Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,884	A→C	R55R (CGT→CGG)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,884	0	A	C	100.0%	198.5 / NA	57	R55R (CGT→CGG)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/23);  total (34/23)

TCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTG  >  NC_000913/69756‑70019                                                                                                                                 |                                                                                                                                        tCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGatgatg                                                                                                                              >  2:191416/1‑140 (MQ=255) tCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGatgatg                                                                                                                              >  2:353102/1‑140 (MQ=255)     cATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACgg                                                                                                                          <  2:1119278/140‑1 (MQ=255)      aTCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGa                                                                                                                         >  2:444958/1‑140 (MQ=255)       tCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGaa                                                                                                                        <  1:191416/140‑1 (MQ=255)               tGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTAt                                                                                                                >  1:217395/1‑140 (MQ=255)                gCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTAtt                                                                                                               <  1:1128266/140‑1 (MQ=255)                     cGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCgggg                                                                                                          <  2:217395/140‑1 (MQ=255)                     cGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCgggg                                                                                                          >  1:585650/1‑140 (MQ=255)                       tCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCa                                                                                                        >  1:243686/1‑140 (MQ=255)                         gAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATc                                                                                                      >  1:498356/1‑140 (MQ=255)                         gAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATc                                                                                                      >  1:808474/1‑140 (MQ=255)                         gAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATc                                                                                                      >  2:393725/1‑140 (MQ=255)                              ggTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACaaa                                                                                                 >  1:955140/1‑140 (MQ=255)                                  cTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTg                                                                                             <  1:393725/140‑1 (MQ=255)                                  cTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTg                                                                                             >  1:72219/1‑140 (MQ=255)                                     tCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGccc                                                                                          >  1:647774/1‑140 (MQ=255)                                     tCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGccc                                                                                          >  1:687692/1‑140 (MQ=255)                                     tCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGccc                                                                                          >  1:1016541/1‑140 (MQ=255)                                     tCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCGCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGccc                                                                                          <  1:444958/140‑1 (MQ=255)                                        aCGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCttt                                                                                       >  1:939738/1‑140 (MQ=255)                                             aaTCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGcc                                                                                  <  1:250425/140‑1 (MQ=255)                                             aaTCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGcc                                                                                  <  1:353102/140‑1 (MQ=255)                                                    aCCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACgggg                                                                           >  2:17560/1‑140 (MQ=255)                                                    aCCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACgggg                                                                           >  2:1102277/1‑140 (MQ=255)                                                     ccACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGa                                                                          <  2:1016541/140‑1 (MQ=255)                                                       acaGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATa                                                                        <  2:530912/140‑1 (MQ=255)                                                       acaGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATa                                                                        <  1:226363/140‑1 (MQ=255)                                                         aGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATAcc                                                                      >  1:844347/1‑140 (MQ=255)                                                           cTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCTTTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCAc                                                                    <  2:1060036/140‑1 (MQ=255)                                                                   tGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCt                                                            >  1:425893/1‑140 (MQ=255)                                                                     ttCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCtgg                                                          >  2:110212/1‑140 (MQ=255)                                                                       cGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCtggtg                                                        <  2:100480/140‑1 (MQ=255)                                                                             tAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGCACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGAtc                                                  <  2:844347/140‑1 (MQ=255)                                                                             tAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGAtc                                                  <  2:939738/140‑1 (MQ=255)                                                                             tAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGAtc                                                  >  2:69317/1‑140 (MQ=255)                                                                               aGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGAtctc                                                >  1:822721/1‑140 (MQ=255)                                                                               aGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGAtctc                                                >  1:730310/1‑140 (MQ=255)                                                                               aGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGAtctc                                                >  1:1079602/1‑140 (MQ=255)                                                                                    tGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCAc                                           >  2:963064/1‑140 (MQ=255)                                                                                    tGCAAGCACGGTTTTCAGTGCCGCTCCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCAc                                           >  1:49555/1‑140 (MQ=255)                                                                                      cAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCg                                         >  1:266294/1‑140 (MQ=255)                                                                                             ggTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCTCTCTACGCTGGTGGCGATCTCTTCACCGGTAgcgc                                  <  2:49555/140‑1 (MQ=255)                                                                                                   cAGTGCCGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCa                            <  2:243686/140‑1 (MQ=255)                                                                                                        ccGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGcc                       >  1:400645/1‑140 (MQ=255)                                                                                                         cGCTTCCATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCa                      >  2:660702/1‑140 (MQ=255)                                                                                                              ccATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCt                 <  2:1079602/140‑1 (MQ=255)                                                                                                              ccATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCt                 <  2:822721/140‑1 (MQ=255)                                                                                                              ccATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCt                 <  2:808474/140‑1 (MQ=255)                                                                                                              ccATTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCAAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCt                 <  2:1069295/140‑1 (MQ=255)                                                                                                                aTTGACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCg               <  2:730310/140‑1 (MQ=255)                                                                                                                   gACTCAATGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAgtggactg            <  1:441430/140‑8 (MQ=255)                                                                                                                      tCAACGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCGCACAGa         >  1:1061706/1‑140 (MQ=255)                                                                                                                        aaTGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCGCACAGAAt       >  2:545062/1‑140 (MQ=255)                                                                                                                        aaTGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCGCACAGAAt       >  2:849881/1‑140 (MQ=255)                                                                                                                        aaTGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCGCACAGAAt       >  1:863245/1‑140 (MQ=255)                                                                                                                        aaTGTAGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCGCACAGAAt       >  2:282075/1‑140 (MQ=255)                                                                                                                             aGTCCCGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTg  >  2:55094/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                        TCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTG  >  NC_000913/69756‑70019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑