Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	99,166	A→G	E255G (GAA→GGA)	ftsW →	putative lipid II flippase FtsW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	99,166	0	A	G	100.0%	163.1 / NA	46	E255G (GAA→GGA)	ftsW	putative lipid II flippase FtsW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/25);  total (21/25)

GGCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACT  >  NC_000913/99029‑99290                                                                                                                                          |                                                                                                                             ggCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAg                                                                                                                            <  2:945097/140‑1 (MQ=255)       gttgttCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCt                                                                                                                      <  2:83036/140‑1 (MQ=255)        ttgttCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCtt                                                                                                                     >  2:759675/1‑140 (MQ=255)          gttCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTg                                                                                                                   >  1:681387/1‑140 (MQ=255)           ttCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTgg                                                                                                                  <  2:681387/140‑1 (MQ=255)            tCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGc                                                                                                                 >  1:260693/1‑140 (MQ=255)            tCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGc                                                                                                                 >  1:365628/1‑140 (MQ=255)             ccTGGCGGGAGCGAAATTTTGGCAGTTCATTGCCTTTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCa                                                                                                                <  2:27532/140‑1 (MQ=255)              cTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAg                                                                                                               <  1:396359/140‑1 (MQ=255)                  cGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGc                                                                                                           >  2:264430/1‑140 (MQ=255)                    ggAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTa                                                                                                         <  1:783165/140‑1 (MQ=255)                    ggAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTa                                                                                                         <  2:169659/140‑1 (MQ=255)                      aGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATc                                                                                                       >  1:102406/1‑140 (MQ=255)                                    aGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCg                                                                                         <  2:1080257/140‑1 (MQ=255)                                       tCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTg                                                                                      >  1:50532/1‑140 (MQ=255)                                       tCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTg                                                                                      >  2:1078599/1‑140 (MQ=255)                                        cATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGa                                                                                     <  2:256438/140‑1 (MQ=255)                                        cATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGa                                                                                     <  1:1080355/140‑1 (MQ=255)                                        cATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGa                                                                                     <  1:823891/140‑1 (MQ=255)                                        cATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGa                                                                                     <  2:789536/140‑1 (MQ=255)                                                   tCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGt                                                                          <  2:185854/140‑1 (MQ=255)                                                     ggTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTcg                                                                        >  2:295642/1‑140 (MQ=255)                                                       tATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTcgcg                                                                      >  2:519889/1‑140 (MQ=255)                                                       tATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTcgcg                                                                      >  2:1091671/1‑140 (MQ=255)                                                                ttCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACttt                                                             <  1:295642/140‑1 (MQ=255)                                                                     cGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTggggg                                                        <  2:1163451/140‑1 (MQ=255)                                                                      ggTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGc                                                       <  1:1099134/140‑1 (MQ=255)                                                                      ggTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGc                                                       <  2:272161/140‑1 (MQ=255)                                                                      ggTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGc                                                       <  1:62029/140‑1 (MQ=255)                                                                      ggTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGc                                                       <  2:365628/140‑1 (MQ=255)                                                                      ggTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGc                                                       <  1:264430/140‑1 (MQ=255)                                                                          gtgtTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAgg                                                   >  2:916875/1‑140 (MQ=255)                                                                          gtgtTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAgg                                                   >  1:548130/1‑140 (MQ=255)                                                                           tgtTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGc                                                       <  1:967656/135‑1 (MQ=255)                                                                           tgtTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGc                                                       >  2:967656/1‑135 (MQ=255)                                                                                     aCTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACt                                        >  1:785835/1‑140 (MQ=255)                                                                                         gCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGt                                                  <  1:1197608/126‑1 (MQ=255)                                                                                         gCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGt                                                  >  2:1197608/1‑126 (MQ=255)                                                                                          ccGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTa                                   >  2:722024/1‑140 (MQ=255)                                                                                           cGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTAc                                  >  1:221231/1‑140 (MQ=255)                                                                                                         tccaccgTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTg                          >  1:953618/5‑134 (MQ=255)                                                                                                         tCCACCGTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTg                          <  2:953618/134‑1 (MQ=255)                                                                                                              cgTGTTACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGcc               >  2:627992/1‑140 (MQ=255)                                                                                                                   tACCGCATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAg          <  2:902020/140‑1 (MQ=255)                                                                                                                        cATTCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGcac     >  2:744533/1‑140 (MQ=255)                                                                                                                           tCTGGAACCCGTGGGGAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACt  <  1:519889/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                             GGCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACT  >  NC_000913/99029‑99290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑