Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	51,317	C→T	G97D (GGT→GAT)	apaG ←	DUF525 domain‑containing protein ApaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	51,317	0	C	T	100.0%	280.7 / NA	79	G97D (GGT→GAT)	apaG	DUF525 domain‑containing protein ApaG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/45);  total (34/45)

ATCGTAACAACCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGAT  >  NC_000913/51181‑51453                                                                                                                                         |                                                                                                                                         aTCGTAACAACCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCt                                                                                                                                       <  1:1059213/140‑1 (MQ=255) aTCGTAACAACCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCt                                                                                                                                       <  1:862041/140‑1 (MQ=255) aTCGTAACAACCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCt                                                                                                                                       >  1:964079/1‑140 (MQ=255)    gTAACAACCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCa                                                                                                                                    <  1:389261/140‑1 (MQ=255)    gTAACAACCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCa                                                                                                                                    <  2:860294/140‑1 (MQ=255)    gTAACAACCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCa                                                                                                                                    <  1:1096280/140‑1 (MQ=255)      aacaacCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATg                                                                                                                                  >  1:431258/1‑140 (MQ=255)      aacaacCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATg                                                                                                                                  >  1:713328/1‑140 (MQ=255)        caacCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGt                                                                                                                                >  1:550304/1‑140 (MQ=255)          acCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGc                                                                                                                              >  2:670787/1‑140 (MQ=255)          acCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGc                                                                                                                              >  2:339559/1‑140 (MQ=255)           ccttgcaCGTCGCCAATACCGGATGTGGCCCTATTCTTTTAATGGGTGTTGGTGGCACCGGCGCGTCGGAATAGGTGGATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGcc                                                                                                                             >  2:749227/7‑140 (MQ=255)           ccATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGcc                                                                                                                             <  1:1079017/140‑1 (MQ=255)            cATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGccc                                                                                                                            >  2:297525/1‑140 (MQ=255)             aTGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCa                                                                                                                           >  2:496101/1‑140 (MQ=255)              tGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAg                                                                                                                          <  1:447590/140‑1 (MQ=255)                aaCGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCg                                                                                                                        <  2:1179248/140‑1 (MQ=255)                  cGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCggg                                                                                                                      <  2:1137009/140‑1 (MQ=255)                  cGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCggg                                                                                                                      <  1:496101/140‑1 (MQ=255)                   gTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCgggg                                                                                                                     >  1:322556/1‑140 (MQ=255)                    tCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGACATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGt                                                                                                                    >  1:1030740/1‑140 (MQ=255)                      gCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGttt                                                                                                                  <  1:646778/140‑1 (MQ=255)                       ccAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTc                                                                                                                 <  1:339559/140‑1 (MQ=255)                       ccAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTc                                                                                                                 <  1:670787/140‑1 (MQ=255)                       ccAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTc                                                                                                                 <  1:235746/140‑1 (MQ=255)                          aTAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAAt                                                                                                              <  1:108041/140‑1 (MQ=255)                               gTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTg                                                                                                         <  2:431258/140‑1 (MQ=255)                                  tGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCAc                                                                                                      >  2:214619/1‑140 (MQ=255)                                     cGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGc                                                                                                   <  1:388327/140‑1 (MQ=255)                                      gCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCt                                                                                                  <  1:1168188/140‑1 (MQ=255)                                      gCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCt                                                                                                  <  1:1096632/140‑1 (MQ=255)                                       ccATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTg                                                                                                 >  2:243319/1‑140 (MQ=255)                                       ccATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTg                                                                                                 >  2:316034/1‑140 (MQ=255)                                        cATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTc                                                                                                                 <  2:1162610/123‑1 (MQ=255)                                        cATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTc                                                                                                                 >  1:1162610/1‑123 (MQ=255)                                        cATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTgg                                                                                                <  2:767704/140‑1 (MQ=255)                                        cATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTgg                                                                                                <  2:713328/140‑1 (MQ=255)                                         atatTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGgt                                                                                               >  1:1182911/1‑140 (MQ=255)                                          tatTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGgtg                                                                                              <  1:465191/140‑1 (MQ=255)                                                         tgaGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCg                                                                               >  2:231085/1‑140 (MQ=255)                                                         tgaGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCg                                                                               >  1:446814/1‑140 (MQ=255)                                                         tgaGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCg                                                                               >  1:722930/1‑140 (MQ=255)                                                         tgaGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCg                                                                               >  1:1019689/1‑140 (MQ=255)                                                           aGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGcc                                                                             >  2:677681/1‑140 (MQ=255)                                                            gtgtGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCa                                                                            <  1:1177689/140‑1 (MQ=255)                                                               tgGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGgc                                                                         >  1:554336/1‑140 (MQ=255)                                                                 ggAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGgcgc                                                                       >  1:530579/1‑140 (MQ=255)                                                                 ggAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGgcgc                                                                       >  2:1140794/1‑140 (MQ=255)                                                                  gAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGcgcg                                                                      >  1:1150247/1‑140 (MQ=255)                                                                    aCGGCGAGTCGGAATATGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGAt                                                                    <  1:978882/140‑1 (MQ=255)                                                                    aCGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGAt                                                                    <  1:721204/140‑1 (MQ=255)                                                                     cGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATa                                                                   <  2:950703/140‑1 (MQ=255)                                                                     cGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATa                                                                   <  1:243319/140‑1 (MQ=255)                                                                       gCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAg                                                                 >  2:603861/1‑140 (MQ=255)                                                                       gCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAg                                                                 <  2:170161/140‑1 (MQ=255)                                                                         gAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTg                                                               >  2:660317/1‑140 (MQ=255)                                                                                   aCGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGcc                                                     <  2:722930/140‑1 (MQ=255)                                                                                     gggAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCaa                                                   >  2:1080558/1‑140 (MQ=255)                                                                                     gggAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCaa                                                   >  1:451810/1‑140 (MQ=255)                                                                                        aaTGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCa                                                <  2:451810/140‑1 (MQ=255)                                                                                         aTGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCAc                                               >  1:1203313/1‑140 (MQ=255)                                                                                          tGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACt                                              >  1:456778/1‑140 (MQ=255)                                                                                           gTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTc                                             >  1:499767/1‑140 (MQ=255)                                                                                             cGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCt                                           <  2:964079/140‑1 (MQ=255)                                                                                                      aaGGGACGCCATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTgg                                  >  2:670777/1‑140 (MQ=255)                                                                                                              ccATTTTCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGt                          >  2:647652/1‑140 (MQ=255)                                                                                                                  tttCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCa                      <  1:214619/140‑1 (MQ=255)                                                                                                                  tttCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCa                      <  1:647652/140‑1 (MQ=255)                                                                                                                  tttCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCa                      <  2:1162856/140‑1 (MQ=255)                                                                                                                  tttCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCa                      <  1:670777/140‑1 (MQ=255)                                                                                                                  tttCATCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCa                      <  2:1019689/140‑1 (MQ=255)                                                                                                                       tCGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgcc                 <  1:725504/140‑1 (MQ=255)                                                                                                                       gcGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTACAATGATTGCCCCGCTGGTGTACTGGTACTCTGCGCCAGGGGCGATCAGTGGGTGGACGCCATCCTCTCCTTCGCCCTGGACTTCGGTTTCACgggc                 <  2:1025587/139‑3 (MQ=255)                                                                                                                        cGATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgcca                <  2:925471/140‑1 (MQ=255)                                                                                                                         gATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccat               <  2:456778/140‑1 (MQ=255)                                                                                                                         gATCATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccat               >  2:1205838/1‑140 (MQ=255)                                                                                                                            cATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccattgc            <  2:1203313/140‑1 (MQ=255)                                                                                                                            cATTTCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccattgc            <  2:446814/140‑1 (MQ=255)                                                                                                                              tttCGTAGTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccattgcca          <  1:1080558/140‑1 (MQ=255)                                                                                                                                     gTGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGa   <  2:1030740/140‑1 (MQ=255)                                                                                                                                      tGATCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGAt  <  1:677681/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         ATCGTAACAACCATGAACGTCGCCAATAAGGTATGTCGCCATATTCTTTTAATGAATGAGTGTGGGAACGGCGAGTCGGAATACGGGAATGTCGATGCTGAAAGGGACGCCATTTTCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGAT  >  NC_000913/51181‑51453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑