Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,125,779 G→C G73A (GGT→GCT)  rimJ → ribosomal‑protein‑S5‑alanine N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,125,7790GC96.2% 182.5 / 0.2 53G73A (GGT→GCT) rimJribosomal‑protein‑S5‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base C (28/23);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

GGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCA  >  NC_000913/1125647‑1125908
                                                                                                                                    |                                                                                                                                 
ggCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCt                                                                                                                            >  1:64749/1‑140 (MQ=255)
  cGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCttt                                                                                                                          <  2:1168149/140‑1 (MQ=255)
   gTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTc                                                                                                                         >  2:659844/1‑140 (MQ=255)
      ttGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTAc                                                                                                                      >  2:185427/1‑140 (MQ=255)
                ttaCGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTAt                                                                                                            <  2:241055/140‑1 (MQ=255)
                ttaCGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTAt                                                                                                            >  1:369645/1‑140 (MQ=255)
                ttaCGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTAt                                                                                                            <  1:1037150/140‑1 (MQ=255)
                  aCGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTc                                                                                                          >  2:527233/1‑140 (MQ=255)
                        agaATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCg                                                                                                    >  1:1060475/1‑140 (MQ=255)
                          aaTCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGa                                                                                                  >  2:41816/1‑140 (MQ=255)
                          aaTCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGa                                                                                                  <  2:369645/140‑1 (MQ=255)
                          aaTCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGa                                                                                                  <  1:185427/140‑1 (MQ=255)
                          aaTCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGa                                                                                                  >  1:683931/1‑140 (MQ=255)
                             cGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTa                                                                                                             <  2:608075/126‑1 (MQ=255)
                             cGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTa                                                                                                             >  1:608075/1‑126 (MQ=255)
                               ccATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGaaa                                                                                             >  1:894950/1‑140 (MQ=255)
                                cATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGaaaa                                                                                            <  1:475164/140‑1 (MQ=255)
                                 aTTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGaaaaa                                                                                           <  1:659844/140‑1 (MQ=255)
                                           gCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTg                                                                                 <  1:1641/140‑1 (MQ=255)
                                                  gAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGc                                                                          <  1:390920/140‑1 (MQ=255)
                                                    gCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCa                                                                        >  1:108643/1‑140 (MQ=255)
                                                     ccAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCaa                                                                       >  2:506054/1‑140 (MQ=255)
                                                      cAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAAt                                                                      <  2:301711/140‑1 (MQ=255)
                                                      cAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAAt                                                                      <  2:683931/140‑1 (MQ=255)
                                                        gTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAAttt                                                                    <  2:894950/140‑1 (MQ=255)
                                                           cgcgACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTc                                                                 >  2:876390/1‑140 (MQ=255)
                                                           cgcgACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTc                                                                 <  1:1154195/140‑1 (MQ=255)
                                                            gcgACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTcc                                                                >  2:877549/1‑140 (MQ=255)
                                                             cgacgaAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCa                                                               >  2:167057/1‑140 (MQ=255)
                                                                cgaAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAAtg                                                            <  2:1060475/140‑1 (MQ=255)
                                                                 gaAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAAtgt                                                           <  1:877549/140‑1 (MQ=255)
                                                                   aaGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTAAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCAACTTTTTTCAAtgttg                                                         >  2:134746/1‑140 (MQ=255)
                                                                       cACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCg                                                     <  1:1145528/140‑1 (MQ=255)
                                                                            ttATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGct                                                <  1:629050/140‑1 (MQ=255)
                                                                                  aTCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTc                                          >  2:770216/1‑140 (MQ=255)
                                                                                    cAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCAt                                        >  1:852452/1‑140 (MQ=255)
                                                                                    cAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCAt                                        >  1:512158/1‑140 (MQ=255)
                                                                                      ggCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGc                                      >  1:937283/1‑140 (MQ=255)
                                                                                      ggCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCtttt                                           >  1:462985/1‑135 (MQ=255)
                                                                                      ggCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCtttt                                           <  2:462985/135‑1 (MQ=255)
                                                                                      ggCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGc                                      >  2:947817/1‑140 (MQ=255)
                                                                                              ggccaggcTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTAtc                              <  1:467107/140‑1 (MQ=255)
                                                                                               gccaggcTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTAtct                             <  2:937283/140‑1 (MQ=255)
                                                                                               gccaggcTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTAtct                             >  1:406304/1‑140 (MQ=255)
                                                                                                     cTGGGGATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTa                       >  1:12166/1‑140 (MQ=255)
                                                                                                         ggATGATTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACAAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCg                   >  2:1083202/1‑140 (MQ=255)
                                                                                                          gatgatTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGa                  <  1:527233/140‑1 (MQ=255)
                                                                                                              atTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTgg              >  2:166462/1‑140 (MQ=255)
                                                                                                              atTAACGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTgg              >  1:540132/1‑140 (MQ=255)
                                                                                                                   cGAATTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCaaa         <  1:41816/140‑1 (MQ=255)
                                                                                                                     aaTTTCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCaaaaa       >  2:850254/1‑140 (MQ=255)
                                                                                                                        ttCATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATgg    >  1:1204020/1‑140 (MQ=255)
                                                                                                                          cATAAACAAGCTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCa  >  2:607587/1‑140 (MQ=255)
                                                                                                                          cATAAACAAGCTTCAGCTTTCTACTTCGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCa  <  1:850254/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                 
GGCGTCTTGCGGATTATTACGCAGAGAATCGCCATTTCCTCAAGCCCTGGGAGCCAGTGCGCGACGAAAGCCACTGTTATCCATCAGGCTGGCAGGCCAGGCTGGGGATGATTAACGAATTTCATAAACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCA  >  NC_000913/1125647‑1125908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑