Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,069,275	A→G	F189L (TTT→CTT)	rutG ←	pyrimidine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,069,275	0	A	G	100.0%	145.8 / NA	42	F189L (TTT→CTT)	rutG	pyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/19);  total (23/19)

AACCAGGCAGCATGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTCACCACC  >  NC_000913/1069139‑1069408                                                                                                                                         |                                                                                                                                      aaCCAGGCAGCATGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAgcac                                                                                                                                    <  1:416705/140‑1 (MQ=255)       gcagcaTGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGccac                                                                                                                              >  1:52790/1‑140 (MQ=255)         agcaTGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCATGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGccacca                                                                                                                            <  1:52765/140‑1 (MQ=255)           caTGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGccac                                                                                                                              >  1:1041100/1‑136 (MQ=255)           caTGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGccac                                                                                                                              <  2:1041100/136‑1 (MQ=255)           caTGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGc                                                                                                                          >  1:330187/1‑140 (MQ=255)            aTGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGcc                                                                                                                         <  2:1207388/140‑1 (MQ=255)            aTGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGcc                                                                                                                         <  2:722134/140‑1 (MQ=255)             tGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGccc                                                                                                                        >  2:318353/1‑140 (MQ=255)              ggCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCa                                                                                                                       <  1:543287/140‑1 (MQ=255)                cTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAAt                                                                                                                     >  1:350326/1‑140 (MQ=255)                     gagaGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATAcaca                                                                                                                >  1:363099/1‑140 (MQ=255)                          tAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCAcc                                                                                                           >  2:271914/1‑140 (MQ=255)                                 gACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTa                                                                                                    >  1:1200063/1‑140 (MQ=255)                                  aCGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTAc                                                                                                   <  1:271914/140‑1 (MQ=255)                                  aCGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTAc                                                                                                   >  1:924554/1‑140 (MQ=255)                                    ggCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCg                                                                                                 <  2:937837/140‑1 (MQ=255)                                    ggCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCg                                                                                                 <  2:425426/140‑1 (MQ=255)                                            ccAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAg                                                                                         <  2:239884/140‑1 (MQ=255)                                                     aaaCGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAg                                                                                >  2:76585/1‑140 (MQ=255)                                                          ttGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGa                                                                           <  2:330187/140‑1 (MQ=255)                                                           tGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGaa                                                                          >  2:832785/1‑140 (MQ=255)                                                             gTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGc                                                                        >  1:746842/1‑140 (MQ=255)                                                                           cagcagACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCa                                                          <  2:350326/140‑1 (MQ=255)                                                                            agcagACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCAc                                                         <  1:803316/140‑1 (MQ=255)                                                                            agcagACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGACCGAAGCCGATACACTTTTCAc                                                         >  2:1103114/1‑140 (MQ=255)                                                                                     gCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAgg                                                >  1:1111594/1‑140 (MQ=255)                                                                                      cGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGgc                                               >  2:1179748/1‑140 (MQ=255)                                                                                         cAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCt                                            <  2:1111594/140‑1 (MQ=255)                                                                                              aaTCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGtt                                       <  2:589424/140‑1 (MQ=255)                                                                                                    aCGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGcc                                 >  1:212475/1‑140 (MQ=255)                                                                                                     cGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGc                                  <  1:873585/138‑1 (MQ=255)                                                                                                     cGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGc                                  >  2:873585/1‑138 (MQ=255)                                                                                                     cGAGAATCAATAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCa                                >  2:767671/1‑140 (MQ=255)                                                                                                             aaTAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCAt                        <  2:95542/140‑1 (MQ=255)                                                                                                               tAACCGCTGGATCATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATca                      >  1:763620/1‑140 (MQ=255)                                                                                                                           cATGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCg          <  2:363099/140‑1 (MQ=255)                                                                                                                             tGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCg                <  2:548432/132‑1 (MQ=255)                                                                                                                             tGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCg                >  1:548432/1‑132 (MQ=255)                                                                                                                             tGCCACGGGTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTc        >  1:1118262/1‑140 (MQ=255)                                                                                                                                   gggTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTCaccacc  >  1:195710/1‑140 (MQ=255)                                                                                                                                     gTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTcac      <  1:916597/134‑1 (MQ=255)                                                                                                                                     gTAAGCACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTcac      >  2:916597/1‑134 (MQ=255)                                                                                                                                         |                                                                                                                                      AACCAGGCAGCATGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTCACCACC  >  NC_000913/1069139‑1069408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑