Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,666,880	T→C	T114A (ACT→GCT)	ynfK ←	putative dethiobiotin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,666,880	0	T	C	100.0%	205.6 / NA	59	T114A (ACT→GCT)	ynfK	putative dethiobiotin synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/25);  total (34/25)

CGCCTGAGCTGTTAGCAGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTG  >  NC_000913/1666746‑1667008                                                                                                                                       |                                                                                                                                 cGCCTGAGCTGTTAGCAGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCtt                                                                                                                             <  1:704794/140‑1 (MQ=255) cGCCTGAGCTGTTAGCAGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCtt                                                                                                                             <  2:696146/140‑1 (MQ=255)              aGCAGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATg                                                                                                                >  2:143275/1‑140 (MQ=255)                     cATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACt                                                                                                         >  1:1137902/1‑140 (MQ=255)                      aTGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACtt                                                                                                        >  1:78239/1‑140 (MQ=255)                      aTGGGTAATGCAACCTTCCTGAATACCGACAACCAACAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACtt                                                                                                        >  2:392970/1‑140 (MQ=255)                          ttAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTc                                                                                                    >  1:1161766/1‑140 (MQ=255)                             aTGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGt                                                                                                 >  1:628343/1‑140 (MQ=255)                              tGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTATCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTc                                                                                                >  2:79944/1‑140 (MQ=255)                                cAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAg                                                                                              <  2:1216931/140‑1 (MQ=255)                                  aCCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGt                                                                                            >  2:701932/1‑140 (MQ=255)                                   ccTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCgg                                                                                                  >  1:1215121/1‑133 (MQ=255)                                   ccTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCgg                                                                                                  <  2:1215121/133‑1 (MQ=255)                                    cTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGttt                                                                                          >  1:78181/1‑140 (MQ=255)                                      tCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGc                                                                                        >  1:79818/1‑140 (MQ=255)                                        cTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCa                                                                                      >  1:142127/1‑140 (MQ=255)                                                  aCAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGa                                                                            <  1:701932/140‑1 (MQ=255)                                                       caacAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGa                                                                       <  1:392970/137‑1 (MQ=255)                                                       cATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGa                                                                       <  1:986094/140‑1 (MQ=255)                                                       cATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACAGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGa                                                                       >  2:1069219/1‑140 (MQ=255)                                                        aTCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAg                                                                      <  1:79944/140‑1 (MQ=255)                                                         tCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAgg                                                                     >  1:469855/1‑140 (MQ=255)                                                         tCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAgg                                                                     <  2:628343/140‑1 (MQ=255)                                                          cAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAggg                                                                    >  2:1142504/1‑140 (MQ=255)                                                              cccGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTa                                                                <  2:973893/139‑1 (MQ=255)                                                                cGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAat                                                              >  2:15530/1‑140 (MQ=255)                                                                cGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAat                                                              >  1:494579/1‑140 (MQ=255)                                                                cGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAat                                                              >  1:303438/1‑140 (MQ=255)                                                                cGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAat                                                              >  1:206244/1‑140 (MQ=255)                                                                cGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAat                                                              >  1:896543/1‑140 (MQ=255)                                                                  gCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAattg                                                            >  1:943821/1‑140 (MQ=255)                                                                        gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTggg                                                      <  1:878600/140‑1 (MQ=255)                                                                        gttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTggg                                                      <  2:1137902/140‑1 (MQ=255)                                                                         ttCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGc                                                     <  2:659585/140‑1 (MQ=255)                                                                                 cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTgc                                             <  1:42493/140‑1 (MQ=255)                                                                                 cTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCACCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTgc                                             >  1:468335/1‑140 (MQ=255)                                                                                   aCCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTgcgc                                           >  1:97126/1‑140 (MQ=255)                                                                                    cccATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGcc                                          <  1:1142504/140‑1 (MQ=255)                                                                                    cccATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGcc                                          >  1:872612/1‑140 (MQ=255)                                                                                        ttCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGc                                      >  2:367719/1‑140 (MQ=255)                                                                                         tCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCt                                     >  2:67937/1‑140 (MQ=255)                                                                                              gagaGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACttt                                >  1:559323/1‑140 (MQ=255)                                                                                              gagTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACttt                                >  1:6840/1‑140 (MQ=255)                                                                                              gagTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGCTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACttt                                >  2:776318/1‑140 (MQ=255)                                                                                                gTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTttct                              >  1:1036229/1‑140 (MQ=255)                                                                                                  ggACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTttcttc                            <  2:872612/140‑1 (MQ=255)                                                                                                  ggACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTttcttc                            <  1:287340/140‑1 (MQ=255)                                                                                                   gACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTttcttct                           >  1:742921/1‑140 (MQ=255)                                                                                                   gACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTttcttct                           >  2:355818/1‑140 (MQ=255)                                                                                                   gACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTttcttct                           >  2:1014091/1‑140 (MQ=255)                                                                                                         aaTCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCtt                     <  1:1069219/140‑1 (MQ=255)                                                                                                          aTCATTCATCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTa                    >  2:293861/1‑140 (MQ=255)                                                                                                           tcattcatCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTaa                   <  2:1161766/140‑1 (MQ=255)                                                                                                              ttcatCAGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAAcgc                >  1:931678/1‑140 (MQ=255)                                                                                                               tcatcaGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAAcgcg               <  2:469855/140‑1 (MQ=255)                                                                                                                catcaGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGCTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAACGCGa              <  1:776318/140‑1 (MQ=255)                                                                                                                  tcaGACTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAACGCGATa            <  2:648838/140‑1 (MQ=255)                                                                                                                       cTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAACGCGATAGGAtt       <  2:742921/140‑1 (MQ=255)                                                                                                                       cTGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAACGCGATAGGAtt       <  2:142127/140‑1 (MQ=255)                                                                                                                        tGCGCCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTa      <  1:15530/140‑1 (MQ=255)                                                                                                                         gcgcCAGCCGCCAGCCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTaa     <  2:79818/140‑1 (MQ=255)                                                                                                                           gccagccgccagccCCTTCTACCACGACATGATCGAC‑TTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTg  <  2:78239/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 CGCCTGAGCTGTTAGCAGTGCATGGTTAATGCAACCTTCCTGAATACCGACAACCATCAACACCGGCAGTTGTTCCTGCACTACCCATTCAGAGAGTGGACGCAAATCATTCATCAGACTGCGCCAGCCGCCAGTCCCTTCTACCACGACATGATCGACTTTTTCGGTCAGGTTTGCCAGGCCGTTTGAAATGAGGGTGTAATTGATTGGGCAACTGTGCGCCACGCTACTTTCTTCTTCGCTTAACGCGATAGGATTAACTG  >  NC_000913/1666746‑1667008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑