| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,390,048 | A→G | V184A (GTA→GCA) | mpaA ← | murein tripeptide amidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,390,048 | 0 | A | G | 96.4% | 178.9 / ‑2.0 | 55 | V184A (GTA→GCA) | mpaA | murein tripeptide amidase A |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (19/34); total (20/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
TGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCT > NC_000913/1389915‑1390183 | tGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCagag > 1:241380/1‑140 (MQ=255) gCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAgg < 2:780763/140‑1 (MQ=255) ccATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGc > 1:190307/1‑140 (MQ=255) cATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCa < 2:651667/140‑1 (MQ=255) cGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAAtt > 2:505349/1‑140 (MQ=255) cAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCa > 2:787235/1‑140 (MQ=255) aaaGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCaa < 2:547863/140‑1 (MQ=255) gaTATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGcc < 2:780960/140‑1 (MQ=255) tatTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTg > 1:1139819/1‑140 (MQ=255) cGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGagccagc > 2:1095611/1‑140 (MQ=255) cTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAgccagcca < 2:808360/140‑1 (MQ=255) gCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTc > 1:1133458/1‑140 (MQ=255) gCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTc < 1:505349/140‑1 (MQ=255) gaggagATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTc < 2:1139819/140‑1 (MQ=255) aggagATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCg > 1:28702/1‑140 (MQ=255) aggagATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCg < 1:367557/140‑1 (MQ=255) ggagATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGc < 2:578905/140‑1 (MQ=255) ggagATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGc < 2:531092/140‑1 (MQ=255) gagaTGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCt < 2:241380/140‑1 (MQ=255) gagaTGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCt < 1:1005025/140‑1 (MQ=255) aTGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCtgtg > 1:1044315/1‑140 (MQ=255) gggCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCtg > 2:1159068/1‑136 (MQ=255) gggCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCtg < 1:1159068/136‑1 (MQ=255) gAAACTCCGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGa > 2:786942/1‑140 (MQ=255) ctctGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCtt < 2:1061014/140‑1 (MQ=255) cGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATa < 2:28702/140‑1 (MQ=255) gTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACa < 2:1133458/140‑1 (MQ=255) tGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAg < 1:787235/140‑1 (MQ=255) atgcaatgcaAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGc < 1:206126/140‑1 (MQ=255) aatgcaaGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGt < 1:360263/140‑1 (MQ=255) aatgcaaGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGt < 2:70660/140‑1 (MQ=255) gTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGt > 2:864529/1‑140 (MQ=255) cAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGa < 2:190307/140‑1 (MQ=255) tCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATga > 2:935861/1‑140 (MQ=255) cTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATgag < 2:1044315/140‑1 (MQ=255) tGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCATTACAGGCCAGTGGATCGTGGAATgaga < 1:325920/140‑1 (MQ=255) gCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGac < 1:931850/140‑1 (MQ=255) gCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGac < 2:618215/140‑1 (MQ=255) cAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATgaga < 1:316264/133‑1 (MQ=255) cAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATgaga > 2:316264/1‑133 (MQ=255) aCTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTTGATCGTGGAATGAGACCACCcagg > 2:562530/1‑140 (MQ=255) aCTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCcagg > 2:24437/1‑140 (MQ=255) gCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGaa < 2:816331/123‑1 (MQ=255) gCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGaa > 1:816331/1‑123 (MQ=255) cGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGt < 1:74943/140‑1 (MQ=255) agagCCAGGCGTTTCATAAACGACGCTCGTTGCCAGAGGCAATTCGAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGaa < 2:717844/140‑1 (MQ=255) gCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGc < 1:1095611/140‑1 (MQ=255) gCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGc > 2:758684/1‑140 (MQ=255) gCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGc < 1:766451/140‑1 (MQ=255) gCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGc < 1:24437/140‑1 (MQ=255) gCCAGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGc < 1:1105408/140‑1 (MQ=255) aGGCGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGgt > 1:955298/1‑140 (MQ=255) cGTTTCATAACCGACGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGta < 1:786942/140‑1 (MQ=255) aCGCTGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAg > 1:519989/1‑140 (MQ=255) tGGTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGcc < 1:935861/140‑1 (MQ=255) ggTTGCCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCt < 1:758684/140‑1 (MQ=255) | TGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGCCAGGCGTTTCATAACCGACGCTGGTTACCAGAGGCAATTCAAACGCCTGAGCCAGCCATTCGCCTAATTCGCTGTGTCTGGGATCTTCAATACAGGCCAGTGGATCGTGGAATGAGACCACCCAGGCAGGTTGAATGCGGTGTATAAGCTGGCACAGCGCCT > NC_000913/1389915‑1390183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |