Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	964,293	A→G	intergenic (+181/‑27)	ihfB → / → ycaI	integration host factor subunit beta/conserved inner membrane protein YcaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	964,293	0	A	G	100.0%	136.6 / NA	39	intergenic (+181/‑27)	ihfB/ycaI	integration host factor subunit beta/conserved inner membrane protein YcaI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/21);  total (18/21)

CTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTGGGACATTAACCCTTGCGTTTCTGACTCTCTTCGC  >  NC_000913/964157‑964426                                                                                                                                         |                                                                                                                                      cTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCgcac                                                                                                                                    <  1:1017790/140‑1 (MQ=255) cTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCgcac                                                                                                                                    <  1:1145151/140‑1 (MQ=255) cTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCgcac                                                                                                                                    <  2:25200/140‑1 (MQ=255) cTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCgcac                                                                                                                                    >  2:107348/1‑140 (MQ=255) cTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCgcac                                                                                                                                    <  1:73624/140‑1 (MQ=255) cTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCgcac                                                                                                                                    <  1:731014/140‑1 (MQ=255) cTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCgcac                                                                                                                                    <  1:598969/140‑1 (MQ=255)  ttttttCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACt                                                                                                                                   <  1:482172/140‑1 (MQ=255)      ttCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTcc                                                                                                                               <  2:568668/140‑1 (MQ=255)          tttCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATaa                                                                                                                           <  1:648284/140‑1 (MQ=255)           ttCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATaaa                                                                                                                          >  2:538692/1‑140 (MQ=255)                tAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACaggg                                                                                                                     >  2:167424/1‑140 (MQ=255)                  aTCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACagggag                                                                                                                   <  2:1185417/140‑1 (MQ=255)                     tggaactggaaGCTGCCTCACAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAgggg                                                                                                                >  2:956056/1‑140 (MQ=255)                       gaactggaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTg                                                                                                              >  2:879131/1‑140 (MQ=255)                           tggaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATg                                                                                                          <  1:1039286/140‑1 (MQ=255)                            ggaaGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGa                                                                                                         >  1:396556/1‑140 (MQ=255)                               aGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGaaaa                                                                                                      >  1:724246/1‑140 (MQ=255)                                     cTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGa                                                                                                >  2:926313/1‑140 (MQ=255)                                     cTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGa                                                                                                <  2:1051135/140‑1 (MQ=255)                                       cGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACa                                                                                              >  1:474129/1‑140 (MQ=255)                                         cAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt                                                                                            <  1:956056/140‑1 (MQ=255)                                         cAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt                                                                                            <  2:1066643/140‑1 (MQ=255)                                         cAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGt                                                                                            <  2:923488/140‑1 (MQ=255)                                            agTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCgg                                                                                         <  1:926313/140‑1 (MQ=255)                                             gTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGgt                                                                                        <  1:879131/140‑1 (MQ=255)                                             gTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGgt                                                                                        <  2:225547/140‑1 (MQ=255)                                              ttttGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGgtg                                                                                       <  1:316094/140‑1 (MQ=255)                                                      aGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATa                                                                               >  2:18719/1‑140 (MQ=255)                                                        ttttCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAAt                                                                             >  2:922074/1‑140 (MQ=255)                                                                  ttCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAAtt                                                                   >  1:1138127/1‑140 (MQ=255)                                                                      ttAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCg                                                                        >  1:249807/1‑131 (MQ=255)                                                                      ttAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCg                                                                        <  2:249807/131‑1 (MQ=255)                                                                                     tatGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGc                                                <  1:18719/140‑1 (MQ=255)                                                                                        gCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGcccc                                             >  2:432439/1‑140 (MQ=255)                                                                                        gCCTCCGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGcccc                                             >  1:323639/1‑140 (MQ=255)                                                                                          cTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCaa                                           >  1:289865/1‑140 (MQ=255)                                                                                             ggggAACGCAAAATTCCCACCAGCCAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTg                                                          >  2:666864/1‑122 (MQ=255)                                                                                             ggggAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTCCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTg                                                          <  1:666864/122‑1 (MQ=255)                                                                                                 aaCGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTg                                    <  1:107348/140‑1 (MQ=255)                                                                                                  aCGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTgg                                   <  2:1197855/140‑1 (MQ=255)                                                                                                   cGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTggg                                  >  1:845320/1‑140 (MQ=255)                                                                                                         aTTCCCACCAGACAACCGCTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTGGGACATTa                            >  2:187735/1‑140 (MQ=255)                                                                                                                           cTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTGGGACATTAACCCTTGCGTTTCTGAct          <  2:323639/140‑1 (MQ=255)                                                                                                                           cTCAACAAAGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTGGGACATTAACCCTTGCGTTTCTGAct          >  2:834753/1‑140 (MQ=255)                                                                                                                                   aGTTGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTGGGACATTAACCCTTGCGTTTCTGACTCTCTTCGc  <  1:363889/140‑1 (MQ=255)                                                                                                                                      tGCGCACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTGGGACATTAACCCTTGCGTTTCTGACTCTCTTCGc  <  2:1147395/137‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      CTTTTTTCATTTCTCTAATCTGGAACTGGAAGCTGCCTCGCAGAGTTTTGAACAGTTTTCACCCTTTCGTTAAATTCTTCTGAATATGCCTCGGGGAACGCAAAATTCCCACCAGACAACCGCTCAACAAAGTTGCACACTTTCCATAAACAGGGAGGGGTGCATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTGGGACATTAACCCTTGCGTTTCTGACTCTCTTCGC  >  NC_000913/964157‑964426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑