Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,297,536	G→A	P196S (CCG→TCG)	adhE ←	aldehyde‑alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,297,536	0	G	A	100.0%	316.3 / NA	90	P196S (CCG→TCG)	adhE	aldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/45);  total (45/45)

GAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATC  >  NC_000913/1297402‑1297671                                                                                                                                       |                                                                                                                                        gAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAaccac                                                                                                                                    >  1:213271/1‑140 (MQ=255)      ttttGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTc                                                                                                                               >  2:598366/1‑140 (MQ=255)      ttttGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTc                                                                                                                               >  2:621390/1‑140 (MQ=255)        ttGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTcgc                                                                                                                             <  2:789596/140‑1 (MQ=255)         tGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTcgcg                                                                                                                            >  1:224369/1‑140 (MQ=255)           gACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAg                                                                                                                          <  1:459108/140‑1 (MQ=255)             cATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGa                                                                                                                        <  2:224369/140‑1 (MQ=255)                cAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTcgcg                                                                                                                            <  1:679638/133‑1 (MQ=255)                cAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTcgcg                                                                                                                            >  2:679638/1‑133 (MQ=255)                cAGGACAGATGCAATTGCACGGTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCa                                                                                                                     >  2:50595/1‑140 (MQ=255)                  gTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAgg                                                                                                                   <  1:835929/140‑1 (MQ=255)                    aCAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGtt                                                                                                                 <  1:1022340/140‑1 (MQ=255)                        aTGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATg                                                                                                             >  1:223415/1‑140 (MQ=255)                          gCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGtt                                                                                                                 <  1:1165013/134‑1 (MQ=255)                          gCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGtt                                                                                                                 >  2:1165013/1‑134 (MQ=255)                          gCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTc                                                                                                           >  2:218536/1‑140 (MQ=255)                            aaCTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTg                                                                                                         <  1:233790/140‑1 (MQ=255)                             aCTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTgg                                                                                                        >  2:311941/1‑140 (MQ=255)                             aCTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTgg                                                                                                        >  2:908274/1‑140 (MQ=255)                             aCTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTgg                                                                                                        >  1:1154962/1‑140 (MQ=255)                             aCTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTgg                                                                                                        >  2:711160/1‑140 (MQ=255)                                gCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGggtg                                                                                                     <  1:913937/140‑1 (MQ=255)                                gCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGggtg                                                                                                     <  1:448209/140‑1 (MQ=255)                                 cACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATc                                                                                                                      <  1:211546/122‑1 (MQ=255)                                 cACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATc                                                                                                                      >  2:211546/1‑122 (MQ=255)                                   cGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGgtggtg                                                                                                  <  1:311941/140‑1 (MQ=255)                                        gATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGGTCAGGGTGATGTCTGGGGGGTGCATCa                                                                                             >  2:719614/1‑140 (MQ=255)                                        gATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCa                                                                                             <  1:151506/140‑1 (MQ=255)                                        gATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCa                                                                                             <  2:1112730/140‑1 (MQ=255)                                        gATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCa                                                                                             <  1:711160/140‑1 (MQ=255)                                        gATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCa                                                                                             <  1:218536/140‑1 (MQ=255)                                        gATATCAGCAGTGTCAACGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCGCCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCa                                                                                             <  2:181695/140‑1 (MQ=255)                                         atatCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAg                                                                                            >  1:511031/1‑140 (MQ=255)                                           atCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTg                                                                                          >  2:828863/1‑140 (MQ=255)                                              agcagTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGt                                                                                       >  1:1214712/1‑140 (MQ=255)                                                cagTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTa                                                                                     >  2:740033/1‑140 (MQ=255)                                                cagTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTa                                                                                     <  2:1215963/140‑1 (MQ=255)                                                cagTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTa                                                                                     >  2:569012/1‑140 (MQ=255)                                                cagTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTa                                                                                     <  2:1163527/140‑1 (MQ=255)                                                cagTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGG‑GGTGCATCAGTGCGTTAg                                                                                    >  1:712923/1‑140 (MQ=255)                                                 agTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCa                                                                                             <  1:518950/131‑1 (MQ=255)                                                 agTTTCATCGATAACAACTGGAGTGTTGCCCGCACCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCa                                                                                             >  2:518950/1‑131 (MQ=255)                                                  gTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGa                                                                                   <  1:598366/140‑1 (MQ=255)                                                  gTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGa                                                                                   <  1:908274/140‑1 (MQ=255)                                                       aTCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGtt                                                                              <  1:883570/140‑1 (MQ=255)                                                       aTCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGtt                                                                              <  1:761072/140‑1 (MQ=255)                                                         cGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGa                                                                                   <  2:239957/133‑1 (MQ=255)                                                         cGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGa                                                                                   >  1:239957/1‑133 (MQ=255)                                                          gATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCaa                                                                           <  2:1214712/140‑1 (MQ=255)                                                               caacTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGtt                                                                              >  1:470253/1‑132 (MQ=255)                                                               caacTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGtt                                                                              <  2:470253/132‑1 (MQ=255)                                                                aacTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAg                                                                     >  1:190277/1‑140 (MQ=255)                                                                 acTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAgg                                                                    <  2:511031/140‑1 (MQ=255)                                                                   tGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGG‑GGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTg                                                                 <  2:712923/140‑1 (MQ=255)                                                                     gAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTga                                                                <  1:828863/140‑1 (MQ=255)                                                                      aGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgat                                                               >  1:661999/1‑140 (MQ=255)                                                                      aGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgat                                                               <  2:213271/140‑1 (MQ=255)                                                                         gtTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgatcga                                                            <  2:190277/140‑1 (MQ=255)                                                                          ttGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAAc                                                                          <  2:385814/125‑1 (MQ=255)                                                                          ttGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAAc                                                                          >  1:385814/1‑125 (MQ=255)                                                                           tGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgatcgatc                                                          <  1:639076/140‑1 (MQ=255)                                                                              ccGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAg                                                       <  2:1096211/140‑1 (MQ=255)                                                                               cgcgCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAGCCAGGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGTTTGATCGATCCAGc                                                      <  2:661999/140‑1 (MQ=255)                                                                                  gcCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGa                                                   <  1:719614/140‑1 (MQ=255)                                                                                       cacCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGa                                              >  2:368955/1‑140 (MQ=255)                                                                                       cacCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGa                                              >  2:424447/1‑140 (MQ=255)                                                                                          cGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCt                                           <  1:621390/140‑1 (MQ=255)                                                                                            aTAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCttt                                         >  2:572958/1‑140 (MQ=255)                                                                                            aTAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCttt                                         >  1:188459/1‑140 (MQ=255)                                                                                            aTAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCttt                                         >  1:1040784/1‑140 (MQ=255)                                                                                            aTAGCTGGTGTACCGGAGCTGTGTGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCAGCAGTGCGTTAGACAGTTCAACAGAAGGCTGATCGATCCAGCCGATCAGATCttt                                         >  2:1210482/1‑140 (MQ=255)                                                                                                 tGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGa                                              <  1:1012928/130‑1 (MQ=255)                                                                                                 tGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGa                                              >  2:1012928/1‑130 (MQ=255)                                                                                                 tGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAg                                    >  1:1123492/1‑140 (MQ=255)                                                                                                   gTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCa                                  >  2:1015059/1‑140 (MQ=255)                                                                                                              cTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGAt                       >  1:585806/1‑140 (MQ=255)                                                                                                              cTGTATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGAt                       >  1:154691/1‑140 (MQ=255)                                                                                                                 tATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATagc                    >  2:304303/1‑140 (MQ=255)                                                                                                                 tATGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATagc                    >  1:831179/1‑140 (MQ=255)                                                                                                                  aTGCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATagca                   <  1:740033/140‑1 (MQ=255)                                                                                                                    gCGGCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATagcagc                 <  1:1021912/140‑1 (MQ=255)                                                                                                                       gCTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTg              <  1:615847/140‑1 (MQ=255)                                                                                                                        cTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTg              <  2:11834/139‑1 (MQ=255)                                                                                                                        cTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTg              >  1:11834/1‑139 (MQ=255)                                                                                                                        cTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGc             <  1:569012/140‑1 (MQ=255)                                                                                                                        cTTTAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGc             <  1:424447/140‑1 (MQ=255)                                                                                                                           tAACCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGa          >  2:88557/1‑140 (MQ=255)                                                                                                                             aCCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAAc        >  2:503228/1‑140 (MQ=255)                                                                                                                             aCCATGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAAc        >  2:870708/1‑140 (MQ=255)                                                                                                                                 tGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATagca                   <  2:1198173/125‑1 (MQ=255)                                                                                                                                 tGCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATagca                   >  1:1198173/1‑125 (MQ=255)                                                                                                                                  gCCCGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGatat   >  2:379440/1‑140 (MQ=255)                                                                                                                                   cccGAACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATc  <  1:304303/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGGCAGCGATAGCAGCCTGCAGAACGATATC  >  NC_000913/1297402‑1297671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑