Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	68,258	A→G	V27A (GTC→GCC)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	68,258	0	A	G	100.0%	190.4 / NA	54	V27A (GTC→GCC)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/29);  total (25/29)

CGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAG  >  NC_000913/68123‑68391                                                                                                                                        |                                                                                                                                      cGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTg                                                                                                                                   >  1:733599/1‑140 (MQ=255) cGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTg                                                                                                                                   >  1:460114/1‑140 (MQ=255)      gtcgtAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCa                                                                                                                              >  1:753900/1‑140 (MQ=255)         gtAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAggg                                                                                                                           >  2:313267/1‑140 (MQ=255)            aTTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGttt                                                                                                                        <  2:794733/140‑1 (MQ=255)            aTTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGttt                                                                                                                        <  2:1144739/140‑1 (MQ=255)            aTTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGttt                                                                                                                        <  1:517295/140‑1 (MQ=255)             ttCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTc                                                                                                                       >  1:453466/1‑140 (MQ=255)              tcgcgtcgcgGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTcc                                                                                                                      <  2:429781/140‑1 (MQ=255)              tcgcgtcgcgGCAAATAGCGGTGATTTCATCCGGCGGGGTGCCCAGCGGTTTCAAAACCAGATTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTcc                                                                                                                      >  1:78585/1‑140 (MQ=255)                 cgtcgcgGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCggg                                                                                                                   <  2:366180/140‑1 (MQ=255)                  gtcgcgGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGc                                                                                                                  >  2:570540/1‑140 (MQ=255)                              aGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATgct                                                                                                      >  2:511057/1‑140 (MQ=255)                                cGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATgctgg                                                                                                    <  1:364590/140‑1 (MQ=255)                                   tGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATgctggctg                                                                                                 >  2:475377/1‑140 (MQ=255)                                    gATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGc                                                                                                <  1:98389/140‑1 (MQ=255)                                     aTTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGcc                                                                                               >  2:618108/1‑140 (MQ=255)                                          aTCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGa                                                                                          >  2:1185414/1‑140 (MQ=255)                                               gCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAc                                                                                     >  1:720799/1‑140 (MQ=255)                                                 gtggtgCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACca                                                                                   <  1:407046/140‑1 (MQ=255)                                                 gtggtgCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACca                                                                                   <  1:313267/140‑1 (MQ=255)                                                 gtggtgCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACca                                                                                   <  1:86754/140‑1 (MQ=255)                                                 gtggtgCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAAGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACca                                                                                   <  1:112732/140‑1 (MQ=255)                                                   ggtgCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACcaca                                                                                 <  2:1112152/140‑1 (MQ=255)                                                         cAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCAt                                                                           >  2:700927/1‑140 (MQ=255)                                                               tttCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAcc                                                                                    >  1:477714/1‑125 (MQ=255)                                                               tttCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAAcc                                                                                    <  2:477714/125‑1 (MQ=255)                                                                      acCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAAt                                                              >  1:1059587/1‑140 (MQ=255)                                                                        cAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCaaaa                                                                >  2:947785/1‑136 (MQ=255)                                                                        cAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCaaaa                                                                <  1:947785/136‑1 (MQ=255)                                                                        cAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCg                                                            <  1:515813/140‑1 (MQ=255)                                                                               cAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTAt                                                     <  2:685002/140‑1 (MQ=255)                                                                                aGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATc                                                    <  2:1059587/140‑1 (MQ=255)                                                                                 gggCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCg                                                   <  2:453466/140‑1 (MQ=255)                                                                                 gggCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCg                                                   <  1:511057/140‑1 (MQ=255)                                                                                    cAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCgtgt                                                >  1:836773/1‑140 (MQ=255)                                                                                    cAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCgtgt                                                <  1:450042/140‑1 (MQ=255)                                                                                                   aTTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCa                                 <  2:41633/140‑1 (MQ=255)                                                                                                   aTTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCa                                 <  2:836773/140‑1 (MQ=255)                                                                                                         cgcATTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGcc                           >  1:238821/1‑140 (MQ=255)                                                                                                            aTTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTgg                        >  2:1210883/1‑140 (MQ=255)                                                                                                            aTTAACGACGTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTgg                        >  2:636170/1‑140 (MQ=255)                                                                                                                 cgacgTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGc                   <  2:238821/140‑1 (MQ=255)                                                                                                                     gTGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCtgt               >  1:854488/1‑140 (MQ=255)                                                                                                                      tGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACgg                             >  1:655258/1‑125 (MQ=255)                                                                                                                      tGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACgg                             <  2:655258/125‑1 (MQ=255)                                                                                                                      tGCTCGGCATGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCtgtg              >  2:586588/1‑140 (MQ=255)                                                                                                                               tGTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCgg     <  1:475377/140‑1 (MQ=255)                                                                                                                                gTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGa    >  2:560321/1‑140 (MQ=255)                                                                                                                                gTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGa    <  1:1185414/140‑1 (MQ=255)                                                                                                                                gTTGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGa    <  1:1109262/140‑1 (MQ=255)                                                                                                                                  tGGGTGGCCTGACGCAGGGTTTCCGGGCCATGCAGATGCTGGCTGCTAATGACAAACCGCACTCCATAAAAATCAAAAAACGTCATTATCGTGTCCTTATAGATTCGCAACGGCGTGGGCAGCCTGTGCCGGGGCGGAAg  <  2:78585/140‑1 (MQ=255)                                                                                                                                  tGGGTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAg  <  2:865456/140‑1 (MQ=255)                                                                                                                                     gTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGa    <  2:82562/135‑1 (MQ=255)                                                                                                                                     gTGGCCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGa    >  1:82562/1‑135 (MQ=255)                                                                                                                                        |                                                                                                                                      CGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAG  >  NC_000913/68123‑68391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑