Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,512,747	T→C	V365A (GTT→GCT)	ydcS →	putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,512,747	0	T	C	100.0%	215.5 / NA	61	V365A (GTT→GCT)	ydcS	putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/32);  total (29/32)

GTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTGCGCGCAGTAGATGG  >  NC_000913/1512616‑1512881                                                                                                                                    |                                                                                                                                       gTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACa                                                                                                                                <  2:28485/140‑1 (MQ=255)   tACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGt                                                                                                                              >  2:864299/1‑140 (MQ=255)     ccGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTCGCTACCTACAGTCg                                                                                                                            <  2:902403/140‑1 (MQ=255)       ggTAGTGCCGGAAGGGTGTAAAGCCAGTACGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCt                                                                                                                          <  2:447932/140‑1 (MQ=255)        gTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTg                                                                                                                         >  1:400087/1‑140 (MQ=255)         tAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTgg                                                                                                                        >  2:702182/1‑140 (MQ=255)            tGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACg                                                                                                                     <  1:1133316/140‑1 (MQ=255)            tGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACg                                                                                                                     >  2:135556/1‑140 (MQ=255)            tGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACg                                                                                                                     <  1:780200/140‑1 (MQ=255)              ccGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCa                                                                                                                   >  2:411576/1‑140 (MQ=255)                 gAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGa                                                                                                                >  1:244167/1‑140 (MQ=255)                  aaGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGAt                                                                                                               <  1:123608/140‑1 (MQ=255)                   aGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGAc                                                                                                                      >  2:330395/1‑132 (MQ=255)                   aGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGAc                                                                                                                      <  1:330395/132‑1 (MQ=255)                        gtAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACAtt                                                                                                         <  2:262936/140‑1 (MQ=255)                                aGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTAt                                                                                                 >  1:577491/1‑140 (MQ=255)                                 gTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATg                                                                                                >  2:390377/1‑140 (MQ=255)                                 gTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATg                                                                                                >  1:366664/1‑140 (MQ=255)                                 gTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATg                                                                                                >  1:145999/1‑140 (MQ=255)                                   ccGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATggg                                                                                              <  1:135556/140‑1 (MQ=255)                                   ccGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATggg                                                                                              <  1:1166956/140‑1 (MQ=255)                                          taGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGc                                                                                       >  2:562623/1‑140 (MQ=255)                                            ggCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTa                                                                                     >  1:503602/1‑140 (MQ=255)                                             gCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTaa                                                                                    >  1:129677/1‑140 (MQ=255)                                                   aaGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACACAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGc                                                                              >  1:1031814/1‑140 (MQ=255)                                                       ttGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTggg                                                                          <  2:985855/140‑1 (MQ=255)                                                       ttGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTggg                                                                          >  1:371258/1‑140 (MQ=255)                                                              aCCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCttt                                                                   <  1:562623/140‑1 (MQ=255)                                                              aCCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCttt                                                                   <  2:503602/140‑1 (MQ=255)                                                                 aaCGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTggg                                                                          <  2:375962/130‑1 (MQ=255)                                                                 aaCGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTggg                                                                          >  1:375962/1‑130 (MQ=255)                                                                    ggTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACg                                                             >  2:996000/1‑140 (MQ=255)                                                                      ttttAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTa                                                           <  1:1162949/140‑1 (MQ=255)                                                                        ttAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACg                                                         <  1:198495/140‑1 (MQ=255)                                                                           aCTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAg                                                      <  2:145999/140‑1 (MQ=255)                                                                           aCTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAg                                                      <  2:762730/140‑1 (MQ=255)                                                                           aCTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAg                                                      <  2:366664/140‑1 (MQ=255)                                                                             tATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTg                                                    <  2:129677/140‑1 (MQ=255)                                                                             tATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTg                                                    <  2:337510/140‑1 (MQ=255)                                                                                ttCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAg                                                      <  1:713950/135‑1 (MQ=255)                                                                                ttCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAg                                                      >  2:713950/1‑135 (MQ=255)                                                                                ttCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAg                                                 >  1:1190110/1‑140 (MQ=255)                                                                                ttCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAg                                                 >  1:807743/1‑140 (MQ=255)                                                                                ttCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAg                                                 >  1:624693/1‑140 (MQ=255)                                                                                    aCAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTa                                                           >  1:44668/1‑126 (MQ=255)                                                                                    aCAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTa                                                           <  2:44668/126‑1 (MQ=255)                                                                                                  tGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCgg                               <  2:400087/140‑1 (MQ=255)                                                                                                  tGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGCCTCGCgg                               <  1:520371/140‑1 (MQ=255)                                                                                                              ataGCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACACAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGAc                   <  2:1031814/140‑1 (MQ=255)                                                                                                                 gCAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTg                <  1:199127/140‑1 (MQ=255)                                                                                                                  cAGAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTgc               <  1:411576/140‑1 (MQ=255)                                                                                                                    gAAGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTgcgc             >  2:164485/1‑140 (MQ=255)                                                                                                                     aaGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTgcgcg            <  1:495463/140‑1 (MQ=255)                                                                                                                     aaGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTgcgcg            <  2:807743/140‑1 (MQ=255)                                                                                                                      aGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTgcgcgc           >  1:539912/1‑140 (MQ=255)                                                                                                                      aGGGGGCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTgcgcgc           <  2:244167/140‑1 (MQ=255)                                                                                                                       gggggCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTGCGCGCa          >  2:696070/1‑140 (MQ=255)                                                                                                                          ggCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTGCGCGCAGTa       >  1:26870/1‑140 (MQ=255)                                                                                                                          ggCAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTGCGCGCAGTa       >  1:960120/1‑140 (MQ=255)                                                                                                                            cAAGTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTGCGCGCAGTAGa     >  1:1183504/1‑140 (MQ=255)                                                                                                                               gTTTGCTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTGCGCGCAGTAGATgg  <  2:1190110/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       GTTACCGGTAGTGCCGGAAGGGTGTAAAGCCAGTCCGTTATTAGGCGAAAAAGGTTGTGAAACCAACGGTTTTAACTATTTCGACAAAATCGCCTTCTGGAAAACGCCTATAGCAGAAGGGGGCAAGTTTGTTCCCTACAGTCGCTGGACGCAGGATTACATTGCCATTATGGGCGGTCGCTAACTTCGCTGGGGTGCTTTATGACGTACGCAGTGGAGTTTGACAACGTCTCGCGGTTGTACGGTGACGTGCGCGCAGTAGATGG  >  NC_000913/1512616‑1512881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑