Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,628,174	T→C	S220P (TCA→CCA)	ydfG →	3‑hydroxy acid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,628,174	0	T	C	100.0%	153.0 / NA	43	S220P (TCA→CCA)	ydfG	3‑hydroxy acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/26);  total (17/26)

GGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAA  >  NC_000913/1628048‑1628307                                                                                                                               |                                                                                                                                      ggTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGctgcctgc                                                                                                                          >  1:2208/1‑140 (MQ=255)  gTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCt                                                                                                                         <  1:91520/140‑1 (MQ=255)  gTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCt                                                                                                                         <  1:788747/140‑1 (MQ=255)   tCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTc                                                                                                                        <  2:268744/140‑1 (MQ=255)   tCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTc                                                                                                                        <  2:625258/140‑1 (MQ=255)   tCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTc                                                                                                                        >  1:459399/1‑140 (MQ=255)      ggtgggtgGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACg                                                                                                                     <  2:371856/140‑1 (MQ=255)       gtgggtggTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGt                                                                                                                    >  1:381450/1‑140 (MQ=255)        tgggtggTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGtc                                                                                                                   <  2:459399/140‑1 (MQ=255)                 ccGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGtcaatatcaat                                                                                                          >  2:411218/1‑140 (MQ=255)                 ccGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGtcaatatcaat                                                                                                          >  1:106705/1‑140 (MQ=255)                 ccGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGtcaatatcaat                                                                                                          >  2:837583/1‑140 (MQ=255)                  cGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGtcaatatcaata                                                                                                         <  2:2208/140‑1 (MQ=255)                         tCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGa                                                                                                  >  1:484755/1‑140 (MQ=255)                         tCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGa                                                                                                  >  2:18653/1‑140 (MQ=255)                         tCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGa                                                                                                  >  2:999652/1‑140 (MQ=255)                          ccAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGaa                                                                                                 <  2:627246/140‑1 (MQ=255)                                tCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAatgatg                                                                                           >  2:826343/1‑140 (MQ=255)                                     tttAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGt                                                                                      <  2:106705/140‑1 (MQ=255)                                          aGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTAccc                                                                                 <  2:587832/140‑1 (MQ=255)                                              gATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAg                                                                             <  2:409909/140‑1 (MQ=255)                                              gATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAg                                                                             <  2:381450/140‑1 (MQ=255)                                              gATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAg                                                                             >  1:806088/1‑140 (MQ=255)                                                tGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCt                                                                           <  2:484755/140‑1 (MQ=255)                                                    ggTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGc                                                                       <  1:999652/140‑1 (MQ=255)                                                    ggTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGc                                                                       <  2:806088/140‑1 (MQ=255)                                                      tAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCg                                                                     <  1:53527/140‑1 (MQ=255)                                                        aaGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGa                                                                   <  1:1069012/140‑1 (MQ=255)                                                              aaaaaaCCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGc                                                                       >  2:311869/1‑130 (MQ=255)                                                              aaaaaaCCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGc                                                                       <  1:311869/130‑1 (MQ=255)                                                                         cAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCa                                                  <  1:930699/140‑1 (MQ=255)                                                                            aaTACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTa                                               <  1:149907/140‑1 (MQ=255)                                                                                      cATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATAc                                     >  1:954161/1‑140 (MQ=255)                                                                                        ttGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATAccc                                   >  2:285495/1‑140 (MQ=255)                                                                                            cGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCg                               <  1:301725/140‑1 (MQ=255)                                                                                                  aagaTGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCTGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTg                         >  2:968554/1‑140 (MQ=255)                                                                                                   agaTGTCAGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGc                        <  1:411218/140‑1 (MQ=255)                                                                                                          aGCGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTa                 >  1:250497/1‑140 (MQ=255)                                                                                                            cGAAGCCGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTAtt               >  2:153319/1‑140 (MQ=255)                                                                                                                  cGTCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGt         <  2:1143742/140‑1 (MQ=255)                                                                                                                    tCTGGTGGGTGCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTca       <  1:153319/140‑1 (MQ=255)                                                                                                                         tgggtgCCAACGCTGCCTGCTCACGTCAATATCAATGCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACaaaa  <  1:549228/140‑1 (MQ=255)                                                                                                                         tgggtgCCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACaaaa  <  1:837583/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                      GGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAA  >  NC_000913/1628048‑1628307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑