| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,570,471 | T→C | T7A (ACA→GCA) | gadC ← | L‑glutamate:4‑aminobutyrate antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,570,471 | 0 | T | C | 97.9% | 163.9 / ‑3.3 | 47 | coding (19/1536 nt) | gadC | L‑glutamate:4‑aminobutyrate antiporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (25/21); minor base . (0/1); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
TAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAA > NC_000913/1570334‑1570607 | tAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACctgcct > 1:782888/1‑140 (MQ=255)tAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACctgcct > 2:524150/1‑140 (MQ=255) cccGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTg < 1:1215590/140‑1 (MQ=255) gCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCAAAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATg < 2:1185320/140‑1 (MQ=255) cTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTa < 2:1080333/140‑1 (MQ=255) tAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAg < 1:931905/140‑1 (MQ=255) aagaCTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCAtattat > 2:300236/1‑136 (MQ=255) aagaCTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCAtattat < 1:300236/136‑1 (MQ=255) gaCTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGGCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCt > 2:1083787/1‑140 (MQ=255) aCTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTa > 1:829425/1‑140 (MQ=255) aaTGAAAAGCCCGATGTTGCGAAGGTAGGGTTTTCAAAAACAGCCATTCCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTCCCTGCCTGTACTGATGTAGCCATATTATCCCCCTaaaa < 2:782888/140‑1 (MQ=255) aTGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGcc < 1:459406/122‑1 (MQ=255) aTGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGcc > 2:459406/1‑122 (MQ=255) gAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACgg < 1:1118102/140‑1 (MQ=255) cccGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTc < 1:651522/139‑1 (MQ=255) cccGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTc > 2:651522/1‑139 (MQ=255) cGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTg > 2:1146216/1‑140 (MQ=255) cGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAAcc > 2:1046549/1‑140 (MQ=255) cGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTG‑CTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCg < 1:663010/140‑1 (MQ=255) gTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAAc < 1:30830/134‑1 (MQ=255) gTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAAc > 2:30830/1‑134 (MQ=255) gTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCAcc > 1:802437/1‑140 (MQ=255) gTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCAcc > 2:684833/1‑140 (MQ=255) gTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCAcc > 1:279211/1‑140 (MQ=255) ggTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGtt < 1:866120/140‑1 (MQ=255) ggTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGtt < 2:829425/140‑1 (MQ=255) tCATAAACAGCCATCACCATCGATTCCGTTAGGCCAAAGAATCCAGGTAATGTGAGATCTTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGtt < 2:802437/140‑1 (MQ=255) cATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCGAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTc > 2:1082580/1‑140 (MQ=255) cATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTc > 1:481203/1‑140 (MQ=255) cATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTc > 2:99830/1‑140 (MQ=255) aTAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCt > 1:881733/1‑140 (MQ=255) aaaCAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGa > 1:1124416/1‑140 (MQ=255) cAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGc < 2:30827/140‑1 (MQ=255) ccATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa > 2:768111/1‑140 (MQ=255) ccATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa > 1:447730/1‑140 (MQ=255) ccATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa > 2:998396/1‑140 (MQ=255) aTTACCATCGATGCCGTTACGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTAtt > 2:193194/1‑140 (MQ=255) ccATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGa < 1:998396/140‑1 (MQ=255) cGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACaa > 2:709202/1‑140 (MQ=255) aTGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAata < 1:1046549/140‑1 (MQ=255) gCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAatat < 1:822117/139‑1 (MQ=255) gCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAatat > 2:822117/1‑139 (MQ=255) ccGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATAttt > 1:782570/1‑140 (MQ=255) ttATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATAtttccg < 2:881733/140‑4 (MQ=255) tATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCg < 1:99830/140‑1 (MQ=255) gCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGc < 2:279211/140‑1 (MQ=255) tCCAAGTAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCa > 1:1108567/1‑140 (MQ=255) tAATGTGAGCTGCTTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAAc < 1:586504/140‑1 (MQ=255) ctgctTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTgg > 1:220437/1‑140 (MQ=255) tgctTAGCTTTACCTGCCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGa < 1:1146216/140‑1 (MQ=255) ctgcctgtACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGaaa < 2:481203/140‑1 (MQ=255) | TAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATTCAGAACAATATTTTCCGTTGCTAATGCCAGTGAACAGACTTTGGAAATTGTCCCGAAA > NC_000913/1570334‑1570607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |