Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,734,952	T→C	V173A (GTT→GCT)	mepH →	peptidoglycan DD‑endopeptidase MepH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,734,952	0	T	C	100.0%	145.6 / NA	41	V173A (GTT→GCT)	mepH	peptidoglycan DD‑endopeptidase MepH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/19);  total (22/19)

CTTGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGTTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCTTTTTCCGTACTCAG  >  NC_000913/1734819‑1735085                                                                                                                                      |                                                                                                                                      ctTGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCttatta                                                                                                                                 <  2:51573/140‑1 (MQ=255) ctTGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCttatta                                                                                                                                 <  2:670430/140‑1 (MQ=255)  ttGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAG‑CATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACg                                                                                                                               >  2:78621/1‑140 (MQ=255)           cgcACAAAGCGAAGGTACAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATaaa                                                                                                                       >  2:700354/1‑140 (MQ=255)                   gCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGt                                                                                                               <  2:1148421/140‑1 (MQ=255)                    cGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTg                                                                                                              >  1:1092054/1‑140 (MQ=255)                           gCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTc                                                                                                       <  2:1092054/140‑1 (MQ=255)                            cAAAAAGCTACGAAAGTGGCAATTAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACTCTTATAAAGATTTGGTGAAAATTCg                                                                                                      <  2:916249/140‑1 (MQ=255)                                        aaaGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTAc                                                                                          <  1:708389/140‑1 (MQ=255)                                          aGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACgg                                                                                        <  2:446994/140‑1 (MQ=255)                                              gCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGaa                                                                                    >  2:787399/1‑140 (MQ=255)                                                 aTGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGa                                                                                 <  1:84427/140‑1 (MQ=255)                                                 aTGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGa                                                                                 <  2:1111461/140‑1 (MQ=255)                                                          cTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACg                                                                                         >  1:355474/1‑123 (MQ=255)                                                          cTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACg                                                                                         <  2:355474/123‑1 (MQ=255)                                                          cTGATGCAGCAAATTGGTAAG‑CATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCAc                                                                       <  1:78621/140‑1 (MQ=255)                                                             aTGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGa                                                                                 >  1:553334/1‑128 (MQ=255)                                                             aTGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGa                                                                                 <  2:553334/128‑1 (MQ=255)                                                              tGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTg                                                                    >  2:627591/1‑140 (MQ=255)                                                                  gcaAATTGGTAAGCCGTATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTg                                                                >  2:644334/1‑140 (MQ=255)                                                                           tAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAgcgc                                                       <  1:627591/140‑1 (MQ=255)                                                                             aGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCa                                                     <  1:96425/140‑1 (MQ=255)                                                                              gCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCaa                                                    <  1:1063067/140‑1 (MQ=255)                                                                                 atatCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCg                                                 >  1:762647/1‑140 (MQ=255)                                                                                  tatCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGa                                                >  1:1140111/1‑140 (MQ=255)                                                                                   atCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGaa                                               >  1:1194855/1‑140 (MQ=255)                                                                                   atCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGAGGCCTGGCTTATTACGCTGATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGaa                                               >  2:1083981/1‑140 (MQ=255)                                                                                    tCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAAc                                              >  1:489151/1‑140 (MQ=255)                                                                                       ttGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTa                                           <  1:96941/140‑1 (MQ=255)                                                                                       ttGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTa                                           >  1:935093/1‑140 (MQ=255)                                                                                         ggggTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGt                                         >  1:248978/1‑140 (MQ=255)                                                                                               gCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTg                                   >  2:437660/1‑140 (MQ=255)                                                                                                      aCCGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACg                            <  1:1100522/140‑1 (MQ=255)                                                                                                       ccGCGTACCGGTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACgg                           <  2:248978/140‑1 (MQ=255)                                                                                                                ggTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGt                  >  1:129166/1‑140 (MQ=255)                                                                                                                 gTTTTGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTc                 >  2:615774/1‑140 (MQ=255)                                                                                                                  ttttGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCt                >  2:711082/1‑140 (MQ=255)                                                                                                                   tttGATTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCtt               <  2:489151/140‑1 (MQ=255)                                                                                                                       aTTGCAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCTTTTTc           >  1:985493/1‑140 (MQ=255)                                                                                                                           cAGCGGCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCTTTTTCCGTa       >  2:528848/1‑140 (MQ=255)                                                                                                                                gCCTGGCTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCTTTTTCCGTACTCAg  >  1:1064629/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      CTTGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGCTCACCGCGTACCGGTTTTGATTGCAGCGGCCTGGTTTATTACGCTTATAAAGATTTGGTGAAAATTCGTATTCCGCGTACGGCGAATGAAATGTATCACCTGCGTGATGCAGCGCCAATCGAACGTAGTGAACTGAAAAACGGCGACCTGGTCTTTTTCCGTACTCAG  >  NC_000913/1734819‑1735085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑