)
                                                                          tACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGa                                                                    <  1:369525/128‑1 (MQ=255)
                                                                          tACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGa                                                                    >  2:369525/1‑128 (MQ=255)
                                                                          tACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGAcggt                                                        <  1:317276/140‑1 (MQ=255)
                                                                            cGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGAcggtcg                                                      >  1:556426/1‑140 (MQ=255)
                                                                            cGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGAcggtcg                                                      <  1:1186742/140‑1 (MQ=255)
                                                                                       aaGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGcc                                           >  1:505529/1‑140 (MQ=255)
                                                                                               gCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGAc                                   <  2:229275/140‑1 (MQ=255)
                                                                                                         aTGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCg                         >  2:987891/1‑140 (MQ=255)
                                                                                                          tGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCgg                        <  2:176295/140‑1 (MQ=255)
                                                                                                          tGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCgg                        >  1:120819/1‑140 (MQ=255)
                                                                                                          tGCGGATGAGAAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCgg                        <  1:947391/140‑1 (MQ=255)
                                                                                                                 gagaAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCg                 <  1:998092/140‑1 (MQ=255)
                                                                                                                 gagaAACTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCg                 <  2:120819/140‑1 (MQ=255)
                                                                                                                    aaaCTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTgg              <  1:531844/140‑1 (MQ=255)
                                                                                                                    aaaCTGGATCCCGCGAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTgg              <  1:1000663/140‑1 (MQ=255)
                                                                                                                                gcgAGCCTGGCTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAt  >  1:897498/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                  
CCCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAT  >  NC_000913/880791‑881058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑