Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,035,913	A→G	G48G (GGA→GGG)	hyaD →	putative hydrogenase 1 maturation protease HyaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,035,913	0	A	G	100.0%	146.8 / NA	42	G48G (GGA→GGG)	hyaD	putative hydrogenase 1 maturation protease HyaD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/23);  total (19/23)

AGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATG  >  NC_000913/1035777‑1036048                                                                                                                                         |                                                                                                                                        agcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTg                                                                                                                                      >  2:119507/1‑140 (MQ=255)   cAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGaa                                                                                                                                    <  2:1044330/140‑1 (MQ=255)   cAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGaa                                                                                                                                    <  1:351306/140‑1 (MQ=255)       gcgTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTg                                                                                                                                <  1:47231/140‑1 (MQ=255)        cgTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGc                                                                                                                               <  2:589698/140‑1 (MQ=255)            gtggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAAc                                                                                                                                   >  1:1033643/1‑132 (MQ=255)            gtggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAAc                                                                                                                                   <  2:1033643/132‑1 (MQ=255)            gtggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTggg                                                                                                                           >  1:505440/1‑140 (MQ=255)              ggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGt                                                                                                                         <  2:321858/140‑1 (MQ=255)                       gCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGaaa                                                                                                                >  1:632084/1‑140 (MQ=255)                        cTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAg                                                                                                               <  2:632084/140‑1 (MQ=255)                         tGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAgc                                                                                                              >  2:112946/1‑140 (MQ=255)                          gggCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAgcg                                                                                                             <  2:505440/140‑1 (MQ=255)                           ggCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGGACTTGCTGGGGTATGTCGAAAgcgc                                                                                                            >  1:800828/1‑140 (MQ=255)                             cAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCgcca                                                                                                          <  2:429535/140‑1 (MQ=255)                              aaCCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCgccag                                                                                                         <  1:119507/140‑1 (MQ=255)                                ccTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCgccagcc                                                                                                       >  2:239257/1‑140 (MQ=255)                                 ctgctgTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAgcgc                                                                                                            <  2:243657/134‑1 (MQ=255)                                 ctgctgTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAgcgc                                                                                                            >  1:243657/1‑134 (MQ=255)                                      gtgGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGt                                                                                                 >  2:925625/1‑140 (MQ=255)                                          gCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGAt                                                                                             >  2:1168052/1‑140 (MQ=255)                                                   ggCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGc                                                                                    <  1:925625/140‑1 (MQ=255)                                                            gTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTa                                                                           <  1:538371/140‑1 (MQ=255)                                                                                             cACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTAtac                                          >  1:634414/1‑138 (MQ=255)                                                                                                tGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCgg                                       <  2:467562/140‑1 (MQ=255)                                                                                                    ccGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAAc                                                >  2:930864/1‑127 (MQ=255)                                                                                                    ccGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAAc                                                <  1:930864/127‑1 (MQ=255)                                                                                                    ccGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGaa                                   >  1:628567/1‑140 (MQ=255)                                                                                                    ccGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGaa                                   <  2:67415/140‑1 (MQ=255)                                                                                                      gAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACg                                 >  1:1207358/1‑140 (MQ=255)                                                                                                         tATGTGGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCAt                              <  2:1207358/140‑1 (MQ=255)                                                                                                           tgtgGAGATTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATACCGGAGAACGCATTc                            <  2:634414/140‑1 (MQ=255)                                                                                                                 gaTTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCtt                      >  1:359746/1‑140 (MQ=255)                                                                                                                  aTTGTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTa                     >  1:748922/1‑140 (MQ=255)                                                                                                                     gTCGATGGCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtct                  <  2:628567/140‑1 (MQ=255)                                                                                                                            gCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTc                            >  2:1167553/1‑123 (MQ=255)                                                                                                                            gCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTc                            <  1:1167553/123‑1 (MQ=255)                                                                                                                            gCGGTACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGcgcg           <  1:1168052/140‑1 (MQ=255)                                                                                                                                tACTCAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCgaaga       >  1:1149883/1‑140 (MQ=255)                                                                                                                                    cAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAAt   <  2:1109005/140‑1 (MQ=255)                                                                                                                                    cAGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAAt   <  2:88670/140‑1 (MQ=255)                                                                                                                                     aGGGGCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATg  >  1:1082086/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATG  >  NC_000913/1035777‑1036048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑