| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,433,698 | T→C | intergenic (‑24/+293) | pinR ← / ← ynaE | Rac prophage; putative site‑specific recombinase/Rac prophage; uncharacterized protein YnaE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,433,698 | 0 | T | C | 98.3% | 128.7 / ‑4.3 | 59 | intergenic (‑24/+293) | pinR/ynaE | Rac prophage; putative site‑specific recombinase/Rac prophage; uncharacterized protein YnaE |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (20/38); total (20/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01 | |||||||||||
TTCGATTATTTGCTGAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTT > NC_000913/1433561‑1433801 | ttcGATTATTTGCTGAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGgcgt > 2:91947/1‑140 (MQ=17) tcGATTATTTGCTGAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGtt < 2:534742/140‑1 (MQ=17) gATTATTTGCTGAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTtca < 2:489089/140‑1 (MQ=18) gATTATTTGCTGAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTtca < 1:1163296/140‑1 (MQ=18) gATTATTTGCTGAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTtca < 1:96120/140‑1 (MQ=17) gATTATTTGCTGAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTtca < 2:130071/140‑1 (MQ=18) ttattTGCTGAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTtcata > 1:213223/1‑140 (MQ=17) tttGCTGAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTtcatatca < 1:1154854/140‑1 (MQ=18) ttGCTGAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTtcatatcat > 1:526574/1‑140 (MQ=17) tGCTGAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTtcatatcata < 1:18260/140‑1 (MQ=18) gAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGc > 1:1134070/1‑140 (MQ=17) aGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCa > 2:1093162/1‑140 (MQ=17) aGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCa > 2:222875/1‑140 (MQ=17) aGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCa > 2:714754/1‑140 (MQ=17) tttGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCAtct < 1:455524/140‑1 (MQ=17) ttGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCAtctc > 1:1018635/1‑140 (MQ=17) tGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCa > 2:892475/1‑135 (MQ=17) tGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCa < 1:892475/135‑1 (MQ=18) tGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCa < 2:1005277/140‑1 (MQ=18) ttttAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGaa < 1:410778/140‑1 (MQ=18) aaaaCCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAaata > 1:458712/1‑140 (MQ=17) aaaaCCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAaata > 2:320342/1‑140 (MQ=17) cTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTAt < 2:1114883/140‑1 (MQ=18) cTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTAt < 2:526574/140‑1 (MQ=18) tGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATg > 2:874850/1‑140 (MQ=17) cACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGat < 1:714754/140‑1 (MQ=17) cACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGat < 1:1093162/140‑1 (MQ=17) tttCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAATAAGCAAAAATTCGAAACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCAACTCAGAAAATAATTATGATTATTTTtgt < 2:480353/140‑1 (MQ=255) ttCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTtgtg < 1:874850/140‑1 (MQ=17) ttCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTtgtg < 2:1001615/140‑1 (MQ=17) ttCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTtgtg < 2:846265/140‑1 (MQ=17) tCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGc < 2:566685/140‑1 (MQ=17) cccGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCa < 2:302277/140‑1 (MQ=17) ccGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCat < 1:91947/140‑1 (MQ=17) cGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCata < 2:985667/140‑1 (MQ=17) cGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCata < 1:681238/140‑1 (MQ=17) cGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCata < 1:324775/140‑1 (MQ=17) ggCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCatat < 1:325729/140‑1 (MQ=17) ggCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCatat < 2:1134070/140‑1 (MQ=17) ggCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCatat > 2:325729/1‑140 (MQ=17) gCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATAtt < 2:1019152/140‑1 (MQ=17) cGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATAttt < 2:156011/140‑1 (MQ=32) tggtCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGtt < 2:1018635/140‑1 (MQ=17) aTCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAAt > 1:864466/1‑140 (MQ=18) gCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACg < 2:1163225/140‑1 (MQ=17) cGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGa > 1:136216/1‑140 (MQ=17) cGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGa > 2:190214/1‑140 (MQ=18) ttGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAAt < 2:265204/140‑1 (MQ=17) ttGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAAt < 2:213223/140‑1 (MQ=17) gATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGc < 1:190214/140‑1 (MQ=17) gATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGc > 1:991854/1‑140 (MQ=18) gATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGc > 2:1028546/1‑140 (MQ=18) gATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGc > 1:47709/1‑140 (MQ=18) atatCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAAt > 1:1005307/1‑137 (MQ=18) atatCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAAt < 2:1005307/137‑1 (MQ=17) gACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTATATGTACACGTTCGAAAATAAACGAATGCGTATGCa < 2:458712/140‑1 (MQ=17) aCAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGCGTATGCaa < 2:45571/140‑1 (MQ=17) aaGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCg < 1:913491/140‑1 (MQ=17) aaGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCg < 2:261443/140‑1 (MQ=17) aaaTTCGAGACATAGTGAGACTCTATACGAAATTGGCGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAAttt > 1:648180/1‑140 (MQ=17) | TTCGATTATTTGCTGAGGTTTGATTTTAAAACCTGCACTTTCGATTTCCCGGCGTTGATTTTCGGTGGTCTGATCCAGCGTTGATATCCGACAGTAAGCAAAAATTCGAGACATAGTGAGACTCTATACGAAATTGGTGTTCATATCATAATGCATCTCAGAAAATAATTATGATTATTTTTGTGCATATTTGTATGTACACGTTCGAAAATAAACGAATGCGTATGCAACCCCGTAATTT > NC_000913/1433561‑1433801 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |