Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,176,499	T→C	V358A (GTG→GCG)	lolC →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,176,499	0	T	C	100.0%	170.8 / NA	50	V358A (GTG→GCG)	lolC	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/28);  total (22/28)

TTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAATAAGATC  >  NC_000913/1176366‑1176630                                                                                                                                      |                                                                                                                                    tttATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGTGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTAt                                                                                                                               >  2:90868/1‑140 (MQ=255) tttATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTAt                                                                                                                               <  1:78148/140‑1 (MQ=255)    aTGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGa                                                                                                                            <  2:842426/140‑1 (MQ=255)    aTGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGa                                                                                                                            <  1:662174/140‑1 (MQ=255)    aTGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGa                                                                                                                            <  1:417171/140‑1 (MQ=255)     tGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGaa                                                                                                                           <  1:1042011/140‑1 (MQ=255)     tGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGaa                                                                                                                           <  2:1207659/140‑1 (MQ=255)        tGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTAt                                                                                                                               >  2:776809/1‑133 (MQ=255)        tGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTAt                                                                                                                               <  1:776809/133‑1 (MQ=255)        tGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCt                                                                                                                        >  1:8952/1‑140 (MQ=255)        tGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCt                                                                                                                        >  1:187092/1‑140 (MQ=255)          cAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCttt                                                                                                                      >  2:222761/1‑140 (MQ=255)             ggggCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACa                                                                                                                   >  2:539460/1‑140 (MQ=255)              gggCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAg                                                                                                                  <  2:71782/140‑1 (MQ=255)               ggCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAgg                                                                                                                 >  2:593968/1‑140 (MQ=255)                gCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGt                                                                                                                <  2:719970/140‑1 (MQ=255)                  cAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCa                                                                                                              >  1:659692/1‑140 (MQ=255)                                    tGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCtggtgg                                                                                            >  1:13501/1‑140 (MQ=255)                                                   ggcgCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCgcgc                                                                             <  2:948899/140‑1 (MQ=255)                                                     cgcTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCgctg                                                                           <  1:573840/140‑1 (MQ=255)                                                     cgcTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCgctg                                                                           >  1:1159709/1‑140 (MQ=255)                                                     cgcTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCgctg                                                                           <  1:241246/140‑1 (MQ=255)                                                     cgcTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCgctg                                                                           <  2:624746/140‑1 (MQ=255)                                                     cgcTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCCTTACAGGTCATTGTTATTGCGCTGGTGGCGGTGGCTATCGCgctg                                                                           >  1:550936/1‑140 (MQ=255)                                                          ggCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTc                                                                      >  1:819076/1‑140 (MQ=255)                                                           gcgcCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCt                                                                     <  1:255019/140‑1 (MQ=255)                                                              cccTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGTGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACg                                                                  <  1:1178085/140‑1 (MQ=255)                                                                  gCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCttt                                                              <  1:479204/140‑1 (MQ=255)                                                                         agccagTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTc                                                       >  2:974965/1‑140 (MQ=255)                                                                                    taatCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTg                                            <  1:593968/140‑1 (MQ=255)                                                                                     aatCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTgc                                           >  2:658614/1‑140 (MQ=255)                                                                                            tGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACt                                    >  2:22187/1‑140 (MQ=255)                                                                                                gATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAAc                                <  2:1159157/140‑1 (MQ=255)                                                                                                    aTCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGc                            <  1:1033985/140‑1 (MQ=255)                                                                                                     tCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCt                           <  2:1014739/140‑1 (MQ=255)                                                                                                     tCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCCTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTCCCCTTCATGGCGCGCTGCCGCCACTCAACCCGCt                           <  2:550936/140‑1 (MQ=255)                                                                                                      cGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTg                          <  1:90868/140‑1 (MQ=255)                                                                                                        gCGTCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAg                        >  1:383946/1‑140 (MQ=255)                                                                                                           tCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCt                     >  1:643019/1‑140 (MQ=255)                                                                                                           tCCTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCt                     >  1:1183809/1‑140 (MQ=255)                                                                                                             cTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCttt                   <  2:187092/140‑1 (MQ=255)                                                                                                             cTGCTTGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCttt                   <  1:952407/140‑1 (MQ=255)                                                                                                                  tGATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGtt              <  2:383946/140‑1 (MQ=255)                                                                                                                   gATGGCGCGGCGCTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTa             <  1:658614/140‑1 (MQ=255)                                                                                                                      ggtgcggcgcTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGa          >  1:1202458/4‑140 (MQ=255)                                                                                                                        cgcggcgcTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAAt        >  2:329176/1‑140 (MQ=255)                                                                                                                        cgcggcgcTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAAt        >  2:228295/1‑140 (MQ=255)                                                                                                                          cggcgcTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAATaa      >  1:217278/1‑140 (MQ=255)                                                                                                                          cggcgcTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAATaa      <  2:819076/140‑1 (MQ=255)                                                                                                                          cggcgcTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAATaa      <  1:329176/140‑1 (MQ=255)                                                                                                                              gcTGCCGGCGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAATAAGATc  <  1:974965/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    TTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAATAAGATC  >  NC_000913/1176366‑1176630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑