Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,494,533	A→G	V180A (GTT→GCT)	ydcI ←	putative DNA‑binding transcriptional repressor YdcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,494,533	0	A	G	100.0%	225.6 / NA	63	V180A (GTT→GCT)	ydcI	putative DNA‑binding transcriptional repressor YdcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/22);  total (41/22)

GCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCA  >  NC_000913/1494403‑1494662                                                                                                                                   |                                                                                                                                  gCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCa                                                                                                                          >  2:838048/1‑140 (MQ=255)  cccTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCaa                                                                                                                         >  1:651842/1‑140 (MQ=255)  cccTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCaa                                                                                                                         <  1:1107944/140‑1 (MQ=255)  cccTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCaa                                                                                                                         >  2:1160109/1‑140 (MQ=255)  cccTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCaa                                                                                                                         >  1:1141841/1‑140 (MQ=255)  cccTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCaa                                                                                                                         <  2:79626/140‑1 (MQ=255)            gCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGa                                                                                                               <  1:1160109/140‑1 (MQ=255)            gCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGa                                                                                                               >  1:1212003/1‑140 (MQ=255)                   tGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAAcagca                                                                                                        >  1:958416/1‑140 (MQ=255)                   tGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAAcagca                                                                                                        <  2:651842/140‑1 (MQ=255)                   tGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAAcag                                                                                                          <  1:54110/138‑1 (MQ=255)                   tGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAAcag                                                                                                          >  2:54110/1‑138 (MQ=255)                       tCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAAcag                                                                                                          >  2:755846/1‑134 (MQ=255)                       tCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAAcag                                                                                                          <  1:755846/134‑1 (MQ=255)                       tCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTc                                                                                                    >  2:737373/1‑140 (MQ=255)                       tCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTc                                                                                                    >  2:233768/1‑140 (MQ=255)                       tCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTc                                                                                                    >  2:922897/1‑140 (MQ=255)                            aTGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTc                                                                                                    <  2:555199/135‑1 (MQ=255)                            aTGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTc                                                                                                    >  1:555199/1‑135 (MQ=255)                            aTGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAAt                                                                                               >  1:1134197/1‑140 (MQ=255)                             tGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAAtt                                                                                              <  1:737373/140‑1 (MQ=255)                             tGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAAtt                                                                                              <  2:1212003/140‑1 (MQ=255)                             tGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAAtt                                                                                              >  2:960005/1‑140 (MQ=255)                                tggcgtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAg                                                                                           >  1:988096/1‑140 (MQ=255)                                 ggcgtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAAcagca                                                                                                        >  2:351176/1‑126 (MQ=255)                                 ggcgtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAAcagca                                                                                                        <  1:351176/126‑1 (MQ=255)                                  gcgtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGcc                                                                                         >  2:519874/1‑140 (MQ=255)                                  gcgtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGcc                                                                                         <  1:960005/140‑1 (MQ=255)                                  gcgtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGcc                                                                                         >  2:1153706/1‑140 (MQ=255)                                  gcgtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGcc                                                                                         <  2:1141841/140‑1 (MQ=255)                                       gcgcAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGtc                                                                                    >  1:431490/1‑140 (MQ=255)                                         gcAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGtcat                                                                                  <  2:613856/140‑1 (MQ=255)                                            gTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAg                                                                               >  2:535382/1‑140 (MQ=255)                                            gTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAg                                                                               <  1:41184/140‑1 (MQ=255)                                              gCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGtt                                                                             <  1:17765/140‑1 (MQ=255)                                              gCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGtt                                                                             >  1:297373/1‑140 (MQ=255)                                                  tCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAg                                                                          <  1:1025865/139‑1 (MQ=255)                                                  tCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAg                                                                          >  2:1025865/1‑139 (MQ=255)                                                      gTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCt                                                                     <  1:922897/140‑1 (MQ=255)                                                           aCAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACAt                                                                <  1:749403/140‑1 (MQ=255)                                                             aaCGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATcc                                                              >  2:810506/1‑140 (MQ=255)                                                                gACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGAc                                                           >  2:559594/1‑140 (MQ=255)                                                                 aCCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGAcc                                                          >  2:261228/1‑140 (MQ=255)                                                                 aCCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGAcc                                                          >  1:751067/1‑140 (MQ=255)                                                                    ggCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAAt                                                       >  1:494990/1‑140 (MQ=255)                                                                     gCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGTGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATg                                                      >  2:427706/1‑140 (MQ=255)                                                                               cACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGa                                            >  1:129675/1‑140 (MQ=255)                                                                                    ggCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTcc                                       >  2:1147678/1‑140 (MQ=255)                                                                                    ggCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTcc                                       >  1:993742/1‑140 (MQ=255)                                                                                    ggCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTcc                                       >  1:1005935/1‑140 (MQ=255)                                                                                    ggCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTcc                                       >  1:726654/1‑140 (MQ=255)                                                                                    ggCTTAGCGTTACGTTCTCCTGAAGTAGCGGGAGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTcc                                       >  1:660246/1‑140 (MQ=255)                                                                                     gCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTccc                                      <  1:423225/140‑1 (MQ=255)                                                                                                cGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTaaa                           >  2:828711/1‑140 (MQ=255)                                                                                                           aGTAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAAt                <  2:958416/140‑1 (MQ=255)                                                                                                             tAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCa              >  1:98016/1‑140 (MQ=255)                                                                                                             tAGCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCa              <  2:431490/140‑1 (MQ=255)                                                                                                               gCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATa            >  1:195191/1‑140 (MQ=255)                                                                                                               gCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATa            >  2:758213/1‑140 (MQ=255)                                                                                                               gCGGGTGATTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATa            >  1:633280/1‑140 (MQ=255)                                                                                                                  ggTGATTAGGGGGGACAGCCCGCTCAAACGATTCAAGAAACAGCATTTCGTAATTAGGCCGGGTCACCAGTTCAGGATCTGACACCCGACCAATCCCGATATCAATTTCCCCGGTTTTAAAGCCCGCCAGAATCATAggg         <  2:195191/140‑1 (MQ=255)                                                                                                                      aTTAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTAc     >  1:151164/1‑140 (MQ=255)                                                                                                                       ttAGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACt    <  1:838048/140‑1 (MQ=255)                                                                                                                       ttAGGGCGGACAGCAAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACt    >  2:142190/1‑140 (MQ=255)                                                                                                                         aGGGCGGACAGCCAGCTTCAACGATTCAAGAAACAGCATTTCTTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGCCCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCa  <  2:177181/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                  GCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAACCCGCCAGAATCATAGGGTTACTCA  >  NC_000913/1494403‑1494662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑