Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,692,622	A→G	V77A (GTT→GCT)	uidC ←	outer membrane porin family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,692,622	0	A	G	100.0%	200.8 / NA	56	V77A (GTT→GCT)	uidC	outer membrane porin family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/29);  total (27/29)

AGCAGGCTATTTTCACTGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCG  >  NC_000913/1692487‑1692760                                                                                                                                        |                                                                                                                                           aGCAGGCTATTTTCACTGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGcc                                                                                                                                        <  2:626302/140‑1 (MQ=255)  gCAGGCTATTTTCACTGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCa                                                                                                                                       >  1:429507/1‑140 (MQ=255)    aGGCTATTTTCACTGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCaat                                                                                                                                     <  2:1018475/140‑1 (MQ=255)      gCTATTTTCACTGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCaa                                                                                                                                      <  1:1011637/137‑1 (MQ=255)      gCTATTTTCACTGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCaa                                                                                                                                      >  2:1011637/1‑137 (MQ=255)       cTATTTTCACTGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCaataat                                                                                                                                  <  2:635620/140‑1 (MQ=255)           tttCACTGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTg                                                                                                                              <  2:233653/140‑1 (MQ=255)             tCACTGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGa                                                                                                                            >  2:753451/1‑140 (MQ=255)                cTGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGaata                                                                                                                         <  2:429507/140‑1 (MQ=255)                cTGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGaata                                                                                                                         <  2:401595/140‑1 (MQ=255)                 tGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGaataa                                                                                                                        >  1:649242/1‑140 (MQ=255)                    gTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAAtaataa                                                                                                                     <  1:1176413/140‑1 (MQ=255)                     ttttATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAAc                                                                                                                    <  2:799645/140‑1 (MQ=255)                             ttACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTg                                                                                                            >  2:204059/1‑140 (MQ=255)                             ttACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTg                                                                                                            <  1:300413/140‑1 (MQ=255)                                  gCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATc                                                                                                       <  2:726190/140‑1 (MQ=255)                                    ccccAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGa                                                                                                     >  1:1071507/1‑140 (MQ=255)                                          ggCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAAtc                                                                                               <  1:894763/140‑1 (MQ=255)                                          ggCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAAtc                                                                                               <  2:1017787/140‑1 (MQ=255)                                          ggCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAAtc                                                                                               <  2:946495/140‑1 (MQ=255)                                            cAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAAtcct                                                                                             <  2:45604/140‑1 (MQ=255)                                            cAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAAtcct                                                                                             <  2:419403/140‑1 (MQ=255)                                            cAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAAtcct                                                                                             <  2:280312/140‑1 (MQ=255)                                              aaGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAAtcctcc                                                                                           >  2:1180530/1‑140 (MQ=255)                                               aGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAg                                                                                                    <  2:517276/130‑1 (MQ=255)                                               aGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTccc                                                                                          >  2:314955/1‑140 (MQ=255)                                               aGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTccc                                                                                          >  1:1167552/1‑140 (MQ=255)                                               aGCCAAAACTTTTATCACCATCAAGAAACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAg                                                                                                    >  1:517276/1‑130 (MQ=255)                                                     aaCTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTAcc                                                                                    <  1:717310/140‑1 (MQ=255)                                                      aCTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTAccc                                                                                   <  2:649242/140‑1 (MQ=255)                                                          ttATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGc                                                                               <  1:834926/140‑1 (MQ=255)                                                             tCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTa                                                                            <  1:500043/140‑1 (MQ=255)                                                                  aTCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAatat                                                                       >  2:1208365/1‑140 (MQ=255)                                                                            caccGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCcgc                                                             >  2:381089/1‑140 (MQ=255)                                                                             accGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTG‑AATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCgcgc                                                           <  2:636800/140‑1 (MQ=255)                                                                              ccGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCgcgc                                                           <  1:510573/140‑1 (MQ=255)                                                                                 ggTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGGGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAg                                                        >  1:98465/1‑140 (MQ=255)                                                                                 ggTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAg                                                        >  1:356553/1‑140 (MQ=255)                                                                                 ggTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAg                                                        >  2:733524/1‑140 (MQ=255)                                                                                 ggTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAg                                                        >  1:1081356/1‑140 (MQ=255)                                                                                   tACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCa                                                      <  1:733524/140‑1 (MQ=255)                                                                                     cTCCTATCAGCACGAGCACCTAATTTATAGACCTAATACGCCCCGCCTTCACCGCCATTACTATTGGTATCATAACCACTTTTGACTTCGAGCAATCCTCCCTGTCCCCATCCGTCACTATCTCTTCCCGCGCCAGCACt                                                    >  2:264532/1‑140 (MQ=255)                                                                                           tCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCtt                                              >  2:959900/1‑140 (MQ=255)                                                                                             aGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTATCCACGCGTAAATAGCTCTAGCCGAGGCAGCACTGGGcagag                                            <  2:1071507/140‑5 (MQ=255)                                                                                             aGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTAt                                            >  2:925467/1‑140 (MQ=255)                                                                                               cACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATcc                                          >  2:567656/1‑140 (MQ=255)                                                                                               cACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATcc                                          >  1:946724/1‑140 (MQ=255)                                                                                                       ccTAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTgcg                                  >  2:695364/1‑140 (MQ=255)                                                                                                         tAATTTATAAACATAATACGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCa                                >  1:1008249/1‑140 (MQ=255)                                                                                                                          aCGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTg               <  1:567656/140‑1 (MQ=255)                                                                                                                          aCGCCCCGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTg               >  2:889532/1‑140 (MQ=255)                                                                                                                              cccGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCTCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGc           >  2:1060687/1‑140 (MQ=255)                                                                                                                              cccGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTg               <  2:1126239/136‑1 (MQ=255)                                                                                                                              cccGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTg               >  1:1126239/1‑136 (MQ=255)                                                                                                                              cccGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGc           >  1:298357/1‑140 (MQ=255)                                                                                                                              cccGCCTTCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGc           >  2:850045/1‑140 (MQ=255)                                                                                                                                    ttCAGCGCCACTAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTc     <  2:749299/140‑1 (MQ=255)                                                                                                                                     tCAGCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTcc    <  1:474918/140‑1 (MQ=255)                                                                                                                                       aaCGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCg  >  1:855562/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                           AGCAGGCTATTTTCACTGGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGCCAATAATATTGGAATAATAACCACTATTGAAATCGAGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCG  >  NC_000913/1692487‑1692760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑